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Purified anti-DYKDDDDK Tag Antibody

Pricing & Availability
Clone
L5 (See other available formats)
Regulatory Status
RUO
Other Names
FLAG tag
Isotype
Rat IgG2a, λ
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Product Citations
publications
L5_WB_100608
Cell extracts from untransfected 293T cells (lane 1) or 293T cells transfected with a plasmid encoding DYKDDDDK-tagged protein (lane 2), using anti-DYKDDDDK, clone L5.
  • L5_WB_100608
    Cell extracts from untransfected 293T cells (lane 1) or 293T cells transfected with a plasmid encoding DYKDDDDK-tagged protein (lane 2), using anti-DYKDDDDK, clone L5.
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637319 25 µg 89 CHF
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637301 200 µg 253 CHF
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637302 500 µg 382 CHF
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637303 1 mg 475 CHF
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637304 5 mg 780 CHF
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Description

The DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins.This unique amino acid sequence allows for specific antibody detection in western blotting, immunoprecipitation, and immunostaining techniques.Due to the short sequence, this modification is not likely to affect the structure or function of the modified proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Epitope tag
Reported Reactivity
Species independent
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
DYKDDDDK-tagged mouse Langerin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP, ICC, ELISA, FC, Purification - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The L5 clone has been demonstrated to have 2-8 fold better sensitivity in WB than another commonly used antibody clone, M2.

