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PerCP anti-human CD3 Antibody

Pricing & Availability
Clone
SK7 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
T3, CD3ε
Isotype
Mouse IgG1, κ
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Product Citations
publications
SK7_PerCP_090310
Human peripheral blood lymphocytes stained with SK7 PerCP
  • SK7_PerCP_090310
    Human peripheral blood lymphocytes stained with SK7 PerCP
See PerCP spectral data
Cat # Size Price Quantity Check Availability Save
344813 25 tests 115€
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344814 100 tests 252€
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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections4,5,8, immunofluorescent staining6, and Western blotting3.

Application References
  1. Kan EA, et al. 1983. J. Immunol. 131:536.
  2. Wood GS, et al. 1985. Am. J. Pathol. 120:371.
  3. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  4. Haringman JJ, et al. 2005. Arthritis Res. Ther. 7:R862. (IHC)
  5. Carbone A, et al. 1999. Blood 93:2319. (IHC)
  6. Goval JJ, et al. 2006. J. Histochem. Cytochem. 54:75. (IF)
  7. Rutjens E, et al. 2007. J. Immunol. 178:1702.
  8. Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC)
  9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Product Citations
  1. Fowler DW, et al. 2017. Cancer Immunol Immunother. 66:1205. PubMed
  2. Giesecke C, et al. 2017. Cytometry A. 10.1002/cyto.a.23250. PubMed
  3. Turaj AH et al. 2017. Cancer cell. 32(6):777-791 . PubMed
  4. Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
  5. Mozhgani SH, et al. 2019. Retrovirology. 16:46. PubMed
  6. Kang HM, et al. 2018. Nat Biotechnol. 36:89. PubMed
  7. Corrah T, et al. 2011. J Virol. 85:3367. PubMed
  8. Borhis G, et al. 2013. J Immunol. 190:2924. PubMed
  9. Srinivasan A, et al. 2014. Biol Blood Marrow Transplant. 20:1224. PubMed
  10. Gooneratne S, et al. 2015. J Virol. 89:97. PubMed
  11. Madhavi V, et al. 2015. J Infect Dis. 211:529. PubMed
  12. Cuesta M, et al. 2016. J Immunol. 196: 2466 - 2475. PubMed
  13. Dockree T, et al. 2016. Immunol Cell Biol. 95:68-76. PubMed
  14. Eriksson E, et al. 2016. Gene Ther. 10.1038/gt.2016.80. PubMed
  15. Cohen Z, et al. 2020. Biomed Rep. 13:17. PubMed
  16. Keck S, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. 12(2):507-545. PubMed
  17. Reif T, et al. 2021. Cell Reports Medicine. 2(6):100317. PubMed
  18. Arra A, et al. 2021. Oncoimmunology. 10:1938475. PubMed
  19. Kaewchim K, et al. 2021. Molecules. 26:. PubMed
  20. Misheva M, et al. 2022. Nat Commun. 13:139. PubMed
  21. Tarke A, et al. 2022. Cell. 185:847. PubMed
  22. Wu K, et al. 2021. Aging (Albany NY). 13:. PubMed
  23. Weskamm LM, et al. 2022. Cell Rep Med. 3:100685. PubMed
  24. Weskamm LM, et al. 2022. STAR Protoc. 3:101902. PubMed
  25. Sun P, et al. 2022. iScience. 25:105202. PubMed
RRID
AB_10641841 (BioLegend Cat. No. 344813)
AB_10639948 (BioLegend Cat. No. 344814)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ, (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, during thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

How stable is PerCP/Cy5.5 tandem as compared to PerCP alone?

PerCP/Cy5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 11.30.2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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