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Purified anti-SOX17 Antibody

Pricing & Availability
Clone
O92B6 (See other available formats)
Regulatory Status
RUO
Other Names
Transcription factor SOX-17, SOX-17, SOX 17, SRY-Box17, SRY-Related HMG-Box Transcription Factor SOX17.
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
O92B6_PURE_SOX17_1_WB_121019
Whole cell extracts (15 µg protein) from HeLa (negative control) and OVCAR-3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-Sox17 antibody, clone O92B6, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 643807) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • O92B6_PURE_SOX17_1_WB_121019
    Whole cell extracts (15 µg protein) from HeLa (negative control) and OVCAR-3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-Sox17 antibody, clone O92B6, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 643807) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • O92B6_PURE_SOX17_2_IF_101917.png
    OVCAR3 cells were fixed with 4% paraformaldehyde (PFA) for fifteen minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1:200 diluted (2.5µg/mL), anti-SOX17 (clone O92B6), overnight at 4°C followed by 1:200 diluted (2.5µg/mL) Alexa Fluor® 594 (red) conjugated goat anti-mouse IgG for anti-SOX17antibody, for one hour at room temperature. Actin filaments were labeled with Flash Phalloidin™ Green 488 (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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Description

SOX17 is an important member of the SOX family, which is involved in various developmental processes including germ layer formation, cell-type specification, and organogenesis. SOX17 activates endodermal target genes, and is required for the formation of endoderm and blood vessel endothelium. SOX17 also plays critical roles in the maintenance of fetal and neonatal hematopoietic stem cells.

SOX17 is also proved to be a tumor suppressor through suppression of Wnt signaling in gastrointestinal carcinomas. SOX17 expression is down-regulated in colon cancer cells by methylating promoter, and the reduced expression of SOX17 in colon cancer cells mitigates the efficiency of the colony formation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein expressed in E. Coli corresponding to aa 177-414 of human SOX17.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified 
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg/mL (1:1000 - 1:250 dilution). For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL (1:500 - 1:100 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Fu Q, et al. 2021. Stem Cell Research. 54:102428. PubMed
  2. Feng H, et al. 2021. Stem Cell Research. 53:102346. PubMed
  3. Ruan J, et al. 2019. Mol Ther Nucleic Acids. 16:73. PubMed

Antigen Details

Structure
414 amino acids, and predicted Mw is 44kDa.
Distribution

Nucleus

Function
Acts as transcription regulator that binds target promoter DNA and bends the DNA.
Interaction
Interacts with CTNNB1, LEF1 and TCF4.
Biology Area
Cell Biology, Signal Transduction, Transcription Factors
Antigen References

1. Wegner M. 1999. Nucleic Acids Res. 27:1409.
2. Du YC, et al. 2009. Gastroenterology 137:1346.
3. Zorn AM, et al. 1999. Mol. Cell. 4:487.
4. Sinner D, et al. 2004. Development 131:3069.
5. Hudson C, et al. 1997. Cell. 91:397.
6. Matsui T, et al. 2006. J. Cell. Sci. 119:3513.
7. Kim I, et al. 2007. Cell. 130:470.
8. Zhang W, et al. 2008. Cancer Res. 68:2764.

Gene ID
64321 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
SOX17
Specificity Alt (DOES NOT SHOW ON TDS):
SOX17
App Abbreviation (DOES NOT SHOW ON TDS):
WB,ICC
UniProt
View information about SOX17 on UniProt.org
Go To Top Version: 3    Revision Date: 12.10.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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