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PE/Cyanine7 anti-human HLA-DR Antibody

Pricing & Availability
Clone
L243 (See other available formats)
Regulatory Status
RUO
Other Names
Major Histocompatibility Class II, MHC class II
Isotype
Mouse IgG2a, κ
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Product Citations
publications
L243_PECy7_072607
Human peripheral blood lymphocytes stained with L243 PE/Cyanine7
  • L243_PECy7_072607
    Human peripheral blood lymphocytes stained with L243 PE/Cyanine7
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
307615 25 tests 123€
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307616 100 tests 249€
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Description

HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kD α (heavy) chain and a 27 kD β (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells, and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The L243 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Clone L243 binds a conformational epitope on HLA-DRa which depends on the correct folding of the aß heterodimer.19

Additional reported applications (for the relevant formats) include: immunoprecipitation8, Western blotting8, in vitro blocking of mixed lymphocyte reactions9,10, depeletion of MHC class II cells7, immunohistochemical staining of acetone-fixed frozen sections4,5, and spatial biology (IBEX)21,22. For sensitive functional assays, we recommend using the Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 307648, 307665 - 307669).

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References
  1. Brodsky F. 1984. Immunogenetics 19:179.
  2. Robbins P, et al. 1987. Human Immunol. 18:301.
  3. Stites D, et al. 1986. Clin. Immunol. Immunopathol. 38:161.
  4. Warnke R, et al. 1980. J. Histochem. Cytochem. 28:771. (IHC)
  5. Engleman E, et al. 1981. P. Natl. Acad. Sci. USA 78:1791. (IHC)
  6. Zipf T, et al. 1981. Cancer Res. 41:4786.
  7. Goodier M, et al. 2000. J. Immunol. 165:139. (Depletion)
  8. Esser M, et al. 2001. J. Virol. 75:6173. (IP, WB)
  9. Kalka-Moll WM, et al. 2002. J. Immunol. 169:6149. (Block)
  10. Wang RF, et al. 1999. Science 284:1351. (Block)
  11. Zaba LC, et al. 2007. J. Exp. Med. 204:3183. PubMed
  12. Fujita H, et al. 2009. P. Natl. Acad. Sci. USA 106:21795. PubMed
  13. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  14. Goncalves RM, et al. 2010. Infect. Immun. 78:4763. PubMed
  15. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  16. Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
  17. Stein R, et al. 2011. Leuk. Lymphoma 52:273.
  18. Galkowska H, et al. 1996. Vet. Immunol. Immunopathol. 53:329.
  19. Moro M, et al. 2005. BMC Immunol. 6:24.
  20. Lauterbach N, et al. 2014. Mol Immunol. 59:19. PubMed
  21. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  22. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Du X, et al. 2018. Cell Res. 28:416. PubMed
  2. Neff CP et al. 2018. EBioMedicine. 30:192-202 . PubMed
  3. Orengo JM, et al. 2018. Nat Commun. 9:1421. PubMed
  4. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  5. Ivan Jelcic et al. 2018. Cell. 175(1):85-100 . PubMed
  6. Alissafi T, et al. 2018. J Clin Invest. 128:3840. PubMed
  7. Luo N, et al. 2018. Nat Commun. 0.547222222. PubMed
  8. Mastoridis S, et al. 2018. Front Immunol. 1.474305556. PubMed
  9. Zhang M, et al. 2018. Virol J. 0.735416667. PubMed
  10. Martín–Gayo E, et al. 2020. Cell Rep. 30:984. PubMed
  11. Domingos-Pereira S, et al. 2019. Cancer Immunol Res. 7:621. PubMed
  12. Mothe B, et al. 2020. Front Immunol. 1.029861111. PubMed
  13. Causi E, et al. 2015. PLoS One. 10: 0136717. PubMed
  14. Johnson D, et al. 2016. Nat Commun. 7:10582. PubMed
  15. Macal M, et al. 2016. J Immunol. 196: 1900 - 1909. PubMed
  16. Pennington L, et al. 2016. Nat Commun. 7:11610. PubMed
  17. Barman S, et al. 2016. Int Immunol. 28: 533 - 545. PubMed
  18. Gibellini L, et al. 2020. EMBO Mol Med. 12:e13001. PubMed
  19. Garrido-Martin EM, et al. 2020. J Immunother Cancer. 8:00. PubMed
  20. Woo MS, et al. 2021. iScience. 24(7):102752. PubMed
  21. Lindenbergh MFS, et al. 2020. J Extracell Vesicles. 1798606:9. PubMed
  22. Platten M, et al. 2021. Nature. 592:463. PubMed
  23. Carre C, et al. 2021. iScience. 24:102970. PubMed
  24. Zhao X, et al. 2022. Front Mol Biosci. 9:786864. PubMed
  25. Zheng H, et al. 2022. Cell Rep. 39:110864. PubMed
  26. Port JR, et al. 2020. J Virol. 94:. PubMed
  27. Muto S, et al. 2021. Oncol Lett. 21:203. PubMed
  28. Singh VK, et al. 2022. Front Immunol. 13:865503. PubMed
  29. Panigrahi S, et al. 2020. Eur J Immunol. 50:2055. PubMed
  30. Campbell VL, et al. 2020. Immunohorizons. 4:444. PubMed
  31. Wei H, et al. 2022. Front Immunol. 13:1060695. PubMed
  32. Arenas-Luna VM, et al. 2022. Curr Issues Mol Biol. 45:268. PubMed
  33. Duhalde Vega M, et al. 2022. Sci Adv. 8:eabn6545. PubMed
  34. Freund EC, et al. 2023. Methods Mol Biol. 2618:201. PubMed
  35. Shen WY, et al. 2023. iScience. 26:106805. PubMed
RRID
AB_493589 (BioLegend Cat. No. 307615)
AB_493588 (BioLegend Cat. No. 307616)

Antigen Details

Structure
Ig superfamily, MHC class II, heterodimeric transmembrane protein, 36 kD heavy and 27 kD light chain
Distribution

B cells, activated T cells, monocytes/macrophages, dendritic cells, other APCs

Function
Peptide presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Levacher M, et al. 1990. Clin. Exp. Immunol. 81:177.
2. Terstappen L, et al. 1990. J. Leukocyte Biol. 48:138.
3. Edwards JA, et al. 1986. J. Immunol. 137:490.
4. van Es A, et al. 1984. Transplantation 37:65.
5. O'Doherty U, et al. 1994. Immunology 82:487.
6. Thomas R, et al. 1994. J. Immunol. 153:4016.
7. Grouard G, et al. 1996. Nature 384:364.

Gene ID
3122 View all products for this Gene ID 3123 View all products for this Gene ID
UniProt
View information about HLA-DR on UniProt.org
Go To Top Version: 2    Revision Date: 06.17.2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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