Loading...

Purified anti-human CD127 (IL-7Rα) Antibody

Pricing & Availability
Clone
A019D5 (See other available formats)
Regulatory Status
RUO
Other Names
IL-7 receptor α chain, IL-7Rα
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
A019D5_Pure_021111
Human peripheral blood lymphocytes were stained with purified CD127 (clone A019D5) (filled histogram) or mouse IgG1 isotype control (open histogram), followed by anti-mouse IgG FITC.
  • A019D5_Pure_021111
    Human peripheral blood lymphocytes were stained with purified CD127 (clone A019D5) (filled histogram) or mouse IgG1 isotype control (open histogram), followed by anti-mouse IgG FITC.
Cat # Size Price Quantity Check Availability Save
351302 100 µg 100€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage cells, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be a useful marker for identifying memory and effector T cells. Studies have shown that CD127 expression is down-modulated on Treg cells. It can be used as a marker for differentiation of Treg and conventional T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cell proliferation and development.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CD127
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
PG - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported (for the relevant formats) application: proteogenomics1.

Application References
  1. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Lundtoft C, et al. 2017. PLoS Pathogens. 13(6):e1006425. PubMed
  2. Alroqi F, et al. 2017. J Clin Immunol. . 10.1007/s10875-017-0451-1. PubMed
  3. Fu J et al. 2019. Cell stem cell. 24(2):227-239 . PubMed
  4. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
  5. Cardoso V, et al. 2017. Nature. 549:277. PubMed
  6. Yoshihara S, et al. 2019. Front Immunol. 0.545833333. PubMed
  7. Fan J, et al. 2019. Nat Commun. 1.984027778. PubMed
  8. Abdel-Gadir A, et al. 2019. Nat Med. 25:1164. PubMed
  9. Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed
  10. Li Y, et al. 2019. Front Immunol. 0.460416667. PubMed
  11. Tripathi T, et al. 2019. Immunohorizons. 0.201388889. PubMed
  12. Michlmayr D, et al. 2020. Cell Reports. 31(4):107569. PubMed
  13. Chng MHY, et al. 2020. Immunity. 51(6):1119-1135.e5.. PubMed
  14. Mousset CM, et al. 2018. Oncoimmunology. 7:e1488565. PubMed
  15. Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
  16. Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
  17. Mishra A, et al. 2021. Cell. 184(13):3394-3409.e20. PubMed
  18. Schwabenland M, et al. 2021. Immunity. . PubMed
  19. Rouers A, et al. 2021. Cell Rep Med. 2:100278. PubMed
  20. Oba T, et al. 2021. J Immunother Cancer. 9:. PubMed
  21. NULL, et al. 2022. Cell. 185:916. PubMed
  22. Umeda M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  23. Cobb DA, et al. 2022. J Immunother Cancer. 10:. PubMed
  24. Kondo H, et al. 2022. Front Immunol. 13:836923. PubMed
  25. Spoerl S, et al. 2021. Eur J Immunol. 51:1436. PubMed
  26. Alpert A, et al. 2022. Cell Syst. 13:71. PubMed
  27. Leader AM, et al. 2021. Cancer Cell. 39:1594. PubMed
  28. Glass MC, et al. 2022. Cell Rep. 39:110728. PubMed
  29. McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed
  30. Poon MML, et al. 2023. Nat Immunol. 24:309. PubMed
RRID
AB_10718513 (BioLegend Cat. No. 351302)

Antigen Details

Structure
Type I transmembrane glycoprotein, associates with CD132, 60-90 kD
Distribution

Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells

Function
T cell and immature B cell proliferation and development
Ligand/Receptor
IL-7
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. He YW and Malek TR. 1998. Crit. Rev. Immunol. 18:503.
3. Huster KM, et al. 2004. P. Natl. Acad. Sci. USA 101:5610.
4. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.
5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707.
6. Liu W, et al. 2006. J. Exp. Med. 203:1701.

Gene ID
3575 View all products for this Gene ID
UniProt
View information about CD127 on UniProt.org
Go To Top Version: 2    Revision Date: 08.15.2022

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account