- Clone
- HI264 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- VII 70312
- Other Names
- PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein, Glycophorin A, MNS blood group, GYPA
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![HI264_PB_052312 HI264_PB_052312](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/HI264_PB_052312.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human red blood cells stained with anti-human CD235a (clone HI264) Pacific Blue™ (filled histogram) or mouse IgG2a, κ Pacific Blue™ isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
349107 | 25 µg | 147 CHF | ||||
349108 | 100 µg | 323 CHF |
CD235a (Glycophorin A) is member of the glycophorin A family. It is a type I sialoglycoprotein with a molecular weight of 10 kD, present in the cell membrane as a homodimer. Glycophorin A is expressed by erythroid precursors and erythrocytes. It carries the antigen determinants for the MNS blood groups and has been proposed to be an inhibitor of hemagglutination and hemolysis. Glycophorin A binds siglec 5, the erythrocyte binding antigen (EBA-175) of P. falciparum and some viruses, including influenza virus and hepatitis A virus.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Violet Laser (405 nm)
-
Application References
(PubMed link indicates BioLegend citation) -
- Mason D, et al. Eds. 2002. Leucocyte Typing VII:White Cell Differentiation Antigens. Oxford University Press. (FC)
- Product Citations
-
- RRID
-
AB_11219199 (BioLegend Cat. No. 349107)
AB_11218988 (BioLegend Cat. No. 349108)
Antigen Details
- Structure
- Member of the glycophorin A family; type I protein with a molecular weight of 10 kD; present in the membrane as homodimer
- Distribution
-
Erythrocytes and erythroid precursors
- Function
- Possible inhibitor of hemagglutination and hemolysis; carries the antigen determinants for the MNS blood groups
- Ligand/Receptor
- Siglec 5, erythrocyte binding antigen (EBA-175) of P. falciparum, receptor for the influenza virus, hepatitis A virus
- Cell Type
- Erythrocytes
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Reid ME. 2009. Immunohematology 25:95.
2. Palacajornsuk P. 2006. Immunohematology 22:171.
3. Pasvol G. 2003. Trends Parasitol. 19:430.
4. Takakuwa Y. 2001. Curr. Opin. Hematol. 8:80. - Gene ID
- 2993 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- CD235a
- Specificity Alt (DOES NOT SHOW ON TDS):
- CD235a
- App Abbreviation (DOES NOT SHOW ON TDS):
- FC
- UniProt
- View information about CD235a on UniProt.org
Related Pages & Pathways
Pages
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Pacific Blue™ anti-human CD235a (Glycophorin A)
Human red blood cells stained with anti-human CD235a (clone ... -
PE anti-human CD235a (Glycophorin A)
Human red blood cells stained with HI264 PE -
Purified anti-human CD235a (Glycophorin A)
Human red blood cells stained with purified HI264 conjugated... -
FITC anti-human CD235a (Glycophorin A)
Human red blood cells stained with HI264 FITC -
PerCP/Cyanine5.5 anti-human CD235a (Glycophorin A)
Human red blood cells were stained with anti-human CD235a (c... -
PE/Cyanine7 anti-human CD235a (Glycophorin A)
Human red blood cells were stained with anti-human CD235a (c... -
APC anti-human CD235a (Glycophorin A)
Human red blood cells were stained with anti-human CD235a (c... -
APC/Cyanine7 anti-human CD235a (Glycophorin A)
Human red blood cells were stained with anti-human CD235a (c... -
TotalSeq™-A0574 anti-human CD235a (Glycophorin A)
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PE/Dazzle™ 594 anti-human CD235a (Glycophorin A)
Human red blood cells were stained with CD235a (Glycophorin ... -
TotalSeq™-C0574 anti-human CD235a (Glycophorin A)
-
Alexa Fluor® 700 anti-human CD235a (Glycophorin A)
Human red blood cells were stained with anti-human CD235a (c... -
TotalSeq™-D0574 anti-human CD235a (Glycophorin A)
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Alexa Fluor® 647 anti-human CD235a (Glycophorin A)
Human red blood cells were stained with anti-human CD235a (G... -
Biotin anti-human CD235a (Glycophorin A)
Human red blood cells were stained with biotinylated anti-hu... -
Brilliant Violet 421™ anti-human CD235a (Glycophorin A)
Human red blood cells were stained with anti-human CD235a (G... -
Spark UV™ 387 anti-human CD235a (Glycophorin A)
Human red blood cells stained with anti-human CD235a (clone ... -
TotalSeq™-B0574 anti-human CD235a (Glycophorin A)
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