- Clone
- A21005D (See other available formats)
- Regulatory Status
- RUO
- Other Names
- retinoic acid receptor (RAR)-related orphan receptor gamma T, ROR gamma2, RAR Related Orphan Receptor C, TOR, Retinoic Acid-Binding Receptor Gamma, IMD42, NR1f3
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![a. A21005D_PURE_RORgammat_Antibody-110822 a. A21005D_PURE_RORgammat_Antibody-110822](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A21005D_PURE_RORgammat_Antibody-110822.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole cell extracts (15 µg total protein) from SR cells, Jurkat cells, mouse thymus, and mouse spleen were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL of purified anti-RORγt (clone A21005D) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control at a 1:10,000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker -
SR cells (positive control, filled histogram) and Jurkat cells (negative control, open histogram) were fixed and permeabilized using True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) and intracellularly stained with purified anti-RORγt (clone A21005D) or purified mouse IgG1, κ isotype control (Cat. No. 401402) (open histogram, dashed line) followed by PE goat anti-mouse IgG (Cat. No. 405307). -
ICC staining of purified anti-RORγt (clone A21005D) on SR cells (positive control, left panel) and Jurkat cells (negative control, right panel). The cells were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) and permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. The cells were then stained with 5.0 µg/mL of purified anti-RORγt (clone A21005D), followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801), and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 60X objective. Scale bar: 50 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
609051 | 25 µg | 118 CHF | ||||
609052 | 100 µg | 347 CHF |
RORγt is a member of the nuclear hormone receptor family of transcription factors. RORγt is the isoform 2 of the RORC gene. It is produced by alternative splicing where the two 5’-exons are replaced by an alternative exon downstream in the gene, this splicing causes a shorter protein carrying three distinctive amino acids at its N-terminal end which are not present on RORγ. RORγt is mainly expressed on immature CD4+/CD8+ thymocytes and Th17 cells. It is important on lymphoid organogenesis in lymph nodes and Peyer’s patches but not on spleen. It plays an important role in promoting differentiation of pro-inflammatory Th17 cells by reducing apoptosis.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide from human RORγt
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICFC, ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 µg/mL. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody does not cross react with mouse RORγ or RORγt.
- RRID
-
AB_2927967 (BioLegend Cat. No. 609051)
AB_2927967 (BioLegend Cat. No. 609052)
Antigen Details
- Structure
- RORγt is a 497 amino acid protein with a predicted molecular weight of 56 kD.
- Distribution
-
Expressed on different subsets of T cells (mature and immature), on thymus and lymph nodes. Also, it has been found expressed on Treg cells in colon.
- Function
- Promotion of pro-inflammatory Th17 cells by downregulation of Fas ligand and IL2 which reduce apoptosis.
- Ligand/Receptor
- Ligand are cholesterol metabolites
- Cell Type
- Lymphocytes, Macrophages, NK cells, NKT cells, T cells, Th17, Tregs
- Biology Area
- Adaptive Immunity, Immuno-Oncology, Immunology, Innate Immunity, Transcription Factors
- Molecular Family
- Innate Immune Signaling
- Antigen References
-
- Castro G, et al. 2017. PloS One. 12: e0181868.
- Villey I, et al. 1999. Eur J Immunol. 29:4072-80.
- Lamb D, et al. 2021. Respir Res. 22:158.
- Gene ID
- 6097 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- RORgammat
- Specificity Alt (DOES NOT SHOW ON TDS):
- RORγt
- App Abbreviation (DOES NOT SHOW ON TDS):
- WB,ICC,ICFC
- UniProt
- View information about RORgammat on UniProt.org
Other Formats
View All RORγt Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-RORγt | A21005D | WB,ICC,ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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