- Clone
- BL26881 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![BL26881_PURE_S-tag_Antibody_1_WB_092216 BL26881_PURE_S-tag_Antibody_1_WB_092216](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/BL26881_PURE_S-tag_Antibody_1_WB_092216.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Recombinant Posi-Tag Epitope Tag Protein was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-S-Tag (clone BL26881) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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688102 | 25 µg | 72€ |
S-tag is the name of an oligopeptide derived from pancreatic RNase A. The N-terminus of the original RNase A, also called S-peptide, consists of 20 amino acid residues, of which only the first 15 are required for ribonuclease activity. This 15 amino acids long peptide is called S15 or S-tag. The S-Tag sequence is a fusion peptide tag for recombinant proteins that allows detection by a rapid, sensitive homogeneous assay or by colorimetric detection in Western blots. Proteins can also be rapidly purified from crude extracts using affinity separation method.
Product DetailsProduct Details
- Verified Reactivity
- Epitope tag
- Reported Reactivity
- Species independent
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic Peptide
- Formulation
- Liquid in phosphate-buffered solution containing 50% glycerol, 0.25% BSA and 0.02% sodium azide.
- Preparation
- The antibody was affinity purified from mouse ascites by affinity chromatography using specific immunogen.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C.
- Application
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WB - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
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- RRID
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AB_2629593 (BioLegend Cat. No. 688102)
Antigen Details
- Structure
- The amino acid sequence of the S-tag is: Lys-Glu-Thr-Ala-Ala-Ala-Lys-Phe-Glu-Arg-Gln-His-Met-Asp-Ser.
- Distribution
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Derived from pancreatic ribonuclease A (RNase A).
- Function
- S-tag can be attached to the N- or C-terminus of protein to become S-tag fused protein.
- Biology Area
- Cell Biology
- Antigen References
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1. Raines RT, et al. 2000. Methods Enzymol. 326:362.
- Gene ID
- NA
- UniProt
- View information about S-Tag on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All S-Tag Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-S-Tag | BL26881 | WB |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-S-Tag
Recombinant Posi-Tag Epitope Tag Protein was resolved by ele...
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