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PerCP/Cyanine5.5 anti-human CD19 Antibody

Pricing & Availability
Clone
HIB19 (See other available formats)
Regulatory Status
RUO
Workshop
V CD19.11
Other Names
B4
Isotype
Mouse IgG1, κ
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Product Citations
publications
HIB19_PerCPCy55_CD19_Antibody_101618
Human peripheral blood lymphocytes were stained with CD19 (clone HIB19) PerCP/Cyanine5.5 (filled histogram), or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • HIB19_PerCPCy55_CD19_Antibody_101618
    Human peripheral blood lymphocytes were stained with CD19 (clone HIB19) PerCP/Cyanine5.5 (filled histogram), or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
302229 25 tests 109€
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302230 100 tests 231€
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Description

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections8 and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302267 & 302268).

 

Clone HIB19 partially blocks anti-human CD19 clones 4G7 and SJ25C1 staining based on in-house testing

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Bradbury L, et al. 1993. J. Immunol. 151:2915.
  4. Joseph A, et al. 2010. J. Virol. 84:6645. PubMed
  5. Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed
  6. Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Ivan Jelcic et al. 2018. Cell. 175(1):85-100 . PubMed
  2. Walk J, et al. 2019. Nat Commun. 10:874. PubMed
  3. Calzoni E, et al. 2019. J Allergy Clin Immunol. 143:2317. PubMed
  4. Adhikaree J, et al. 2019. Oncoimmunology. 8:1593803. PubMed
  5. Gawel DR, et al. 2019. Genome Med. 11:47. PubMed
  6. Munteanu AN, et al. 2019. Exp Ther Med. 18:1693. PubMed
  7. Zumaquero E, et al. 2019. Elife. 8. PubMed
  8. Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
  9. Körner C et al. 2017. Cell host & microbe. 22(1):111-119 . PubMed
  10. Sullivan KD et al. 2016. eLife. 5 pii: e16220. PubMed
  11. Rölle A, et al. 2018. Cell Rep. 24:1967. PubMed
  12. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  13. Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed
  14. Cella M, et al. 2019. Nat Immunol. 1.513888889. PubMed
  15. Huang N, et al. 2020. Genome Biol. 1.03125. PubMed
  16. Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
  17. Lepore M, et al. 2014. J Exp Med. 211:1363. PubMed
  18. Garcia-Carmona Y, et al. 2015. Blood. 125:1749. PubMed
  19. Inui M, et al. 2015. Int Immunol. 27: 345 - 355. PubMed
  20. Teirlinck A, et al. 2015. Infect Immun . 83: 3732-3739. PubMed
  21. Fraser H, et al. 2015. J Immunol. 195: 4841 - 4852. PubMed
  22. Lima J, et al. 2016. Reprod Sci. 10.1177/1933719116653680. PubMed
  23. Palamides P, et al. 2016. Dis Model Mech. 9: 985 - 997. PubMed
  24. Lubaki N, et al. 2016. PLoS Pathog. 12:e1006031. PubMed
  25. Ehlers L, et al. 2021. The FASEB Journal. 35(7):e21684. PubMed
  26. Japp AS, et al. 2021. Cell. 184(3):827-839.e14. PubMed
  27. Graham C, et al. 2021. Immunity. 54(6):1276-1289.e6. PubMed
  28. Motozono C, et al. 2021. Cell Host Microbe. . PubMed
  29. Li M, et al. 2020. Nat Commun. 4051:11. PubMed
  30. Duhen R, et al. 2021. Nat Commun. 12:1047. PubMed
  31. Sweeney CL, et al. 2021. Gene Ther. 28:373. PubMed
  32. Fucà G, et al. 2021. J Immunother Cancer. 9:. PubMed
  33. Lerrer S, et al. 2021. iScience. 24:103020. PubMed
  34. Afolabi LO, et al. 2021. Front Immunol. 12:701671. PubMed
  35. Rice TF, et al. 2021. EBioMedicine. 72:103612. PubMed
  36. Ramendra R, et al. 2021. Front Immunol. 12:694152. PubMed
  37. Kimura I, et al. 2022. Cell Rep. 38:110218. PubMed
  38. Sung BY, et al. 2022. J Clin Invest. 132:. PubMed
  39. Duncan CJA, et al. 2022. J Exp Med. 219:. PubMed
  40. Seow J, et al. 2022. Cell Rep. 39:110757. PubMed
  41. Lev A, et al. 2021. J Exp Med. 218:. PubMed
  42. Abolhalaj M, et al. 2022. Cancer Med. 11:3023. PubMed
  43. He WT, et al. 2022. Nat Immunol. 23:960. PubMed
  44. Herter JM, et al. 2022. Strahlenther Onkol. Online ahead of print. PubMed
  45. Yuan H, et al. 2022. J Clin Exp Hematop. 62:52. PubMed
  46. Höfle J, et al. 2022. EMBO Rep. 23:e54133. PubMed
  47. He WT, et al. 2022. Sci Transl Med. 14:eabl9605. PubMed
  48. Li M, et al. 2023. Front Immunol. 14:1087923. PubMed
  49. Abarca-Zabalía J, et al. 2023. Front Immunol. 14:1127352. PubMed
  50. Zhou P, et al. 2023. Immunity. 56:669. PubMed
RRID
AB_2275547 (BioLegend Cat. No. 302229)
AB_2073119 (BioLegend Cat. No. 302230)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution

B lineage (except plasma cells), follicular dendritic cells

Function
B cell activation and differentiation
Ligand/Receptor
Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.

Gene ID
930 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 10.16.2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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