- Clone
- Poly19051 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Loricrin
- Isotype
- Rabbit Polyclonal
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1_Poly19051_PURE_Loricrin_Antibody_1_120319 1_Poly19051_PURE_Loricrin_Antibody_1_120319](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/Poly19051_PURE_Loricrin_Antibody_1_120319.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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IHC staining of purified anti-Loricrin antibody (clone Poly19051) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, AEC, Cat. No. 929401) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 10X objective. Scale bar: 50 µm -
IHC staining of purified anti-Loricrin antibody (clone Poly19051) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, AEC, Cat. No. 929401) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
Western blot of purified anti-Loricrin antibody (clone Poly19051). Lane 1: Molecular weight marker; Lane 2: 20 µg of A431 cell lysate; Lane 3: 20 µg of HepG2 cell lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP donkey anti-rabbit IgG antibody (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system. -
Total lysates (15 µg protein) from HaCaT (human, positive control) and U87-MG (human, negative control) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1 µg/ml) purified anti-Loricrin antibody, clone Poly19051. Proteins were visualized using chemiluminescence detection by incubation with HRP Donkey anti-Rabbit secondary antibody (Cat. No. 406401, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 643807, 1:8000 dilution).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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905103 | 25 µg | 100€ | ||||
905104 | 100 µg | 280€ |
Loricrin is a major protein component of the cornified cell envelope found in terminally differentiated epidermal cells. Mutations in this gene are associated with Vohwinkel's syndrome and progressive symmetric erythrokeratoderma, both inherited skin diseases.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- This antibody was raised against a peptide sequence derived from the C-terminus of the mouse loricrin protein.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
WB - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemisty, a concentration range of 1.0 - 10 μg/mL is suggested. For Western blotting, a usage of 1.0 μg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This product may contain other non-IgG subtypes.
- Application References
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- Suzuki K, et al. 2009. Development 136:367. (IHC-P) PubMed
- Product Citations
-
- RRID
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AB_2734676 (BioLegend Cat. No. 905103)
AB_2616895 (BioLegend Cat. No. 905104)
Antigen Details
- Structure
- Loricrin is a 312 amino acid protein with a molecular mass of 26 kD. Observed MW for human may also be 35 kD.
- Distribution
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Tissue distribution: skin.
Cellular distribution: nucleus and plasma membrane. - Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure
- Antigen References
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1. Wallace L. 2012. J. Cell Sci. 125:1750. (IHC, WB) PubMed
2. Bayo P, et al. 2008. Endocrinology 149:1377. (IHC) PubMed
3. Hu Y, et al. 2001. Nature 410:710. PubMed
4. Croyle MJ, et al. 2011. Development 138:1675. (IF) PubMed
5. Sengupta A, et al. 2010. PLoS One 5(8):e12249. (IHC) PubMed
6. Liakath-Ali K, et al. 2014. Nat. Commun. 5:3540. (IF) PubMed - Gene ID
- 4014 View all products for this Gene ID
- UniProt
- View information about Loricrin on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All Loricrin Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Loricrin | Poly19051 | IHC-P,WB |
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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