- Clone
- 1GFP63 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Green fluorescent protein, GFP
- Isotype
- Mouse IgG2a
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1GFP63_Purified_GFP_Antibody_031519_updated.png 1GFP63_Purified_GFP_Antibody_031519_updated.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/1GFP63_Purified_GFP_Antibody_031519_updated.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Total cell lysates (15 µg total protein) from mock-transfected 293E and 293E transfected with a plasmid encoding a GFP-fused protein were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-GFP Antibody, clone 1GFP63, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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668206 | 25 µg | 89 CHF | ||||
668205 | 100 µg | 136 CHF |
Green fluorescent protein (GFP) was originally identified as a protein involved in bioluminescence, which is from the jellyfish Aequorea victoria. It is widely used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces without the need for exogenous substrates or cofactors, or other intrinsic or extrinsic proteins. This makes GFP a useful tool for monitoring gene expression and protein localization in vivo. Purified GFP is a 27 kD monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.
Product DetailsProduct Details
- Verified Reactivity
- Aequorea victoria, Epitope tag
- Reported Reactivity
- Species independent
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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WB - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
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- RRID
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AB_2749908 (BioLegend Cat. No. 668206)
AB_2616789 (BioLegend Cat. No. 668205)
Antigen Details
- Biology Area
- Cell Biology
- Antigen References
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1. Ishikura H, et al. 2004. Anticancer Res. 24:719.
2. Rizzuto R, et al. 1996. Curr. Biol. 6:183.
3. Chalfie M, et al. 1994. Science 263:802. - Gene ID
- NA
- Specificity (DOES NOT SHOW ON TDS):
- GFP
- Specificity Alt (DOES NOT SHOW ON TDS):
- GFP
- App Abbreviation (DOES NOT SHOW ON TDS):
- WB
- UniProt
- View information about GFP on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All GFP Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-GFP | 1GFP63 | WB |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-GFP
Total cell lysates (15 µg total protein) from mock-transfect...
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