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APC anti-mouse NK-1.1 Antibody

Pricing & Availability
Clone
PK136 (See other available formats)
Regulatory Status
RUO
Other Names
NKR-P1C, NKR-P1B, Ly-55, CD161, CD161b, CD161c
Isotype
Mouse IgG2a, κ
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Product Citations
publications
PK136_APC_1_110210
C57BL/6 mouse splenocytes were stained with CD49b (DX5) FITC and NK1.1 (clone PK136) APC (top) or mouse IgG2a APC isotype control (bottom).
  • PK136_APC_1_110210
    C57BL/6 mouse splenocytes were stained with CD49b (DX5) FITC and NK1.1 (clone PK136) APC (top) or mouse IgG2a APC isotype control (bottom).
  • PK136_APC_2_110210
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
108709 25 µg 71€
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108710 100 µg 234€
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Description

NK-1.1 surface antigen, also known as CD161b/CD161c and Ly-55, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NK-1+ cells from mouse spleen and bone marrow
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The NK-1.1 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, complement-dependent cytotoxicity3, in vivo depletion4,5,9,10, mediation of in vitro redirected lysis6, blocking of NK cell function7, induction of proliferation8, immunohistochemical staining of frozen sections11, immunofluorescence microscopy11, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 108712).

Application References
  1. Carlyle JR, et al. 1999. J. Immunol. 162:5917. (IP)
  2. Sentman CL, et al. 1989. Hybridoma 8:605. (IP)
  3. Koo GC, et al. 1984. Hybridoma 3:301. (Cyt)
  4. Sentman CL, et al. 1989. J. Immunol. 142:1847. (Deplete)
  5. Koo GC, et al. 1986. J. Immunol. 137:3742. (Deplete)
  6. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  7. Kung SK, et al. 1999. J. Immunol. 162:5876. (Block)
  8. Reichlin A, et al. 1998. Immunol. Cell Biol. 76:143.
  9. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  10. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (Deplete)
  11. Kanwar JR, et al. 2001. J. Natl. Cancer Inst. 93:1541. (IHC, IF)
  12. Kroemer A, et al. 2008. J. Immunol. 180:7818. PubMed
  13. Kim JY, et al. 2009. Exp Mol Med. 30:288. PubMed
  14. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  15. Lee H, et al. 2014. Invest Ophthalmol Vis Sci. 55:2885. PubMed
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Zhao D, et al. 2021. Innate Immun. 27:533. PubMed
  2. Hutter K, et al. 2022. Front Immunol. 13:967914. PubMed
  3. Furrer R, et al. 2021. Sci Adv. 7:eabi4852. PubMed
  4. Zhou Y, et al. 2021. Methods Mol Biol. 2388:35. PubMed
  5. Xin B, et al. 2022. Hepatology. 76:630. PubMed
  6. Zhang H, et al. 2022. Eur J Immunol. 52:978. PubMed
  7. Brog RA, et al. 2022. Cancer Immunol Res. 10:962. PubMed
  8. Liu W, et al. 2022. Biomedicines. 10: . PubMed
  9. Simpson AP, et al. 2022. Cell Rep. 40:111099. PubMed
  10. Hao J, et al. 2022. Cell Rep. 41:111804. PubMed
  11. Jin SM, et al. 2023. Nat Nanotechnol. :. PubMed
  12. Zhu X, et al. 2023. Front Immunol. 14:1114802. PubMed
  13. Kuehm LM, et al. 2021. Cancer Immunol Res. 9:227. PubMed
  14. Leick MB, et al. 2022. Cancer Cell. 40:494. PubMed
  15. Wight AE, et al. 2022. Proc Natl Acad Sci U S A. 119:e2200757119. PubMed
  16. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  17. Yu X, et al. 2020. Nat Commun. 11:1110. PubMed
  18. Pi C, et al. 2022. Cancers (Basel). 14:. PubMed
  19. Schroeter CB, et al. 2022. J Neuroinflammation. 19:270. PubMed
  20. Gao L, et al. 2023. J Nanobiotechnology. 21:56. PubMed
  21. Roussel-Queval A, et al. 2023. STAR Protoc. 4:102119. PubMed
  22. Wang YC, et al. 2023. J Exp Med. 220:. PubMed
  23. Wang JX, et al. 2023. JCI Insight. 8:. PubMed
  24. Yin C, et al. 2023. Sci Rep. 13:3903. PubMed
  25. van Vloten JP, et al. 2022. J Immunother Cancer. 10:. PubMed
  26. Symeonidou V, et al. 2021. Cell Rep. 37:109900. PubMed
  27. Afolabi LO, et al. 2021. Front Immunol. 12:701671. PubMed
  28. Liu W, et al. 2020. Mol Ther Oncolytics. 17:350. PubMed
  29. Alissafi T, et al. 2018. J Clin Invest. 128:3840. PubMed
  30. Zhang J, et al. 2021. MedComm (Beijing). 2:256. PubMed
  31. Stegelmeier AA, et al. 2022. Biomedicines. 10:. PubMed
  32. Wilson RP, et al. 2019. PLoS Pathog. 15:e1007745. PubMed
  33. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  34. Shen T, et al. 2019. Cell Death Discov. 5:123. PubMed
  35. Mirshahi F, et al. 2022. Cell Rep. 38:110454. PubMed
  36. Sun SJ, et al. 2022. Commun Biol. 5:867. PubMed
  37. Bangs DJ, et al. 2022. Cell Rep. 38:110266. PubMed
  38. Liang Y, et al. 2020. J Immunol. 204:2984. PubMed
  39. Chen WS, et al. 2021. Cell Rep. 37:109974. PubMed
  40. Zhao J, et al. 2019. Nat Commun. 10:899. PubMed
