- Clone
- 63D3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Monocyte differentiation antigen CD14, myeloid cell-specific leucine-rich glycoprotein, LPS receptor
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![63D3_BV510_CD14_Antibody_100217 63D3_BV510_CD14_Antibody_100217](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/63D3_BV510_CD14_Antibody_100217.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood monocytes were stained with Brilliant Violet™ 510 anti-human CD14 (clone 63D3, closed histogram) or Brilliant Violet™ 510 mouse IgG1, κ isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
367123 | 25 tests | 198 CHF | ||||
367124 | 100 tests | 402 CHF |
CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein that is also known as the LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokine expression in monocytes and neutrophils.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Purified human peripheral blood monocytes.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
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Application References
(PubMed link indicates BioLegend citation) -
- Fridlender ZG, et al. 1999. Hum. Immunol. 11:1028.
- Devitt A, et al. 1998. Nature 6675:505.
- Product Citations
-
- RRID
-
AB_2716228 (BioLegend Cat. No. 367123)
AB_2716229 (BioLegend Cat. No. 367124)
Antigen Details
- Structure
- GPI-linked membrane glycoprotein.
- Distribution
-
Monocytes, macrophages, dendritic cells, and granulocytes.
- Function
- LPS receptor, clearance of Gram-negative pathogens.
- Interaction
- LPS.
- Ligand/Receptor
- LPS.
- Cell Type
- Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
- Stocks SC, et al. 1990. Biochem. J. 268:275.
- Wright SD, et al. 1990. Science 4975:1431.
- Gene ID
- 929 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- CD14
- Specificity Alt (DOES NOT SHOW ON TDS):
- CD14
- App Abbreviation (DOES NOT SHOW ON TDS):
- FC
- UniProt
- View information about CD14 on UniProt.org
Related FAQs
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clo... -
PE anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clo... -
Biotin anti-human CD14
Human peripheral blood monocytes were stained with biotinyla... -
APC/Cyanine7 anti-human CD14
Human peripheral blood monocytes stained with CD14 (clone 63... -
PE/Cyanine7 anti-human CD14
Human peripheral blood monocytes were stained with PE/Cyanin... -
PerCP/Cyanine5.5 anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clo... -
Alexa Fluor® 700 anti-human CD14
Human peripheral blood monocytes were stained with Alexa Flu... -
APC anti-human CD14
Human peripheral blood monocytes were stained with APC anti-... -
FITC anti-human CD14
Human peripheral blood monocytes were stained with FITC anti... -
APC/Fire™ 750 anti-human CD14
Human peripheral blood monocytes were stained with MERTK PE ... -
Pacific Blue™ anti-human CD14
Human peripheral blood monocytes were stained with Pacific B... -
Brilliant Violet 510™ anti-human CD14
Human peripheral blood monocytes were stained with Brilliant... -
Brilliant Violet 605™ anti-human CD14
Human peripheral blood monocytes were stained with Brilliant... -
Alexa Fluor® 647 anti-human CD14
Human peripheral blood monocytes were stained with Alexa Flo... -
Alexa Fluor® 488 anti-human CD14
Human peripheral blood monocytes were stained with Alexa Flo... -
TotalSeq™-A0051 anti-human CD14
-
PE/Dazzle™ 594 anti-human CD14
Human peripheral blood monocytes were stained with PE/Dazzle... -
Brilliant Violet 750™ anti-human CD14
Human peripheral blood monocytes were stained with Brilliant... -
TotalSeq™-C0051 anti-human CD14
-
Brilliant Violet 421™ anti-human CD14
Human peripheral blood monocytes were stained with Brilliant... Human peripheral blood monocytes were stained with HLA-DR FI... -
Brilliant Violet 785™ anti-human CD14
Human peripheral blood monocytes were stained with Brilliant... Human peripheral blood monocytes were stained with HLA-DR FI... -
Brilliant Violet 711™ anti-human CD14
Human peripheral blood monocytes were stained with HLA-DR FI... Human peripheral blood monocytes were stained with Brilliant... -
TotalSeq™-B0051 anti-human CD14
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Spark Blue™ 550 anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clo... -
Spark NIR™ 685 anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clo... -
PE/Fire™ 640 anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clo... -
APC/Fire™ 810 anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clo... -
PerCP anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clo... -
PE/Fire™ 700 anti-human CD14
Human peripheral blood monocytes were stained with anti-huma... -
Spark YG™ 581 anti-human CD14
Human peripheral blood monocytes were stained with anti-huma... -
Spark Blue™ 574 anti-human CD14
Human peripheral blood monocytes were stained with anti-huma... -
Spark Red™ 718 anti-human CD14 (Flexi-Fluor™)
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