- Clone
- 6-His (See other available formats)
- Regulatory Status
- RUO
- Other Names
- polyhistidine-tag, hexa histidine-tag, 6xHis-tag, His6 tag, His-tag(TM)
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![6-His_DB-HRP_6-His_Epitope_Tag_Antibody_051118 6-His_DB-HRP_6-His_Epitope_Tag_Antibody_051118](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/6-His_DB-HRP_6-His_Epitope_Tag_Antibody_051818_updated.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot analysis of 15 µg cell lysates from HeLa cells transfected with (+) or without (−) His-tagged plasmid. Samples were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 0.05, 0.1 and 0.5 µ/ml Direct-Blot™ HRP, or 0.5 µg/ml purified anti-His-HRP antibody, clone 6-His. Direct-Blot™ HRP anti-GAPDH was used as a loading control (Cat. No. 649203, 1:1000 dilution).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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906109 | 25 µL | 90€ | ||||
906110 | 100 µL | 272€ |
His tags are commonly used as a method of tagging proteins for detection or purification. This antibody is raised again a polyhistidine tag (6-His or hexa His-tag).
Product DetailsProduct Details
- Verified Reactivity
- Epitope tag
- Reported Reactivity
- Species independent
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Monoclonal 6-His antibody was raised against a recombinant protein containing the sequence HHHHHH.
- Purity
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
- Application
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WB - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 5.0 µg per ml (1:1000-1:10000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.
25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution. - Application Notes
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The sequence of the protein flanking the poly His tag does not influence antibody binding. This antibody was subsequently purified via a protein-G column.
- Application References
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- Deinert K, et al. 2001. J. Biol. Chem. 276:6000. (WB)
- Kojima H, et al. 2001. J. Biol. Chem.(22)276:19238. (ICC) PubMed
- Lapinski PE, et al. 2000. J. Biol. Chem. 275:6831. (WB)
- Bartsch D, et al. 1998. Cell. 95:211. (ICC)
- Fuentes EJ, et al. 2003. J Biol Chem. 278:2118. PubMed
- Montegna EA, et al. 2012 PLoS One.7:31156. (WB) PubMed
- Huang L, et al. 2013. Nat Biotechnol. 31:450. (WB) PubMed
- Product Citations
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- RRID
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AB_2734681 (BioLegend Cat. No. 906109)
AB_2734682 (BioLegend Cat. No. 906110)
Antigen Details
- Biology Area
- Cell Biology
- Gene ID
- NA
- UniProt
- View information about 6-His on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All 6-His Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Anti-6-His Epitope Tag Affinity Matrix | 6-His | IP,Purification |
Biotin anti-6-His Epitope Tag | 6-His | WB,ICC,IP |
FITC anti-6-His Epitope Tag | 6-His | ICC |
Purified anti-6-His Epitope Tag | 6-His | WB,ICC,IP |
Direct-Blot™ HRP anti-6-His Epitope Tag | 6-His | WB |
Alexa Fluor® 647 anti-6-His Epitope Tag | 6-His | ICC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Anti-6-His Epitope Tag Affinity Matrix
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Biotin anti-6-His Epitope Tag
Western blot analysis of 0.5 µl Posi-tag (Cat. No. 931301) u... Immunofluorescence of His tagged plasmid transfected A549 ce... -
FITC anti-6-His Epitope Tag
ICC staining of FITC anti-6-His Epitope Tag antibody (clone ... -
Purified anti-6-His Epitope Tag
Western Blot showing specificity of 6-His Monoclonal Antibod... Hela cells transfected with multiple tag plasmid were fixed ... -
Direct-Blot™ HRP anti-6-His Epitope Tag
Western blot analysis of 15 µg cell lysates from HeLa cells ... -
Alexa Fluor® 647 anti-6-His Epitope Tag
HeLa cells were transfected with (B-D) or without (A) His-ta...
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