- Clone
- A15115A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Synuclein alpha-140, non-A4 component of amyloid, alpha-synuclein, isoform NACP140, non-amyloid beta component (NAP), PARK1, NACP, PD1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A15115A_Purified_alpha_synuclein_Antibody_1_012122 A15115A_Purified_alpha_synuclein_Antibody_1_012122](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A15115A_Purified_alpha_synuclein_Antibody_1_012122.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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IHC staining of purified anti-α-Synuclein, 80-96 antibody (clone A15115A) on formalin-fixed paraffin-embedded Parkinson's disease human brain tissue. Following antigen retrieval using 70% formic acid for 20 minutes at room temperature, the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. -
Western blot of purified anti-α-Synuclein, 80-96 antibody (clone A15115A), and isotype-matched IgG1 control, demonstrating the binding specificity of clone A15115A to endogenous and recombinant human α-synuclein. Lane 1: 50 ng of recombinant human α-synuclein; Lane 2: 50 ng of recombinant human pS87 α-synuclein; Lane 3: 25 µg of normal human brain lysate. Blots were incubated with 1.0 µg/mL of each primary antibody at room temperature for two hours, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. Enhanced chemiluminescence was used as the detection system. -
Direct ELISA of purified anti-α-Synuclein antibody, 80-96 (clone A15115A), binding to plate-immobilized recombinant human unmodified and pS87 α-synuclein. ELISA was performed by coating wells with 150 ng of each recombinant α-synuclein protein for two hours at 37°C. The wells were then incubated with the primary antibody at 37°C for one hour, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. 3, 3', 5, 5' tetramethylbenzidine (TMB) was used as the detection system.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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848301 | 25 µg | 89 CHF | ||||
848302 | 100 µg | 218 CHF |
Alpha-synuclein is expressed principally in the nervous system, but it is also produced in other tissues, including the skin. In the brain, the protein is primarily neuronal, but it is also present in glia. Neuronal α-synuclein is concentrated in presynaptic nerve terminals, interacts with plasma membrane phospholipids, and is also present in nuclei and mitochondria. At least three isoforms of α-synuclein are produced through alternative splicing. The most common isoform is a 140 amino acid-long transcript. Other isoforms include, α-synuclein-126, lacking residues 41-54; and α-synuclein-112, which lacks residues 103-130. α-synuclein’s physiological role is poorly understood, but the protein has been implicated in regulating dopamine release and transport, synaptic vesicle clustering, and functioning as a SNARE-complex chaperone. α-synuclein fibrils are a major component of the intracellular Lewy bodies that are associated with Parkinson's disease, Lewy body dementia, and multiple system atrophy.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- KLH-Conjugated synthetic peptide that included amino acid residues 80-96 of human α-synuclein.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
Direct ELISA, WB - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 2.0 - 10 µg/ml is suggested. For Direct ELISA, the suggested use of this reagent is 0.02 - 3.0 per ml. For Western blotting, the suggested use of this reagent is 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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This clone does not cross-react with ß or ?-synucleins using ELISA and WB as detection method.
- Product Citations
-
- RRID
-
AB_2650687 (BioLegend Cat. No. 848301)
AB_2650688 (BioLegend Cat. No. 848302)
Antigen Details
- Structure
- α-synuclein's canonical isoform is a 140 amino acid protein with an apparent molecular mass of 14 kD.
- Distribution
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Tissue distribution: Primarily nervous system, but lower expression in other tissues such as blood and skin.
Cellular distribution: Cytoskeleton, cytosol, lysosome, mitochondria, nucleus, plasma membrane, and extracellular. - Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Molecular Family
- α-Synuclein, Phospho-Proteins
- Antigen References
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1. Mor DE, et al. 2016. Neurobiol Dis. 88:66. PubMed
2. Jucker M & Walker LC. 2013. Nature. 501(7465):45. PubMed
3. Bartels T, et al. 2011. Nature. 477(7362): 107. PubMed
4. Devine MJ, et al. 2011. Mov Disord. 26:2160. PubMed - Gene ID
- 6622 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- alpha-Synuclein 80-96
- Specificity Alt (DOES NOT SHOW ON TDS):
- α-Synuclein, 80-96
- App Abbreviation (DOES NOT SHOW ON TDS):
- IHC-P,Direct ELISA,WB
- UniProt
- View information about alpha-Synuclein 80-96 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All α-Synuclein, 80-96 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-α-Synuclein, 80-96 | A15115A | IHC-P,Direct ELISA,WB |
HRP anti-α-Synuclein, 80-96 | A15115A | WB,IHC-P |
Biotin anti-α-Synuclein, 80-96 | A15115A | WB,IHC-P |
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Purified anti-α-Synuclein, 80-96
IHC staining of purified anti-α-Synuclein, 80-96 antibody (c... Western blot of purified anti-α-Synuclein, 80-96 antibody (c... Direct ELISA of purified anti-α-Synuclein antibody, 80-96 (c... -
HRP anti-α-Synuclein, 80-96
Western blot of HRP anti-α-Synuclein, 80-96 antibody (clone ... IHC staining of HRP anti-α-Synuclein, 80-96 antibody (clone ... -
Biotin anti-α-Synuclein, 80-96
Western blot of Biotin anti-α-Synuclein, 80-96 antibody (clo... IHC staining of Biotin anti-α-Synuclein, 80-96 antibody (clo...
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