- Clone
- J099B12 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Histidine Tag
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![J099B12_A488_HisTag_Antibody_100617 J099B12_A488_HisTag_Antibody_100617](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/J099B12_A488_HisTag_Antibody_100617.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
Hela cells transfected with multi-tag plasmid were fixed with cold methanol for 15 minutes at -20°C and then blocked with 5% FBS for 60 minutes. Cells were then stained with either (A) 1:500 (1 µg/ml) Alexa Fluor® 488 mouse IgG1, κ isotype control antibody (Cat no. 400133), or (B, C) 1:500 (1 µg/ml), (D) 1:1000 (0.5 µg/ml) or (E) 1:2500 (0.2 µg/ml) Alexa Fluor® 488 anti-His antibody (green), and incubated overnight at 4°C. Nuclei were counterstained with DAPI (blue, Cat no. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (S): (A) 1/15, (B, D, E) 1/50, (C) 1/150.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
652509 | 25 µg | 159 CHF |
This antibody was raised against linear 8XHis tag. It reacts with His tag protein, either C, N, or internal His6 and up repeats. It can be used for Western blotting to detect linear His repeat (His6 or more).
Product DetailsProduct Details
- Verified Reactivity
- Epitope tag
- Reported Reactivity
- Species independent
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- KLH-conjugated His-tag peptide
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.2 - 0.5 μg/ml (Dilution 1:1000-1:2500) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
-
Application References
(PubMed link indicates BioLegend citation) -
- Burritt JB, et al. 1995. J. Biol. Chem. 270:16974.
- Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.
- RRID
-
AB_2716151 (BioLegend Cat. No. 652509)
Antigen Details
- Function
- Recombinant poly-histidine tag detection
- Biology Area
- Cell Biology
- Gene ID
- NA
- Specificity (DOES NOT SHOW ON TDS):
- His Tag
- Specificity Alt (DOES NOT SHOW ON TDS):
- His Tag
- App Abbreviation (DOES NOT SHOW ON TDS):
- ICC
- UniProt
- View information about His Tag on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All His Tag Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-His Tag | J099B12 | WB,ICC |
HRP anti-His Tag | J099B12 | WB,ELISA |
Ultra-LEAF™ Purified anti-His Tag | J099B12 | WB |
Alexa Fluor® 488 anti-His Tag | J099B12 | ICC |
Alexa Fluor® 594 anti-His Tag | J099B12 | ICC |
Alexa Fluor® 647 anti-His Tag | J099B12 | ICC |
Customers Also Purchased
![](https://d1spbj2x7qk4bg.cloudfront.net/Files//Images/media_assets/products/product_images/J099B12_PURE_His-tag_Antibody_111717_UPDATED.png)
![](https://d1spbj2x7qk4bg.cloudfront.net/Files//Images/media_assets/products/product_images/30-F11_PE_090507.jpg)
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-His Tag
Western blot analysis of cell lysates from HeLa cells transf... Immunofluorescence of HeLa cells transfected without (A) or ... Insect cell supernatant expressing His-tagged mouse CCL22 wa... -
HRP anti-His Tag
Whole cell extracts (15 µg total protein) from HEK293 cells ... ELISA assay was performed by coating a 96-well plate with 10... -
Ultra-LEAF™ Purified anti-His Tag
0.1µl recombinant Posi-Tag Epitope Tag Protein was resolved ... -
Alexa Fluor® 488 anti-His Tag
Hela cells transfected with multi-tag plasmid were fixed wi... -
Alexa Fluor® 594 anti-His Tag
Hela cells transfected with multi-tag plasmid were fixed wit... -
Alexa Fluor® 647 anti-His Tag
Hela cells transfected with multi-tag plasmid were fixed wit...
Follow Us