  41. Neumann AM et al. 2017. PloS one. 12(11):e0187330 . PubMed
  42. Shi S, et al. 2020. Clin Cancer Res. 26:5990. PubMed
  43. Papafragkos I, et al. 2022. Front Immunol. 13:889075. PubMed
  44. Hoffman D, et al. 2021. Immunity. 54:2712. PubMed
  45. Ohtsuki T, et al. 2016. J Virol. 90: 300 - 307. PubMed
  46. Starkl P, et al. 2020. Immunity. 53(4):793-804.e9. PubMed
  47. Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
  48. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  49. Bourn JR, et al. 2021. Cancer Lett. 503:75. PubMed
  50. Janakiram N, et al. 2016. Sci Rep. 6:37046. PubMed
  51. Pan W et al. 2017. Immunity. 47(2):284-297 . PubMed
  52. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  53. Hirata Y, et al. 2015. PLoS One. 10: 0141785. PubMed
  54. Wei SC, et al. 2020. Cancer Discov. . PubMed
  55. Dos Santos Dias L, et al. 2021. PLoS Pathog. e1009324:17. PubMed
  56. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  57. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
  58. Guo L, et al. 2021. Front Immunol. 12:785457. PubMed
  59. Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed
  60. Kotfis K, et al. 2020. British Journal of Pharmacology. 49(1):66-72. PubMed
  61. Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed
  62. Hernández–Santos N, et al. 2018. Cell Host Microbe. 1.313194444. PubMed
  63. Progatzky F, et al. 2021. Nature. 599:125. PubMed
  64. Zenker S, et al. 2014. J Immunol. 192:2830. PubMed
  65. Ray A, et al. 2015. PLoS One. 10: 0137314. PubMed
  66. Satoh M, et al. 2016. Sci Rep. 6:28473. PubMed
  67. Lehmann M, et al. 2016. J Leukoc Biol. 99: 1057 - 1064. PubMed
  68. Hutter K, et al. 2020. FEBS J. . PubMed
  69. Xu J et al. 2018. Cell. 173(3):762-775 . PubMed
  70. Zhang Y, et al. 2021. J Immunol Res. 2021:5599439. PubMed
  71. Stotesbury C, et al. 2020. J Immunol. 204:1582. PubMed
  72. Textor A, et al. 2014. Cancer Res. 74:6769. PubMed
  73. Bourque J, et al. 2021. Heliyon. 7:e08311. PubMed
  74. Kim S, et al. 2021. Elife. 10:. PubMed
  75. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  76. Puigdelloses M, et al. 2021. J Immunother Cancer. 9:. PubMed
  77. Dobson HE, et al. 2020. Mucosal Immunol. 0.901388889. PubMed
  78. Lim S, et al. 2016. PLoS One. 11: 0155689. PubMed
  79. Solaymani-Mohammadi S, et al. 2016. J Leukoc Biol. 99: 475 - 482. PubMed
  80. Ballet R, et al. 2014. PLoS Pathog. 10:1004550. PubMed
  81. Stifter SA, et al. 2019. Cell Rep. 29:3539. PubMed
  82. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  83. Yu X, et al. 2019. Nat Commun. 10:574. PubMed
  84. Tuong ZK, et al. 2021. iScience. 24:103326. PubMed
  85. Yu H, et al. 2021. Front Oncol. 11:736882. PubMed
  86. Wüthrich M, et al. 2021. MBio. 12:e0201821. PubMed
  87. Kohn EM, et al. 2022. J Immunol. 208:1417. PubMed
  88. Zhang P, et al. 2022. Nat Commun. 13:1588. PubMed
  89. Terhorst D, et al. 2015. J Immunol. 195: 4953 - 4961. PubMed
  90. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  91. DiToro D, et al. 2020. Immunity. 650:52. PubMed
  92. Nahrendorf W, et al. 2021. eLife. 10:00. PubMed
  93. Tsuzuki S, et al. 2015. Int Immunol. 10.1093/intimm/dxv058. PubMed
  94. Burgener SS, et al. 2019. Cell Rep. 27:3646. PubMed
  95. Dhar P, et al. 2021. Commun Biol. 4:905. PubMed
  96. Cartwright ANR, et al. 2021. Cancer Immunol Res. 9:470. PubMed
  97. Woodring T, et al. 2022. iScience. 25:104934. PubMed
  98. Zhang H, et al. 2017. Immunol Cell Biol. 10.1038/icb.2017.11. PubMed
  99. Schuler F, et al. 2017. Nat Commun. . 10.1038/s41467-017-01850-4. PubMed
  100. Xu JL, et al. 2022. Front Pharmacol. 13:911663. PubMed
  101. Huang W, et al. 2014. J Leukoc Biol. 96:55. PubMed
  102. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  103. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
  104. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  105. Peng V, et al. 2022. Nat Immunol. 23:619. PubMed
  106. Stotesbury C, et al. 2020. Aging Cell. 19:e13170. PubMed
  107. Tomita T, et al. 2021. Nat Commun. 12:3655. PubMed
  108. Bonavita E, et al. 2020. Immunity. 1215:53. PubMed
  109. Pelly V, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.4. PubMed
  110. Fleskens V, et al. 2019. Cell Rep. 26:3600. PubMed
  111. Kujur W, et al. 2020. PLoS Pathog. 16:e1009132. PubMed
  112. Gawish R, et al. 2022. Elife. 11:. PubMed
  113. Zhang S, et al. 2016. PLoS Pathog. 12: 1005617. PubMed
  114. Liu CF, et al. 2017. Front Immunol. 1.445833333. PubMed
  115. Schmidts A, et al. 2020. J Immunother Cancer. 8:. PubMed
  116. Hester AK, et al. 2022. Cell Rep. 38:110363. PubMed
  117. Song X, et al. 2022. Transl Oncol. 15:101306. PubMed
  118. Nabekura T, et al. 2020. Immunity. 96:52. PubMed
RRID
AB_313396 (BioLegend Cat. No. 108709)
AB_313397 (BioLegend Cat. No. 108710)

