- Clone
- M1606B04 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Mac-2, Gal-3, RL-29, galactose-specific lectin 3, CBP-35, L-34, εBP
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![M1606B04_PURE_Mac-2_Galectin-3_Antibody_WB_062918 M1606B04_PURE_Mac-2_Galectin-3_Antibody_WB_062918](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/M1606B04_PURE_Mac-2_Galectin-3_Antibody_WB_062918.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Cell lysate from HepG2 (lane 1), A549 (lane 2), PC3 (lane 3), HaCaT (lane 4), MCF-7 (lane 5), HeLa (lane 6) and NIH3T3 cell lines (lane 7) (15 µg protein for each) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL (1:500 dilution) of purified anti-Mac-2 (Galectin-3) antibody (clone M1606B04) (upper). Proteins were visualized using chemiluminescence detection after incubation with a 1:3000 dilution of HRP conjugated anti-rat-IgG secondary antibody for the anti-Mac-2 (Galectin-3) antibody (upper panel) or a 1:5000 dilution of Direct-Blot™ HRP anti-β-Actin (clone 2F1-1, lower panel) antibody. Lane M: Molecular weight ladder.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
677301 | 50 µg | 57€ | ||||
677302 | 500 µg | 207€ |
Galectin-3 (galactose-specific lectin 3) is a unique member of the β-galactoside-binding lectin family molecule, which plays an important role in regulation of cell proliferation, differentiation, and tumor cell metastasis. Galectin-3 is mainly found in the cytoplasm, but it is also seen in the nucleus and can be secreted by the non-classical, secretory pathway. In general, secreted galectin-3 mediates cell migration, cell adhesion, and cell-cell interactions through its high affinity binding with galactose-containing glycoproteins on the cell surface. Cytoplasmic galectin-3 exhibits anti-apoptotic activity and regulates several signal transduction pathways, whereas nuclear galectin-3 has been associated with pre-mRNA splicing and gene expression. Recent studies also indicate the potential role of galectin-3 as a biomarker for acute and chronic heart failure.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant full-length human Galectin-3.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IP - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 1-5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
- RRID
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AB_2565901 (BioLegend Cat. No. 677301)
AB_2565902 (BioLegend Cat. No. 677302)
Antigen Details
- Structure
- 250 amino acids with a predicted molecular weight of approximately 26 kD.
- Distribution
-
Localized in the nucleus, cytoplasm, cell surface, or extracellular matrix. Expressed by tumor cells, monocytes/macrophages, epithelial cells, Kupffer cells, and dendritic cells.
- Function
- Regulate cell proliferation, differentiation, adhesion, and anti-apoptosis.
- Interaction
- DMBT1, ITGA3, ITGB1, CSPG4, LGALS3BP, LYPD3, CYHR1, and UACA.
- Ligand/Receptor
- Variety of ligands such as IgE, IgA, galactose, casein kinase I, laminin, mucin, LAMPs, CD66, and etc.
- Cell Type
- Dendritic cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Immunology, Signal Transduction, Transcription Factors
- Molecular Family
- Adhesion Molecules
- Antigen References
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1. Kim HR, et al. 1999. Cancer Res. 16:4148.
2. Reljic R, et al. 2004. Immunol. Lett. 93:51.
3. Takenaka Y, et al. 2004. Mol. and Cell. Biol. 24:4395.
4. Joo HG, et al. 2001. J Leuko. Biol. 4:555.
5. Gudowska M, et al. 2014. Pol. Merkur. Lekarski. 37:348.
6. Xin M, et al. 2015. Biomed. Pharmacother. 69:179.
7. Filipe MD, et al. 2015. Clin. Chim. Acta. 443:48.
8. Funasaka T, et al. 2014. Glycobiology 24:886. - Gene ID
- 3958 View all products for this Gene ID
- UniProt
- View information about Mac-2 on UniProt.org
Related Pages & Pathways
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