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Purified anti-human CD34 Antibody

Pricing & Availability
Clone
581 (See other available formats)
Regulatory Status
RUO
Workshop
V MA27
Other Names
Gp105-120, My10
Isotype
Mouse IgG1, κ
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Product Citations
publications
581_Pure_030510
Human myeloid cell line KG1a stained with purified 581, followed by anti-mouse IgG FITC
  • 581_Pure_030510
    Human myeloid cell line KG1a stained with purified 581, followed by anti-mouse IgG FITC
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343501 25 µg 48€
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343502 100 µg 92€
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Description

CD34, also known as gp105-120, is a type I monomeric sialomucin-like glycophosphoprotein with an approximate molecular weight of 105-120 kD. Selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue, CD34 is a commonly used marker to identify human hematopoietic stem/progenitor cells. According to the differential sensitivity to enzymatic cleavage, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
ICC, IHC-P - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The 581 antibody recognizes the class III group epitope which is resistant to sialidase/glycolyprotease and chymopapain treatment. Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections5 and immunofluorescence6.

Application References
  1. Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigen. New York:Oxford University Press.
  2. Felschow DM, et al. 2001. Blood 97:3768.
  3. Rudin CE, et al. 1997. Br. J. Haematol. 97:488.
  4. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  5. Skowasch D, et al. 2003. Cardiovasc Res. 60:684. (IHC)
  6. Umland O, et al. 2003. J. Histochem. Cytochem. 51:977. (IF)
Product Citations
  1. Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed
  2. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  3. Zhou W et al. 2017. Cell stem cell. 21(5):591-603 . PubMed
  4. Ran LJ, et al. 2018. Mol Med Rep. 18:723. PubMed
  5. Wei SC et al. 2017. Cell. 170(6):1120-1133 . PubMed
  6. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
  7. Henrick BM, et al. 2021. Cell. . PubMed
  8. Richmond BW, et al. 2020. Mucosal Immunol. . PubMed
  9. Crawford LB, et al. 2021. J Virol. 95:. PubMed
  10. Kato M, et al. 2021. PLoS One. 16:e0252116. PubMed
  11. Liu C, et al. 2022. Regen Ther. 21:192. PubMed
  12. Alpert A, et al. 2022. Cell Syst. 13:71. PubMed
  13. Zhang A, et al. 2022. EMBO J. 41:e109187. PubMed
RRID
AB_1731969 (BioLegend Cat. No. 343501)
AB_1731898 (BioLegend Cat. No. 343502)

Antigen Details

Structure
105-120 kD single chain mucin-like glycoprotein
Distribution

Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts

Function
Cell adhesion
Ligand/Receptor
L-selectin, E-selectin
Cell Type
Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Krause DS, et al. 1996. Blood 87:1.
2. Puri KD, et al. 1995. J. Cell Biol. 131:261.
3. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. John Wiley & Sons Inc, Hoboken New Jersey.

Gene ID
947 View all products for this Gene ID
UniProt
View information about CD34 on UniProt.org
Go To Top Version: 3    Revision Date: 08.23.2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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