- Clone
- W17149D (See other available formats)
- Regulatory Status
- RUO
- Other Names
- B7-1, B7, BB1
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W17149D_PerCPCyanine55_CD80_Antibody_030221 W17149D_PerCPCyanine55_CD80_Antibody_030221](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W17149D_PerCPCyanine55_CD80_Antibody_030221.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Raji cell line was stained with anti-human CD80 (clone W17149D) PerCP/Cyanine5.5 (filled histogram) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
375411 | 25 tests | 171 CHF | ||||
375412 | 100 tests | 375 CHF |
CD80, also known as B7-1, B7, and BB1, is a 60 kD single chain type I glycoprotein belonging to the immunoglobulin superfamily. CD80 is expressed on activated B and T cells, macrophages, and dendritic cells. CD80 binds to CD28 and CD152 (CTLA-4). Along with CD86, CD80 plays a critical role in regulation of T cell activation. The interaction of CD80 with CD28 provides a potent costimulatory signal for T cell activation through the CD3 complex, while its interaction with CTLA-4 provides an inhibitory signal for T cell activation.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- RBL1 cells stably transfected with human CD80 (full length)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. - Excitation Laser
-
Blue Laser (488 nm)
- RRID
-
AB_2890821 (BioLegend Cat. No. 375411)
AB_2890821 (BioLegend Cat. No. 375412)
Antigen Details
- Structure
- Ig superfamily, type I transmembrane glycoprotein, 60 kD
- Distribution
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Activated B cells and T cells, macrophages, and dendritic cells
- Function
- T cell costimulation
- Ligand/Receptor
- CD28, CD152 (CTLA-4)
- Cell Type
- B cells, Dendritic cells, Macrophages, T cells, Tregs
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Lim TS, et al. 2012. PLoS One. 7(9): e45185.
2. Kamphorst, Alice O, et al. 2017. Science. 31;355(6332):1423-1427.
3. Zheng Y, et al. 2004. J. Immunol. 1;172(5):2778-84. - Gene ID
- 941 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- CD80
- Specificity Alt (DOES NOT SHOW ON TDS):
- CD80
- App Abbreviation (DOES NOT SHOW ON TDS):
- FC
- UniProt
- View information about CD80 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
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PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
Other Formats
View All CD80 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD80 Antibody | W17149D | FC |
APC anti-human CD80 Antibody | W17149D | FC |
FITC anti-human CD80 Antibody | W17149D | FC |
PE/Cyanine7 anti-human CD80 | W17149D | FC |
PE anti-human CD80 | W17149D | FC |
PerCP/Cyanine5.5 anti-human CD80 | W17149D | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD80 Antibody
Human B-cell Burkitt's lymphoma cell line Raji was stained w... Peripheral blood mononuclear cells were cultured in the pres... -
APC anti-human CD80 Antibody
Raji cell line was stained with anti-human CD80 (clone W1714... -
FITC anti-human CD80 Antibody
Raji cell line was stained with anti-human CD80 (clone W1714... -
PE/Cyanine7 anti-human CD80
Raji cell line was stained with anti-human CD80 (clone W1714... -
PE anti-human CD80
Raji cell line was stained with anti-human CD80 (clone W1714... -
PerCP/Cyanine5.5 anti-human CD80
Raji cell line was stained with anti-human CD80 (clone W1714...
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