- Clone
- W17215A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- 3-Phosphoinositide-Dependent Protein Kinase 1, PDK1
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W17215A_Purified_PDPK1_Antibody_051719.png W17215A_Purified_PDPK1_Antibody_051719.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W17215A_Purified_PDPK1_Antibody_051719.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-PDPK1 Antibody, clone W17215A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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617601 | 25 µg | 101 CHF | ||||
617602 | 100 µg | 253 CHF |
3-Phosphoinositide-Dependent Protein Kinase 1 (PDPK1) is serine/threonine kinase that plays a central role in diverse cellular pathways. It functions by phosphorylating and activating members of the AGC family of serine kinases, including Akt1, Protein Kinases A and C, and p70 ribosomal protein S6 kinase. Loss or reduction of PDPK1 in the thymus results in defective T-cell differentiation and proliferative expansion.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial recombinant human PDPK1 protein corresponding to amino acid residues 411-556
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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WB - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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This clone is predicted to recognize rat PDPK1 due to high sequence conservation between the immunizing protein and the rat homolog.
This clone is predicted to recognize PDPK1 isoforms 1-4 due to the presence of the immunizing sequence in each isoform.
This clone failed ICC and IP testing. - RRID
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AB_2810673 (BioLegend Cat. No. 617601)
AB_2810674 (BioLegend Cat. No. 617602)
Antigen Details
- Structure
- PDPK1 is a 556 amino acid protein with a predicted molecular weight of 63 kDa. Four PDPK1 isoforms exist ranging in size from 48-63 kD.
- Distribution
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Peripheral plasma membrane protein/ubiquitously expressed
- Function
- Regulator of insulin, PKC, Akt1, and SMAD signal tranduction
- Biology Area
- Cell Biology
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
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- Le Good JA, et al. 1998. Science. 281:2042.
- Dong LQ, et al. 2000. Proc. Natl. Acad. Sci. 97:5089.
- Hinton HJ, et al. 2004. Nat. Immunol. 5:539.
- Gene ID
- 5170 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- PDPK1
- Specificity Alt (DOES NOT SHOW ON TDS):
- PDPK1
- App Abbreviation (DOES NOT SHOW ON TDS):
- WB
- UniProt
- View information about PDPK1 on UniProt.org
Related FAQs
Other Formats
View All PDPK1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-PDPK1 | W17215A | WB |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-PDPK1
Whole cell extracts (15 µg protein) from the indicated cell ...
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