- Clone
- 3A6 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- HCDM listed
- Other Names
- CD6 ligand, Activated Leukocyte Cell Adhesion Molecule (ALCAM)
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A_3A6_PE_082509 A_3A6_PE_082509](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A_3A6_PE_082109.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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PHA-stimulated human peripheral blood lymphocytes (3 days) stained with purified 3A6 conjugated to PE -
Human peripheral blood monocytes stained with purified 3A6 conjugated to PE
Cat # | Size | Price | Quantity Check Availability | Save | ||
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343902 | 100 µg | 104€ |
CD166, also known as the CD6 ligand or the Activated Leukocyte Cell Adhesion Molecule (ALCAM), is a 100-105 kD transmembrane glycoprotein. It belongs to the Ig superfamily of proteins and expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, and neurons. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently CD166 has also been used as a potential cancer stem cell marker. The antibody reacts with human activated leukocyte cell adhesion molecule (ALCAM).
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Cultured human thymic epithelial cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
IHC-P, ICC - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections and immunofluorescence.1
- Application References
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- Pretzel D, et al. 2011. Arthritis Res. Ther. 13:R64. (IHC, IF, FC)
- Product Citations
- RRID
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AB_2223892 (BioLegend Cat. No. 343902)
Antigen Details
- Structure
- A type I transmembrane glycoprotein belonging to the Ig superfamily of proteins.
- Distribution
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CD166 is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, and neurons.
- Function
- Play an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development.
- Ligand/Receptor
- CD6
- Cell Type
- Epithelial cells, Fibroblasts, Mesenchymal Stem Cells, Monocytes, Neurons, T cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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1. Aruffo A, et al. 1997. Immunol Today. 18(10):498
2. Patel DD, et al. 1995. J. Exp. Med. 181:2213
3. Bowen MA, et al. 1995. J. Exp. Med. 181:1563
4. Horst D, et al. 2009. Cancer Invest. 22:1 - Gene ID
- 214 View all products for this Gene ID
- UniProt
- View information about CD166 on UniProt.org
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