Recombinant Human IL-2 ELISA Std.

Pricing & Availability

Regulatory Status: RUO
Other Names: Interleukin-2, T cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF)
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Product Citations: publications

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570409

4 pack

76€

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Description

IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells promoting proliferation and maturation. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.

Product Details

Technical Data Sheet (pdf)

Product Details

Source

Human IL-2, Ala 21 - Thr 153 - Accession Number NM_000586, was expressed in E. coli.

Molecular Mass

The 133 amino acid recombinant protein has a predicted molecular mass of 15,418 Da. The DTT-reduced and non-reduced protein migrates at approximately 13kDa by SDS-PAGE. The N-terminal amino acid is Ala.

Purity

Purity is >98% as determined by SDS PAGE of 6 µg of reduced and non-reduced recombinant human IL-2.

Formulation

Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

Unopened vials can be stored between 2°C and 8°C until the expiration date. Prior to use, reconstitute the lyophilized powder with 0.2 mL of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Avoid repeated freeze/thaw cycles.

Application

ELISA

Recommended Usage

Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 2000 pg/ml to 15 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This IL-2 protein is useful as a standard for a human IL-2 sandwich ELISA, using unlabeled MQ1-17H12 antibody (catalog #500302) for capture and biotinylated Poly5176 antibody (catalog #517605) for detection.

Product Citations

Strazza M, et al. 2022. Mol Ther Methods Clin Dev. 27:380. PubMed

Antigen Details

Structure: Cytokine
Bioactivity: Proliferation of T lymphocytes, B cells, anti-inflammatory, hematopoiesis, tumor surveillance
Cell Sources: T cells
Cell Targets: T cells, B cells, NK cells, LAK cells, monocytes, macrophages, oligodendrocytes
Receptors: High affinity heterotrimer of IL-2Rα/β/γ, intermediate affinity homodimer IL-2Rα (CD25; p55; Tac) and heterodimer IL-2Rβ (CD122)/γ; γ-subunit (CD132) in common with IL-4R, IL-7R, IL-13R, IL-15R
Biology Area: Stem Cells, Cell Biology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Taniguchi T, et al. 1993. Cell 73:5.
3. Nistico G. 1993. Prog. Neurobiol. 40:463.
4. Waldmann T, et al. 1993. Ann. NY Acad. Sci. 685:603.
Regulation: Upregulated by NFAT; downregulated by TCF-8, CIF (colostrum inhibitory factor)
Gene ID: 3558 View all products for this Gene ID
UniProt: View information about IL-2 on UniProt.org