Antigen Details

Structure
NKR-P1 gene family
Distribution

NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB/N, NZB)

Function
NK cell activation, IFN-γ production, cytotoxic granule release
Cell Type
NK cells, NKT cells
Biology Area
Immunology, Innate Immunity
Antigen References

1. Lanier LL. 1997. Immunity 6:371.
2. Yokoyama WM, et al. 1993. Ann. Rev. Immunol. 11:613.
3. Koo GC, et al. 1986. J. Immunol. 137:3742.
4. Giorda R, et al. 1991. J. Immunol. 147:1701.

Gene ID
17059 View all products for this Gene ID
UniProt
View information about NK-1.1 on UniProt.org

Other Formats

View All NK-1.1 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse NK-1.1 PK136 FC
Biotin anti-mouse NK-1.1 PK136 FC
FITC anti-mouse NK-1.1 PK136 FC
PE anti-mouse NK-1.1 PK136 FC
Purified anti-mouse NK-1.1 PK136 FC,CyTOF®,IP,Depletion,Block,IHC-F
PE/Cyanine7 anti-mouse NK-1.1 PK136 FC
PE/Cyanine5 anti-mouse NK-1.1 PK136 FC
Alexa Fluor® 488 anti-mouse NK-1.1 PK136 FC
Alexa Fluor® 647 anti-mouse NK-1.1 PK136 FC
Pacific Blue™ anti-mouse NK-1.1 PK136 FC
Brilliant Violet 711™ anti-mouse NK-1.1 PK136 FC
APC/Cyanine7 anti-mouse NK-1.1 PK136 FC
PerCP anti-mouse NK-1.1 PK136 FC
PerCP/Cyanine5.5 anti-mouse NK-1.1 PK136 FC
Alexa Fluor® 700 anti-mouse NK-1.1 PK136 FC
Brilliant Violet 421™ anti-mouse NK-1.1 PK136 FC,SB
Brilliant Violet 570™ anti-mouse NK-1.1 PK136 FC
Brilliant Violet 650™ anti-mouse NK-1.1 PK136 FC
Brilliant Violet 510™ anti-mouse NK-1.1 PK136 FC
Brilliant Violet 605™ anti-mouse NK-1.1 PK136 FC
Purified anti-mouse NK-1.1 (Maxpar® Ready) PK136 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse NK-1.1 PK136 FC
Brilliant Violet 785™ anti-mouse NK-1.1 PK136 FC
APC/Fire™ 750 anti-mouse NK-1.1 PK136 FC
TotalSeq™-A0118 anti-mouse NK-1.1 PK136 PG
Ultra-LEAF™ Purified anti-mouse NK-1.1 PK136 FC,CyTOF®,IP,Depletion,Block,IHC-F
TotalSeq™-B0118 anti-mouse NK-1.1 PK136 PG
TotalSeq™-C0118 anti-mouse NK-1.1 PK136 PG
PE/Fire™ 810 anti-mouse NK-1.1 PK136 FC
PE/Fire™ 640 anti-mouse NK-1.1 PK136 FC
Spark UV™ 387 anti-mouse NK-1.1 PK136 FC
PE/Fire™ 700 anti-mouse NK-1.1 PK136 FC
Go To Top Version: 2    Revision Date: 12.08.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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