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PE/Cyanine7 Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
SAv-PE/Cyanine7
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Product Citations
publications
PECyanine7_Streptavidin_Antibody_042123
Human peripheral blood lymphocytes were stained with PE/Cyanine7 streptavidin alone (open histogram) or Biotin anti-human CD3 (clone UCHT1) followed by (PE/Cyanine7) Streptavidin (filled histogram).
  • PECyanine7_Streptavidin_Antibody_042123
    Human peripheral blood lymphocytes were stained with PE/Cyanine7 streptavidin alone (open histogram) or Biotin anti-human CD3 (clone UCHT1) followed by (PE/Cyanine7) Streptavidin (filled histogram).
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
405206 100 µg 206 CHF
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Description

Streptavidin binds to biotin with high affinity. Streptavidin - Phycoerythrin/Cyanine7 (PE/Cyanine7) is useful for detecting biotinylated antibodies. The excitation of PE/Cyanine7 by 488 nm laser light induces a light emission maximum of 775 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin-PE/Cyanine7 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

ICFC - Verified
Recommended Usage

Each lot of this Streptavidin-PE/Cyanine7 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular molar mass of streptavidin independent of any additional molecular mass that might be added by the PE/Cyanine7 conjugation.  For immunofluorescent staining, the recommended use of this reagent is =0.125 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Streptavidin - Phycoerythrin/Cyanine7 (PE/Cyanine7) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-PE/Cyanine7 is 360 kD and Streptavidin alone is 52 kD.

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
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Antigen Details

Gene ID
NA
Specificity (DOES NOT SHOW ON TDS):
Biotin
Specificity Alt (DOES NOT SHOW ON TDS):
Biotin
App Abbreviation (DOES NOT SHOW ON TDS):
FC,ICFC
UniProt
View information about Biotin on UniProt.org
Go To Top Version: 5    Revision Date: 04.21.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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