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Purified anti-HA.11 Epitope Tag Antibody (Previously Covance catalog# PRB-101P)

Pricing & Availability
Clone
Poly9023 (See other available formats)
Regulatory Status
RUO
Other Names
HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag
Previously
Covance Catalog# PRB-101P
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Purified_HA.11_WB_021315
Western Blot showing specificity of HA.11, clone Poly9023, at 1:1000 and 1:2000. Lane 1: Molecular weight marker. Lane 2: CHO Clone 6. Lane 3: BSA negative control.
  • Purified_HA.11_WB_021315
    Western Blot showing specificity of HA.11, clone Poly9023, at 1:1000 and 1:2000. Lane 1: Molecular weight marker. Lane 2: CHO Clone 6. Lane 3: BSA negative control.
Cat # Size Price Quantity Check Availability Save
902303 25 µL 72€
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902301 200 µL 359€
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902302 500 µL 565€
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Description

The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106.  It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Polyclonal antibody HA.11 was raised against the twelve amino acid sequence CYPYDVPDYASL.
Formulation
Phosphate-buffered solution + 0.03% thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB, IP
ChIP, ICC, Purification - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition.
• WB: 1:1,000*
• IP: 1:150

Application Notes

This antibody is effective in immunoblotting (WB) and immunoprecipitation (IP) of tagged proteins.

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

This antibody recognizes the same hemagglutinin flu epitope YPYDVPDYA as the monoclonal antibody, HA.11 clone 16B12. This polyclonal antibody facilitates epitope tag experiments in murine systems and is useful in experiments involving two proteins labeled with two different tags.

This product may contain other non-IgG subtypes.

Application References
  1. Barrott JJ, et al. 2011. PNAS. 108: 12752. (WB, IP)
  2. Pecot MY, Malhotra V. 2004. Cell. 116:99.
  3. Delmas C, et al. 2003. J Biol Chem. 278:12443. (IF)
  4. Caplan S, et al. 2001. J Cell Biol. 154:109. (IF, WB)
  5. Yeo C, Whitman M. 2001. Mol Cell 7:949.
  6. Sander TL, et al. 2000. J Biol Chem.:12857. (WB)
  7. Bosshart H, et al. 1994. J Cell Biol. 126:1157. (IF, IP)
  8. Hewawasam G, et al. 2010. Mol Cell. 40:444. (ChIP, WB) PubMed
  9. Hurov JB, et al. 2004. Curr Biol. 14:736. (IF)
  10. Shieh AW, et al. 2013. PLoS One. 8:75337. (WB, IF) PubMed
  11. Hackenbeck T, et al. 2009. Cell Cycle. 8:1386. (ICC)
  12. Satheshkumar PS, et al. 2013. J Virol. 87:10710.
  13. Luo R, et al. 2009. Cell Signal. 21:1169. (WB) PubMed
  14. Berg TJ, et al. 2010. J Biol Chem. 285:35255. (WB) PubMed
Product Citations
  1. Judith D, et al. 2019. J Cell Biol. 218:1634. PubMed
  2. Wang Q, et al. 2019. Biomolecules. 9:4. PubMed
  3. Coulombe P et al. 2019. Cell reports. 27(6):1769-1780 . PubMed
  4. Choi Y, et al. 2019. Nat Microbiol. 4:429. PubMed
  5. Choi YJ, et al. 2019. Nat Immunol. 20:276. PubMed
  6. Robles‐Valero J et al. 2017. Cancer cell. 32(5):608-623 . PubMed
  7. Dafinger C, et al. 2018. Exp Mol Med. 50:75. PubMed
  8. Nissim S, et al. 2019. Nat Genet. 51:1308. PubMed
  9. Chalker A 2013. PLoS One. 8:e75337. PubMed
  10. Hewawasam G, et al. 2010. Mol Cell. 40:444-454. PubMed
  11. Luo R, et al. 2009. Cell Signal. 21:1169-1179. PubMed
  12. Berg T, et al. 2010. J Biol Chem. 285:35255-32566. PubMed
  13. Bartram M, et al. 2016. Hum Mol Genet. 25: 1152 - 1164. PubMed
  14. Rinschen M, et al. 2016. Hum Mol Genet. 25: 1328-1344. PubMed
  15. Tsao N, et al. 2016. Biochem J. 473: 1237 - 1245. PubMed
  16. Liu X, et al. 2021. Nat Commun. 12:4427. PubMed
  17. Ho TYH, et al. 2021. Nat Commun. 12:2200. PubMed
  18. Patchett S, et al. 2021. Cell Rep. 36:109754. PubMed
  19. Szilágyi SS, et al. 2022. BMC Biol. 20:50. PubMed
  20. Martin CK, et al. 2022. Viruses. 14:. PubMed
  21. Morales L, et al. 2022. Front Neural Circuits. 16:831074. PubMed
  22. Pal D, et al. 2022. Commun Biol. 5:646. PubMed
  23. Yuan G, et al. 2022. ACS Synth Biol. 11:2513. PubMed
  24. Suzuki R, et al. 2023. Microbiol Immunol. 67:22. PubMed
  25. Pal D, et al. 2023. iScience. 26:106274. PubMed
  26. Choi YJ, et al. 2023. Nat Commun. 14:2045. PubMed
  27. Xiao X, et al. 2023. Nat Commun. 14:2859. PubMed
  28. Yuan G, et al. 2023. Commun Biol. 6:567. PubMed
RRID
AB_2734670 (BioLegend Cat. No. 902303)
AB_2565018 (BioLegend Cat. No. 902301)
AB_2565019 (BioLegend Cat. No. 902302)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about HA.11 on UniProt.org
Go To Top Version: 6    Revision Date: 01.25.2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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