- Clone
- BB7.2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- MHC-class I
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![BB7point2_BV510_HLA-A2_Antibody_1_012816 BB7point2_BV510_HLA-A2_Antibody_1_012816](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/BB7point2_BV510_HLA-A2_Antibody_1_012816.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Peripheral blood lymphocytes from HLA-A2 positive (top) and HLA-A2 negative (bottom) donors were stained with anti-human HLA-A2 (clone BB7.2) Brilliant Violet 510™ (filled histograms) or mouse IgG2b, κ Brilliant Violet 510™ isotype control (open histograms). -
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
343319 | 25 tests | 193 CHF | ||||
343320 | 100 tests | 413 CHF |
HLA-A2 is most common in Northern Asia and North America populations. MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in presentation of antigens to CD8+ T cells, playing an important role in cell-mediated immune responses and tumor surveillance.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Papain solubilized HLA-A2
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
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Violet Laser (405 nm)
- Application Notes
-
The BB7.2 antibody recognizes human leukocyte antigen (HLA) A2 which is a subset of MHC-class I molecules encoded by A*02 alleles.
Additional reported applications (for the relevant formats) include: immunoprecipitation3. -
Application References
(PubMed link indicates BioLegend citation) -
- Brodsky FM, et al. 1979. Immunol. Rev. 47:3.
- Parham P and Brodsky FM. et al. 1981. Hum. Immunol. 3:277.
- Lubben NB, et al. 2007. Mol Biol Cell. 18:3351. (IP)
- Product Citations
-
- RRID
-
AB_2566766 (BioLegend Cat. No. 343319)
AB_2566767 (BioLegend Cat. No. 343320)
Antigen Details
- Structure
- Ig superfamily
- Distribution
-
A*02 individuals leukocytes
- Function
- Antigen presentation
- Ligand/Receptor
- CD8, CD3/TCR
- Cell Type
- Leukocytes
- Biology Area
- Immunology
- Molecular Family
- MHC Antigens
- Gene ID
- 3105 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- HLA-A2
- Specificity Alt (DOES NOT SHOW ON TDS):
- HLA-A2
- App Abbreviation (DOES NOT SHOW ON TDS):
- FC
- UniProt
- View information about HLA-A2 on UniProt.org
Other Formats
View All HLA-A2 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Alexa Fluor® 700 anti-human HLA-A2 | BB7.2 | FC |
Purified anti-human HLA-A2 | BB7.2 | FC,IP |
FITC anti-human HLA-A2 | BB7.2 | FC |
PE anti-human HLA-A2 | BB7.2 | FC |
APC/Cyanine7 anti-human HLA-A2 | BB7.2 | FC |
APC anti-human HLA-A2 | BB7.2 | FC |
Pacific Blue™ anti-human HLA-A2 | BB7.2 | FC |
PE/Cyanine7 anti-human HLA-A2 | BB7.2 | FC |
PerCP/Cyanine5.5 anti-human HLA-A2 | BB7.2 | FC |
Brilliant Violet 510™ anti-human HLA-A2 | BB7.2 | FC |
Biotin anti-human HLA-A2 | BB7.2 | FC |
Brilliant Violet 650™ anti-human HLA-A2 | BB7.2 | FC |
Brilliant Violet 785™ anti-human HLA-A2 | BB7.2 | FC |
Brilliant Violet 421™ anti-human HLA-A2 | BB7.2 | FC |
APC/Fire™ 750 anti-human HLA-A2 | BB7.2 | FC |
PE/Dazzle™ 594 anti-human HLA-A2 Antibody | BB7.2 | FC |
TotalSeq™-B0899 anti-human HLA-A2 | BB7.2 | PG |
TotalSeq™-C0899 anti-human HLA-A2 | BB7.2 | PG |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Alexa Fluor® 700 anti-human HLA-A2
Peripheral blood lymphocytes from HLA-A2 positive (top) and ... -
Purified anti-human HLA-A2
HLA-A2+ (top) and HLA-A2- (bottom) donors' peripheral blood ... -
FITC anti-human HLA-A2
HLA-A2+ (top) and HLA-A2- (bottom) donors’ peripheral ... -
PE anti-human HLA-A2
HLA-A2 positive (top) and HLA-A2 negative (bottom) donors pe... -
APC/Cyanine7 anti-human HLA-A2
Peripheral blood lymphocytes from HLA-A2 positive (top) and ... -
APC anti-human HLA-A2
Peripheral blood lymphocytes from HLA-A2 positive (top) and ... -
Pacific Blue™ anti-human HLA-A2
Peripheral blood lymphocytes from HLA-A2 positive (top) and ... -
PE/Cyanine7 anti-human HLA-A2
Peripheral blood lymphocytes from HLA-A2 positive (top) and ... -
PerCP/Cyanine5.5 anti-human HLA-A2
Peripheral blood lymphocytes from HLA-A2 positive (top) and ... -
Brilliant Violet 510™ anti-human HLA-A2
Peripheral blood lymphocytes from HLA-A2 positive (top) and ... -
Biotin anti-human HLA-A2
Peripheral blood lymphocytes from HLA-A2 positive (top) and ... -
Brilliant Violet 650™ anti-human HLA-A2
Peripheral blood lymphocytes from HLA-A2 positive (top) and ... -
Brilliant Violet 785™ anti-human HLA-A2
Peripheral blood lymphocytes from HLA-A2 positive (top) and ... -
Brilliant Violet 421™ anti-human HLA-A2
Peripheral blood lymphocytes from HLA-A2 positive (top) and ... -
APC/Fire™ 750 anti-human HLA-A2
Peripheral blood lymphocytes from HLA-A2 positive (left) and... -
PE/Dazzle™ 594 anti-human HLA-A2 Antibody
HLA-A2 positive human peripheral blood lymphocytes were stai... HLA-A2 negative human peripheral blood lymphocytes were stai... -
TotalSeq™-B0899 anti-human HLA-A2
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TotalSeq™-C0899 anti-human HLA-A2
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