- Clone
- GHI/61 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- VI M38
- Other Names
- GHI/61, M130, RM3/1, p155, Hemoglobin/Haptoglobin Complex Receptor, macrophage-associated antigen, ED2(rat)
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![GHIslash61_BV785_CD163_Antibody_032918 GHIslash61_BV785_CD163_Antibody_032918](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/GHIslash61_BV785_CD163_Antibody_032918.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood monocytes were stained with CD163 (clone GHI/63) Brilliant Violet 785™ (filled histogram) or mouse IgG1, κ Brilliant Violet 785™ isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
333631 | 25 tests | 187 CHF | ||||
333632 | 100 tests | 418 CHF |
CD163 is a member of the group B scavenger receptor cysteine-rich superfamily, also known as GHI/61, M130, RM3/1, p155, hemoglobin-haptoglobin complex receptor, or macrophage-associated antigen. It is a 134 kD (non-reduced)/155 kD (reduced) glycoprotein primarily expressed on macrophages, Kupffer cells, monocytes, a subset of dendritic cells, and a subset of hematopoietic stem/progenitor cells. CD163 binds to haptoglobin-hemoglobin complex and TWEAK, and plays a role in clearing hemoglobin and regulating cytokine production by macrophages. Membrane CD163 can be cleaved by metalloproteinases (MMP), resulting in a soluble form. Elevated serum level of sCD163 has been implicated in many kinds of inflammatory diseases.
Product DetailsProduct Details
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
Clone GHI/61 binds to domain 7 of CD163. Additional reported applications (for the relevant formats) include: immunocytochemical staining, immunoprecipitation, western blot1, and spatial biology (IBEX)6,7.
-
Application References
(PubMed link indicates BioLegend citation) -
- Pulford K, et al. 1992. Immunology 75:588. (ICC, IP, WB)
- Law SK, et al. 1993. Eur. J. Immunol. 23:2320.
- Madsen M, et al. 2004. J. Biol. Chem. 279:51561.
- Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
- Buttari B, et al. 2011. Atherosclerosis. 215:316. PubMed
- Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
-
- RRID
-
AB_2728287 (BioLegend Cat. No. 333631)
AB_2728288 (BioLegend Cat. No. 333632)
Antigen Details
- Structure
- 134 kD (non-reduced)/155 kD (reduced) glycoprotein, scavenger receptor superfamily
- Distribution
-
Monocytes, macrophages, Kuffer cells, subset of dendritic cells, subset of hematopoietic stem/progenitor cells
- Function
- Clearance of haptoglobin-hemoglobin complex, regulation of cytokine production by macrophages
- Ligand/Receptor
- Haptoglobin-hemoglobin complex, TWEAK
- Cell Type
- Dendritic cells, Hematopoietic stem and progenitors, Macrophages, Monocytes
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
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1. Roth J, et al. 1994 Transolantation. 57:127
2. Van den Heuvel MM, et al.1999 J. Leukoc. Biol. 66:858
3. Sulahian TH, et al. 2000 Cytokines 12:1312
4. Fabriek BO, et al. 2007 J. Neuroimmunol. 187:179 - Gene ID
- 9332 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- CD163
- Specificity Alt (DOES NOT SHOW ON TDS):
- CD163
- App Abbreviation (DOES NOT SHOW ON TDS):
- FC
- UniProt
- View information about CD163 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All CD163 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PerCP anti-human CD163
Human peripheral blood monocytes were stained with CD163 (cl... -
Purified anti-human CD163
Human peripheral blood monocytes stained with purified GHI/6... -
Biotin anti-human CD163
Human peripheral blood monocytes stained with biotinylated G... -
PE anti-human CD163
Human peripheral blood monocytes stained with GHI/61 PE -
PerCP/Cyanine5.5 anti-human CD163
Human peripheral blood lymphocytes, monocytes, granulocytes ... -
APC anti-human CD163
Human peripheral blood monocytes stained with GHI/61 APC -
Brilliant Violet 421™ anti-human CD163
Human peripheral blood monocytes were stained with CD163 (cl... -
PE/Cyanine7 anti-human CD163
Human peripheral blood monocytes were stained with CD163 (cl... -
Brilliant Violet 605™ anti-human CD163
Human peripheral blood monocytes were stained with CD163 (cl... -
FITC anti-human CD163
Human peripheral blood monocytes were stained with CD163 (cl... -
Alexa Fluor® 647 anti-human CD163
Human peripheral blood monocytes were stained with CD163 (cl... Confocal image of human lymph node sample acquired using the... -
APC/Cyanine7 anti-human CD163
Human peripheral blood monocytes were stained with CD163 (cl... -
PE/Dazzle™ 594 anti-human CD163
Human peripheral blood monocytes were stained with CD163 (cl... -
Brilliant Violet 510™ anti-human CD163
Human peripheral blood monocytes were stained with CD163 (cl... -
Brilliant Violet 711™ anti-human CD163
Human peripheral blood monocytes were stained with CD163 (cl... -
Brilliant Violet 785™ anti-human CD163
Human peripheral blood monocytes were stained with CD163 (cl... -
APC/Fire™ 750 anti-human CD163
Human lysed whole blood was stained with CD163 (clone GHI/61... -
TotalSeq™-A0358 anti-human CD163
-
TotalSeq™-C0358 anti-human CD163
-
TotalSeq™-B0358 anti-human CD163
-
PE/Cyanine5 anti-human CD163
Human peripheral blood monocytes were stained with anti-huma... -
TotalSeq™-D0358 anti-human CD163
-
Alexa Fluor® 700 anti-human CD163
Human peripheral blood monocytes were stained with anti-huma... -
Brilliant Violet 650™ anti-human CD163
Human peripheral blood cells were stained with anti-human CD...
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