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Purified anti-human CD14 Antibody

Pricing & Availability
Clone
M5E2 (See other available formats)
Regulatory Status
RUO
Workshop
III 329
Other Names
LPS receptor
Isotype
Mouse IgG2a, κ
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Product Citations
publications
M5E2
Human peripheral blood monocytes stained with M5E2 APC
  • M5E2
    Human peripheral blood monocytes stained with M5E2 APC
Cat # Size Price Quantity Check Availability Save
301801 25 µg 34€
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301802 100 µg 72€
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Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens, and in the upregulation of adhesion molecules and expression of cytokines in monocytes and neutrophils.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length human CD14 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
Block, IHC-F, PG - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation4, and immunofluorescence microscopy5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301861 and 301862).

Application References
  1. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  2. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (IHC-F)
  3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  4. Power CP, et al. 2004. J. Immunol. 173:5229. (Block)
  5. Williams KC, et al. 2001. J. Exp. Med. 193:905.
  6. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (FC) PubMed
  7. Santer DM, et al. 2010. J. Immunol. 485:4739. PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Zizzo G, et al. 2012. J. Immunol. 189:3508. PubMed
  10. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  11. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Winkler M, et al. 2017. PLoS One.. 10.1371/journal.pone.0182427. PubMed
  2. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  3. Olin A, et al. 2018. Cell. 174:1277. PubMed
  4. Zimmerman MG, et al. 2018. Cell Host Microbe. 24:731. PubMed
  5. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  6. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
  7. Zhao J, et al. 2017. Sci Immunol. 2:eaan5400. PubMed
  8. Cao Q, et al. 2018. Am J Physiol Renal Physiol. 314:F561. PubMed
  9. Ortiz AM, et al. 2018. Nat Med. 24:1313. PubMed
  10. Agrawal N, et al. 2018. Front Immunol. 2.053472222. PubMed
  11. Humblet–Baron S, et al. 2019. J Allergy Clin Immunol. 143:2215. PubMed
  12. Nguyen LT, et al. 2019. Cancer Immunol Immunother. 68:773. PubMed
  13. Schaut RG, et al. 2019. Sci Rep. 4.820138889. PubMed
  14. Tian Y, et al. 2019. Cell Rep. 29:4482. PubMed
  15. Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
  16. Melo-Gonzalez F, et al. 2018. J Biol Chem. 293:8543. PubMed
  17. Auladell M, et al. 2019. Clin Transl Immunology. 8:e01090. PubMed
  18. Oda H, et al. 2019. Front Immunol. 10:479. PubMed
  19. Kong R, et al. 2019. Cell. 178:567. PubMed
  20. Lakshmikanth T, et al. 2020. Cell Reports. 32(3):107923. PubMed
  21. Sung JA, et al. 2018. Mol Ther. 26:2496. PubMed
  22. Iwamoto S, et al. 2007. J Immunol. 179:1449. PubMed
  23. Zizzo G, et al. 2012. J Immunol. 189:3508. PubMed
  24. Tal MC, et al. 2020. MBio. 11:00. PubMed
  25. Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
  26. Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
  27. Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
  28. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
  29. Mishra A, et al. 2021. Cell. 184(13):3394-3409.e20. PubMed
  30. Henrick BM, et al. 2021. Cell. . PubMed
  31. Schwabenland M, et al. 2021. Immunity. . PubMed
  32. Wastyk HC, et al. 2021. Cell. 184(16):4137-4153.e14. PubMed
  33. Martin E, et al. 2020. JCI Insight. :5. PubMed
  34. De Maeyer RPH, et al. 2020. Nat Immunol. 21:615. PubMed
  35. Chiou SH, et al. 2021. Immunity. 54:586. PubMed
  36. Raffray L, et al. 2020. Immunology. 159:88. PubMed
  37. Li H, et al. 2020. Front Immunol. 11:1777. PubMed
  38. Korn MA, et al. 2020. J Immunol. 205:2595. PubMed
  39. Ehinger E, et al. 2021. Cardiovasc Res. 117:1166. PubMed
  40. Gao H, et al. 2021. J Virol. :. PubMed
  41. Gadalla R, et al. 2022. STAR Protoc. 3:101643. PubMed
  42. Roberts JT, et al. 2020. Mol Cell Proteomics. 19:362. PubMed
  43. Sattler A, et al. 2021. Am J Transplant. 21:87. PubMed
  44. Coelho CH, et al. 2020. JCI Insight. 5:. PubMed
  45. Alhaj Hussen K, et al. 2020. Front Immunol. 11:579776. PubMed
  46. Kaushal A, et al. 2021. Blood Cancer Discov. 2:600. PubMed
  47. Baskar R, et al. 2022. Cell Rep Methods. 2:. PubMed
  48. Alpert A, et al. 2022. Cell Syst. 13:71. PubMed
  49. McIlwain DR, et al. 2021. Cell Host Microbe. 29:1828. PubMed
  50. Glass MC, et al. 2022. Cell Rep. 39:110728. PubMed
  51. McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed
  52. Feyaerts D, et al. 2022. Cell Rep Med. 3:100680. PubMed
RRID
AB_314183 (BioLegend Cat. No. 301801)
AB_314184 (BioLegend Cat. No. 301802)

Antigen Details

Structure
GPI-linked membrane glycoprotein, 53-55 kD
Distribution

Monocytes, macrophages, granulocytes (low)

Function
LPS receptor, clearance of Gram-negative pathogens
Ligand/Receptor
LPS
Cell Type
Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.

Gene ID
929 View all products for this Gene ID
UniProt
View information about CD14 on UniProt.org
Go To Top Version: 5    Revision Date: 05.14.2018

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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