- Clone
- AK4 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- VI P-44
- Other Names
- GMP-140, PADGEM
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![AK4_APCCyanine7_CD62P_Antibody_041219.png AK4_APCCyanine7_CD62P_Antibody_041219.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/AK4_APCCyanine7_CD62P_Antibody_041219.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Thrombin-activated human peripheral blood platelets were stained with CD62P (P-Selectin) (clone AK4) APC/Cyanine7 (filled histogram) or mouse IgG1, κ APC/Cyanine7 isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
304943 | 25 tests | 141 CHF | ||||
304944 | 100 tests | 352 CHF |
CD62P is a 140 kD type I transmembrane glycoprotein also known as P-selectin, platelet activation-dependent granule membrane protein (PADGEM), and GMP-140. It is expressed on activated platelets, megakaryocytes, and endothelial cells. CD62P is primarily stored in secretory α-granules in platelets and Weibel-Palade bodies in endothelial cells, and is rapidly relocated to the plasma membrane upon activation. The ligands for CD62P are CD162 and CD24. A primary function of CD62P is cell adhesion during neutrophil rolling, and platelet-neutrophil and platelet-monocyte interactions.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections4 and in vitro blocking of adhesion of platelets1. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 304947 & 304948).
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Application References
(PubMed link indicates BioLegend citation) -
- Skinner M, et al. 1991. J. Biol. Chem. 266:5371. (Block)
- Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
- Yen YT, et al. 2006. J. Virol. 80:2684.
- Sato Y, et al. 2005. Blood 106:428. (IHC)
- RRID
-
AB_2810453 (BioLegend Cat. No. 304943)
AB_2810454 (BioLegend Cat. No. 304944)
Antigen Details
- Structure
- Selectin, type I glycoprotein, 140 kD
- Distribution
-
Activated platelets, megakaryocytes, endothelial cells
- Function
- Adhesion, neutrophil rolling, platelet-neutrophil and platelet-monocyte interactions
- Ligand/Receptor
- CD162 (PSGL-1), CD24 and sialylated Lewis X
- Cell Type
- Endothelial cells, Megakaryocytes, Neutrophils, Platelets
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Neuroscience, Synaptic Biology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. McEver R, et al. 1995. J. Biol. Chem. 270:11025.
2. Varki A. 1994. P. Natl. Acad. Sci. USA 91:7390. - Gene ID
- 6403 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- CD62P
- Specificity Alt (DOES NOT SHOW ON TDS):
- CD62P
- App Abbreviation (DOES NOT SHOW ON TDS):
- FC
- UniProt
- View information about CD62P on UniProt.org
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human CD62P (P-Selectin)
Thrombin-activated human peripheral blood platelets stained ... -
FITC anti-human CD62P (P-Selectin)
Thrombin-activated human peripheral blood platelets stained ... -
PE anti-human CD62P (P-Selectin)
Thrombin-activated human platelets stained with AK4 PE -
PE/Cyanine5 anti-human CD62P (P-Selectin)
Thrombin-activated human peripheral blood platelets stained ... -
Purified anti-human CD62P (P-Selectin)
Thrombin-activated human peripheral blood platelets stained ... -
Biotin anti-human CD62P (P-Selectin)
Thrombin-activated human platelets stained with biotinylated... -
Alexa Fluor® 488 anti-human CD62P (P-Selectin)
Thrombin-activated human peripheral blood platelets stained ... -
Alexa Fluor® 647 anti-human CD62P (P-Selectin)
Thrombin-activated human platelets stained with AK4 Alexa Fl... -
Brilliant Violet 605™ anti-human CD62P (P-Selectin)
Thrombin-activated human peripheral blood platelets were sta... -
Brilliant Violet 421™ anti-human CD62P (P-Selectin)
Thrombin-activated human peripheral blood platelets were sta... -
PE/Cyanine7 anti-human CD62P (P-Selectin)
Thrombin-activated human peripheral blood platelets were sta... -
PerCP/Cyanine5.5 anti-human CD62P (P-Selectin)
Thrombin-activated human peripheral blood platelets were sta... -
Alexa Fluor® 700 anti-human CD62P (P-Selectin)
Thrombin-activated human peripheral blood platelets were sta... -
PE/Dazzle™ 594 anti-human CD62P (P-Selectin)
Thrombin-activated human peripheral blood platelets were sta... -
APC/Fire™ 750 anti-human CD62P (P-Selectin)
Thrombin-activated human peripheral blood platelets were sta... -
TotalSeq™-A0218 anti-human CD62P (P-Selectin)
-
Brilliant Violet 650™ anti-human CD62P (P-Selectin)
Thrombin-activated human peripheral blood platelets were sta... -
Brilliant Violet 510™ anti-human CD62P (P-Selectin)
Thrombin-activated human peripheral blood platelets were sta... -
Brilliant Violet 711™ anti-human CD62P (P-Selectin)
Thrombin-activated human peripheral blood platelets were sta... -
APC/Cyanine7 anti-human CD62P (P-Selectin)
Thrombin-activated human peripheral blood platelets were sta... -
Brilliant Violet 785™ anti-human CD62P (P-Selectin)
Thrombin-activated human peripheral blood platelets were sta... -
TotalSeq™-C0218 anti-human CD62P (P-Selectin)
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Ultra-LEAF™ Purified anti-human CD62P (P-Selectin)
Thrombin-activated human peripheral blood platelets stained ... -
TotalSeq™-B0218 anti-human CD62P (P-Selectin) Antibody
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TotalSeq™-D0218 anti-human CD62P (P-Selectin)
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PE anti-human CD62P
Typical results from thrombin-activated human peripheral blo... -
FITC anti-human CD62P
Typical results from thrombin-activated human peripheral blo... -
GMP FITC anti-human CD62P (P-Selectin)
Typical results from thrombin-activated human peripheral blo... -
GMP PE anti-human CD62P (P-Selectin)
Typical results from thrombin-activated human peripheral blo...
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