- Clone
- M7004D06 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Microtubule associated protein tau
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![M7004D06_PURE_TauPhospho_Antibody_1_041618 M7004D06_PURE_TauPhospho_Antibody_1_041618](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/M7004D06_PURE_TauPhospho_Antibody_1_041618.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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IHC staining of anti-Tau Phospho (Thr181) antibody (clone M7004D06) on formalin-fixed paraffin-embedded Alzheimer’s brain tissue, and lack of staining of normal brain tissue. Following antigen retrieval using Sodium Citrate, the tissue was incubated with the primary antibody at 10 µg/ml for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit was used for detection. -
IHC staining of purified anti-Tau phospho (Thr181) antibody (clone M7004D06) on formalin-fixed paraffin-embedded Alzheimer's disease brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5 µg/ml of the primary antibody for one hour at room temperature, followed by incubation with Alexa Fluor® 594 Goat anti-mouse IgG (Cat. No. 405326) for one hour at room temperature. Nuclei were counterstained with Hoechst stain. The image was captured by 40X objective. Scale bar: 50 µm -
Western blot of anti-Tau Phospho (Thr181) (clone M7004D06), anti-Tau Phospho (Ser396) (clone PHF-13) antibodies, and isotype-matched control IgG1 and IgG2b. Lane 1: 20 µg of Parkinson’s disease human brain lysate; Lane 2: 2 µg of Tau paired helical filaments. Blots were incubated overnight at 4°C with 10 µg/ml of the primary antibody, followed by horseradish peroxidase labeled goat anti-mouse secondary antibody. Western blot of anti-Tau Phospho (Ser396) (clone PHF-13) serves as a positive control for Tau staining. -
Direct ELISA of purified anti-Tau Phospho (Thr181) antibody (clone M7004D06) binding to plate-immobilized human phospho-tau (Thr181) peptide and scrambled sequence control peptide. ELISA was performed by coating wells with 150 ng of phospho-tau or scrambled peptides. The wells were then incubated with the primary antibody at 37°C for 45 minutes, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) was used as the detection system.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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846601 | 25 µg | 90€ | ||||
846602 | 100 µg | 240€ |
Tau protein promotes microtubule assembly and stability. Tau is abundant in neurons of the central nervous system, and is expressed at low levels in astrocytes and oligodendrocytes. Abnormal hyper-phosphorylation, aggregation, and toxic gain of function of tau is associated with several neurological disorders, including Alzheimer’s disease (AD). The major building block of neurofibrillary lesions in AD brains consists of paired helical filaments (PHFs) of abnormally hyperphosphorylated tau. Recent studies indicate that cerebrospinal fluid tau phosphorylated at position threonine 181 has diagnostic utility for several neurological disorders. Six isoforms of tau are generated by alternative splicing of the MAPT gene. These isoforms are distinguished by the number of tubulin binding domains, 3 (3R) or 4 (4R), in the C-terminal of the protein and by one (1N), two (2N), or no (0N) inserts in the N-terminal domain. Tau isoforms are differentially expressed during development.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- 15-mer pT181 Tau peptide conjugated to KLH.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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IHC-P - Quality tested
WB, Direct ELISA - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 μg/ml is suggested. For Western blotting, a concentration range of 2.0 - 10 μg/ml is suggested. For Direct ELISA, a concentration range of 1.0 - 10 μg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
- RRID
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AB_2715859 (BioLegend Cat. No. 846601)
AB_2616881 (BioLegend Cat. No. 846602)
Antigen Details
- Structure
- Unmodified Tau isoforms have an apparent molecular weight ranging from 33-79 kD. Additional high and low molecular weight Tau species have been observed in brain tissues.
- Distribution
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Tissue distribution: central nervous system, peripheral ganglia and nerves, kidney, skeletal, and heart muscle.
Cellular distribution: cytoskeleton, nucleus, plasma membrane, and cytosol. - Function
- Tau promotes microtubule assembly and stability. The short tau isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
- Interaction
- Tau interacts with Sequestosome-1, Peptidyl-prolyl cis-trans isomerase FKBP4, Casein kinase I isoform delta, Serine/threonine-protein kinase Sgk1, Laforin, Alpha-synuclein
- Molecular Family
- Phospho-Proteins, Tau
- Antigen References
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1. Meredith JE Jr, et al. 2013. PLoS One 8(10):e76523. PubMed
2. Goodall CA, et al. 2006. J. Neurol. Neurosurg. Psychiatry 77(1):89. PubMed - Gene ID
- 4137 View all products for this Gene ID
- UniProt
- View information about Tau Phospho Thr181 on UniProt.org
Related Pages & Pathways
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