Application References

(PubMed link indicates BioLegend citation)
  1. Park SH, et al. 2008. J Immunol Methods. 331:27.
  2. Moon SH, et al. 2010. J. Biol Chem. 285:12935. PubMed
  3. Sasaki M, et al. 2011. J. Biol Chem. 286:39370. PubMed
  4. Sonder SU, et al. 2012. J Immunol. 188:5906. PubMed
  5. Jiang Y, et al. 2013. Int Immunol. 25:235. PubMed
  6. Zuo X, et al. 2014. PLoS One. 9:84748. PubMed
  7. Toyo-Oak K, et al. 2014. J Neurosci. 34:12168. PubMed
Product Citations
  1. DaRosa PA et al. 2018. Molecular cell. 72(4):753-765 . PubMed
  2. Mamedov T, et al. 2019. PLoS One. 14:e0213438. PubMed
  3. Trothen SM, et al. 2022. FEBS Lett. 596:232. PubMed
  4. Sun X, et al. 2022. Appl Environ Microbiol. 88:e0230321. PubMed
  5. Basavarajappa SC, et al. 2022. iScience. 25:104716. PubMed
  6. Wang W, et al. 2022. Cell Rep. 41:111582. PubMed
  7. Meleppattu S, et al. 2022. Cell. 185:4986. PubMed
  8. Setiaputra D, et al. 2022. Mol Cell. 82:1359. PubMed
  9. Kluss JH, et al. 2022. Neurobiol Dis. 170:105769. PubMed
  10. Chaouat AE, et al. 2022. iScience. 25:104935. PubMed
  11. Yang A, et al. 2023. J Immunother Cancer. 11:. PubMed
  12. Willcox CR, et al. 2020. Immunity. 51(5):813-825.e4.. PubMed
  13. Bala Tannan N, et al. 2018. PLoS Genet. 14:e1007153. PubMed
  14. Asano Y, et al. 2021. Nat Commun. 12:4372. PubMed
  15. Zhu C, et al. 2020. Cell Rep. 31:107745. PubMed
  16. Wroblewska A et al. 2018. Cell. 175(4):1141-1155 . PubMed
  17. Chaouat AE, et al. 2021. PLoS Pathog. 17:e1010175. PubMed
  18. Das A, et al. 2016. J Biol Chem. 291: 6096 - 6110. PubMed
  19. Mamedov T, et al. 2017. PLoS One.. 10.1371/journal.pone.0183589. PubMed
  20. Wang X, et al. 2018. Pathog Dis. 76:. PubMed
  21. Sundaram A et al. 2017. eLife. 6 pii: e27187. PubMed
  22. Yang M, et al. 2020. Appl Environ Microbiol. 86:00:00. PubMed
  23. Wen XY, et al. 2018. JCI Insight. 3:. PubMed
  24. Sánchez-Bellver L, et al. 2022. Int J Mol Sci. 23:. PubMed
  25. He H, et al. 2021. J Immunother Cancer. 9:. PubMed
  26. Sims S, et al. 2021. Adv Virol. 2021:5569844. PubMed
  27. Nabar NR, et al. 2022. Autophagy. 18:204. PubMed
  28. Bonet-Ponce L, et al. 2020. Sci Adv. 6:00. PubMed
  29. Ren S, et al. 2016. PLoS One. 11: 0152721. PubMed
  30. Jiang Y, et al. 2013. Int Immunol. 25:235. PubMed
  31. Sønder S, et al. 2012. J Immunol. 188:5906. PubMed
  32. Toyo-oka K, et al. 2014. J Neurosci. 34:12168. PubMed
  33. Shi Y, et al. 2016. Cell Death Differ. 10.1038/cdd.2016.110. PubMed
  34. Golec E, et al. 2019. FASEB J. 33:12425. PubMed
  35. Velay F, et al. 2022. Plant Methods. 18:69. PubMed
  36. Adam S, et al. 2021. Nat Cancer. 2:1357. PubMed
  37. Karunakaran MM, et al. 2020. Immunity. 52:487. PubMed
  38. Yin J, et al. 2015. Brain. 138: 2553-2570. PubMed
  39. Park GY, et al. 2020. Immune Netw. e43:20. PubMed
  40. Moon S, et al. 2010. J Biol Chem. 285:12935. PubMed
  41. Yuan Y, et al. 2020. Nat Commun. 11:1472. PubMed
  42. Zang RX, et al. 2022. ACS Omega. 7:34378. PubMed
  43. Kim J, et al. 2021. J Cell Biol. 220:. PubMed
  44. Zhang S, et al. 2020. Nat Commun. 11:1312. PubMed
  45. Carlevaro G, et al. 2019. Methods Mol Biol. 1955:135. PubMed
  46. Okamoto Y, et al. 2021. J Cell Sci. 134:. PubMed
  47. Fujii T, et al. 2021. Bone Res. 9:4. PubMed
  48. Zuo X, et al. 2014. PLoS One. 9:84748. PubMed
  49. Stroukov W, et al. 2019. Front Genet. 10:163. PubMed
  50. Cross SH, et al. 2020. PLoS Genet. 16:e1008583. PubMed
  51. Takahashi K, et al. 2020. Cell Reports. 31(9):107715. PubMed
  52. Liu X, et al. 2021. Hum Mol Genet. 30:30. PubMed
  53. Hu J, et al. 2021. Cell Death Dis. 12:391. PubMed
  54. Sandhu R, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  55. Xu C, et al. 2019. J Immunol. 203:2141. PubMed
  56. Wu G, et al. 2021. Nat Cancer. 2:1170. PubMed
  57. Liu X, et al. 2019. Nat Commun. 10:3020. PubMed
  58. Mamedov T, et al. 2019. Sci Rep. 9:9868. PubMed
  59. Driskill JH, et al. 2021. Genes Dev. 35:495. PubMed
  60. Figueroa F, et al. 2021. Front Cell Dev Biol. 9:739445. PubMed
  61. Sharma VP, et al. 2021. Nat Commun. 12:7300. PubMed
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RRID
AB_2749907 (BioLegend Cat. No. 637319)
AB_1134266 (BioLegend Cat. No. 637301)
AB_1134268 (BioLegend Cat. No. 637302)
AB_1134265 (BioLegend Cat. No. 637303)
AB_1134267 (BioLegend Cat. No. 637304)

Antigen Details

Biology Area
Cell Biology
Antigen References

1. Einhauer A. 2001. J. Biochem. Biophys. Methods. 49:455.
2. Knappik A and Pluckthun A. 1994. Biotechniques. 17:754.

Gene ID
NA
Specificity (DOES NOT SHOW ON TDS):
DYKDDDDK
Specificity Alt (DOES NOT SHOW ON TDS):
DYKDDDDK
App Abbreviation (DOES NOT SHOW ON TDS):
WB,IP,ICC,ELISA,FC,Purification
UniProt
View information about DYKDDDDK on UniProt.org

Related FAQs

I am unable to detect FLAG tag with L5 antibody clone by IP and/or western blotting.
Some points to consider here include
  • Sometimes changing tag location (form N-terminus to C-terminus or vice versa) or use tandem FLAG tag (3x) can also increase the chances of tag detection
  • Tagged protein is either not expressed or lowly expressed
  • Always include a positive control to make sure the process of working fine
Does anti tag antibody recognize the tag sequence at N terminal or C terminal or within the fusion protein?
Our antibody can recognize the fusion protein with tag either at N-terminus or C-terminus or within the protein if the tag is exposed.
Go To Top Version: 4    Revision Date: 09.21.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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