- Clone
- 20/70 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- C5a ligand, C5a anaphylatoxin receptor, C5AR, C5R1, C5AR1
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![20-70_ULEAF_CD88_Antibody_112619.png 20-70_ULEAF_CD88_Antibody_112619.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/20-70_ULEAF_CD88_Antibody_112619.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse bone marrow granulocytes stained with Ly-6G/Ly-6C (Gr-1) APC and CD88 (C5aR) (clone 20/70) purified (left) or rat IgG2b, κ purified isotype ctrl (right) followed by anti-rat IgG FITC.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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135802 | 100 µg | 118€ |
CD88 is a single chain protein with seven membrane-spanning regions and a molecular weight of 43 kD. CD88 is expressed by neutrophils, eosinophils, and monocytes. There have been reports of CD88 expression in non-immune cells such as glial cells, cerebellar granule cells, cardiomyocytes, and vascular endothelial cells. CD88 is coupled to heterotrimeric G proteins such as Gi, and after C5a binding, the signal transduced by CD88 results in the activation of PLCβ, PI-3 kinase, and PLA2, among other molecules, resulting in chemotaxis, granule enzyme release, and superoxide anion production.
Product DetailsProduct Details
- Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- mC5aR transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications for the relevant formats include: blocking of C5a binding.
- Application References
-
- Godau J, et al. 2004. J. Immunol. 173:3437.
- Shushakova N, et al. 2002. J. Clin. Invest. 110:1823.
- Kim SH, et al. 2013. Int Immunol. 25:623. PubMed
- Product Citations
- RRID
-
AB_1953295 (BioLegend Cat. No. 135802)
Antigen Details
- Structure
- Single chain protein with seven membrane-spanning regions, 43 kD.
- Distribution
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Neutrophils, eosinophils, monocytes, glial cells, cerebellar granule cells, cardiomyocytes, and vascular endothelial cells.
- Function
- After C5a binding, the CD88 signal results in chemotaxis, granule enzyme release and superoxide anion production.
- Interaction
- CD88 is coupled to heterotrimeric G proteins such as Gi, The signal transduced by CD88 results in the activation of PLCβ, PI-3 kinase, and PLA2, among other molecules.
- Bioactivity
- Chemotaxis, degranulation.
- Cell Type
- Dendritic cells, Endothelial cells, Eosinophils, Monocytes, Neutrophils
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
-
1. Schraufstatter IU, et al. 2009. J. Immunol. 182:3827.
2. Ippel JH, et al. 2009. J. Biol. Chem. 284:12363.
3. Monk PN, et al. 2007. Br. J. Pharmacol. 152:429.
4. Griffin RS, et al. 2007. J. Neurosci. 27:8699.
5. Lee H, et al. 2006. Nat. Biotechnol. 24:1279.
6. Buck E and Wells JA, et al. 2005. P. Natl. Acad. Sci. USA 102:2719. - Gene ID
- 12273 View all products for this Gene ID
- UniProt
- View information about CD88 on UniProt.org
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