- Clone
- 1E8 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Keratin, type II cytoskeletal 8, CK-8, keratin-8, cytokeratin-8, K8
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1E8_PURE_Cytokeratin8_Antibody_1_IHC_051716 1E8_PURE_Cytokeratin8_Antibody_1_IHC_051716](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/1E8_PURE_Cytokeratin8_Antibody_1_WB_011918.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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IHC staining of anti-cytokeratin 8 antibody (clone 1E8) on formalin-fixed paraffin-embedded normal human skin. Following antigen retrieval using sodium citrate, the tissue was incubated with the primary antibody at 1 µg/ml for 60 minutes at room temperature. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided. -
Human paraffin-embedded colon tissue slices were prepared with a standard protocol of deparaffinization, rehydration, antigen retrieval, and blocking. Tissue was stained with purified anti-Cytokeratin 8 antibody (clone 1E8, red). Nuclei were counterstained with DAPI (blue). -
Western blot of anti-cytokeratin 8 antibody (clone 1E8). Lane 1: Molecular Weight Marker; Lane 2: HEPG2 lysate; Lane 3: A431 lysate. The blot was incubated with the primary antibody at 1 µg/ml for 60 minutes at room temperature, followed by incubation with a fluorescent labeled goat anti-mouse secondary antibody. -
Total lysates (15 µg protein) from HaCaT (Positive control) and THP-1 (Low expression negative control) were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1 µg/ml) purified anti- Cytokeratin 8 antibody, clone 1E8. Proteins were visualized using chemiluminescence detection by incubation with HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin was used as a loading control (Cat. No. 643807, 1:8000 dilution).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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904804 | 100 µg | 203€ |
Cytokeratin 8 copolymerizes with cytokeratin 18 to form a component of the cytoskeleton in simple epithelia and many epithelial cell derived cancers. It is a member of the Type II keratin family clustered on the long arm of chromosome 12.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Reported Reactivity
- Dog, Non-Human Primate
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This antibody was raised against the 12 carboxy-terminal amino acids of HK-8 preceded by a cysteine, CKLVSESSDVLPK.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
WB - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.1 - 10 μg/ml is suggested. For Western blotting, a concentration range of 0.5 - 5.0 μg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunofluorescence microscopy4.
- Application References
- Product Citations
-
- RRID
-
AB_2616821 (BioLegend Cat. No. 904804)
Antigen Details
- Structure
- Cytokeratin 8 is a 483 amino acid protein with a molecular mass of 54 kD.
- Distribution
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Tissue distribution: colon, small intestine, pancreas, liver, stomach, and salivary glands.
Cellular distribution: extracellular and nucleus. - Function
- Cytokeratin 8 plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Together with KRT19, it helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Intermediate Filaments
- Gene ID
- 3856 View all products for this Gene ID
- UniProt
- View information about Cytokeratin 8 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All Cytokeratin 8 Reagents Request Custom ConjugationDescription | Clone | Applications |
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Purified anti-Cytokeratin 8 | 1E8 | IHC-P,WB |
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