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PE/Cyanine7 anti-human CD25 Antibody

Pricing & Availability
Clone
BC96 (See other available formats)
Regulatory Status
RUO
Workshop
V T-072
Other Names
Low affinity IL-2R, IL-2R α chain, Tac, p55
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
BC96_PECy7_071805
PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with CD25 (clone BC96) PE/Cyanine7 (solid line) or mouse IgG1, κ PE/Cyanine7 isotype control (dotted line).
  • BC96_PECy7_071805
    PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with CD25 (clone BC96) PE/Cyanine7 (solid line) or mouse IgG1, κ PE/Cyanine7 isotype control (dotted line).
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
302611 25 tests 115€
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302612 100 tests 234€
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Description

CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications include: immunocytochemistry3.

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  3. Ernst CW, et al. 2007. Clin. Exp. Immunol. 148:271. (ICC) PubMed
Product Citations
  1. Sayin I, et al. 2018. J Exp Med. 7:40286. PubMed
  2. Kagoya Y, et al. 2018. Nat Commun. 9:1915. PubMed
  3. Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed
  4. Barry KC, et al. 2018. Nat Med. 24:1178. PubMed
  5. Overacre-Delgoffe AE, et al. 2017. Cell. 169:1130. PubMed
  6. Gorczynski RM, et al. 2017. Immunology. 150:418. PubMed
  7. Levring TB, et al. 2019. Sci Rep. 9:16725. PubMed
  8. Rendeiro AF, et al. 2020. Nat Commun. 11:577. PubMed
  9. Huang B, et al. 2020. Cell. 179(5):1160-1176.e24.. PubMed
  10. Evans RDR, et al. 2020. Nat Commun. 3.491666667. PubMed
  11. Wu W, et al. 2020. Oncol Lett. 0.91875. PubMed
  12. Weinberg A, et al. 2015. PLoS One. 10:122431. PubMed
  13. Groen B, et al. 2015. Sci Rep. 5: 13618. PubMed
  14. Couturier J, et al. 2016. Retroviology. 13:30. PubMed
  15. Palamides P, et al. 2016. Dis Model Mech. 9: 985 - 997. PubMed
  16. Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed
  17. Gonzalez-Figueroa P, et al. 2021. Cell. 184(7):1775-1789.e19. PubMed
  18. Obradovic A, et al. 2021. Cell. 184(11):2988-3005.e16. PubMed
  19. Acharya A, et al. 2020. J Virol. 95:e01657. PubMed
  20. Zirngibl F, et al. 2021. J Immunother Cancer. 9:. PubMed
  21. NULL, et al. 2022. Cell. 185:916. PubMed
  22. Xu X, et al. 2020. Arthritis Rheumatol. 72:1303. PubMed
  23. Massafra V, et al. 2021. J Immunol. 207:493. PubMed
  24. Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
  25. Kim ST, et al. 2022. Nat Commun. 13:1970. PubMed
  26. Yuan H, et al. 2022. J Clin Exp Hematop. 62:52. PubMed
  27. Krammer S, et al. 2022. Cells. 12: . PubMed
  28. Vondra S, et al. 2023. Cell Rep. 42:111977. PubMed
  29. Veatch JR, et al. 2022. Cancer Cell. 40:393. PubMed
  30. Gkika E, et al. 2023. NPJ Precis Oncol. 7:24. PubMed
  31. Battistello E, et al. 2023. Mol Cell. 83:1216. PubMed
  32. Patskovsky Y, et al. 2023. Nat Commun. 14:3763. PubMed
RRID
AB_314281 (BioLegend Cat. No. 302611)
AB_314282 (BioLegend Cat. No. 302612)

Antigen Details

Structure
Type I transmembrane glycoprotein, 55 kD
Distribution

Activated T cells and B cells, monocytes/macrophages, Treg

Function
Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
Ligand/Receptor
IL-2
Cell Type
B cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann T. 1991. J. Biol. Chem. 266:2681.

Gene ID
3559 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 12.15.2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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