- Clone
- ASL-32 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CEACAM1/5/6, BGP, NCA
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![ASL-32_PE_051409.jpg ASL-32_PE_051409.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/ASL-32_PE_051409.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human colorectal adenocarcinoma cell line HT29 stained with purified ASL-32 conjugated to PE -
Human paraffin-embedded colon tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH7.4) at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/ml of purified anti-human CD66a/c/e (clone ASL-32) antibody overnight at 4°C. On the next day, the tissue was incubated with Alexa Fluor® 594 Goat anti-mouse IgG (clone Poly4053) antibody (red) for an hour. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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342302 | 100 µg | 141 CHF |
The ASL-32 mAb reacts with an antigen epitope shared by CD66a, c and e. CD66a/c/e are members of the CEA (carcinoembryonic antigen) family of the Ig superfamily. CD66 plays a role in hemophilic and heterophilic adhesion. CD66a, also known as CEACAM1 and BGP (biliary glycoprotein), is mainly expressed on granulocytes, binds to CD66a, c, e, and CD62E. CD66c (known as CEACAM6, NCA) is expressed on granulocytes and epithelial cells. The ligands of CD66c are CD66a-e, CD62E and Galectins. CD66e molecule is also called CEA or CEACAM5, and is primarily found on epithelial cells. CD66e binds to CD66a, c and e.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
IHC-P - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µl volume. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
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Application References
(PubMed link indicates BioLegend citation) -
- Shey MS, et al. 2012. J Immunol Methods. 376:79. PubMed.
- Product Citations
-
- RRID
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AB_1626265 (BioLegend Cat. No. 342302)
Antigen Details
- Structure
- Member of the CEA (carcinoembryonic antigen) family of the Ig superfamily
- Distribution
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Granulocytes, epithelial cells
- Function
- Homophilic and heterophilic adhesion
- Ligand/Receptor
- CD66a-e, CD62E, galectins
- Cell Type
- Epithelial cells, Granulocytes
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiley-Liss. A John Wiley & Sons Inc. Publication
- Gene ID
- 634 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- CD66a CD66c CD66e
- Specificity Alt (DOES NOT SHOW ON TDS):
- CD66a/c/e
- App Abbreviation (DOES NOT SHOW ON TDS):
- FC,IHC-P
- UniProt
- View information about CD66a CD66c CD66e on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All CD66a/c/e Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD66a/c/e | ASL-32 | FC,IHC-P |
PE anti-human CD66a/c/e | ASL-32 | FC |
Alexa Fluor® 488 anti-human CD66a/c/e | ASL-32 | FC |
APC anti-human CD66a/c/e | ASL-32 | FC |
PE/Cyanine7 anti-human CD66a/c/e | ASL-32 | FC |
PerCP/Cyanine5.5 anti-human CD66a/c/e | ASL-32 | FC |
Brilliant Violet 421™ anti-human CD66a/c/e | ASL-32 | FC,IHC-P |
Alexa Fluor® 594 anti-human CD66a/c/e | ASL-32 | IHC-P |
Alexa Fluor® 647 anti-human CD66a/c/e | ASL-32 | FC,IHC-P |
TotalSeq™-A0188 anti-human CD66a/c/e | ASL-32 | PG |
APC/Fire™ 750 anti-human CD66a/c/e | ASL-32 | FC |
Brilliant Violet 605™ anti-human CD66a/c/e | ASL-32 | FC |
TotalSeq™-C0188 anti-human CD66a/c/e | ASL-32 | PG |
Spark YG™ 570 anti-human CD66a/c/e | ASL-32 | IHC-P,FC |
TotalSeq™-B0188 anti-human CD66a/c/e | ASL-32 | PG |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD66a/c/e
Human colorectal adenocarcinoma cell line HT29 stained with ... Human paraffin-embedded colon tissue slices were prepared wi... -
PE anti-human CD66a/c/e
Human colorectal adenocarcinoma cell line HT29 stained with ... -
Alexa Fluor® 488 anti-human CD66a/c/e
Human colorectal adenocarcinoma cell line HT-29 stained with... -
APC anti-human CD66a/c/e
Human colorectal adenocarcinoma cell line HT-29 was stained ... Human peripheral blood granulocytes were stained with CD66a/... -
PE/Cyanine7 anti-human CD66a/c/e
Human colorectal adenocarcinoma cell line HT-29 was stained ... -
PerCP/Cyanine5.5 anti-human CD66a/c/e
Human colorectal adenocarcinoma cell line HT-29 was stained ... -
Brilliant Violet 421™ anti-human CD66a/c/e
Human colorectal adenocarcinoma cell line HT-29 was stained ... Human paraffin-embedded spleen tissue slices were prepared w... -
Alexa Fluor® 594 anti-human CD66a/c/e
Human paraffin-embedded colon tissue slices were prepared wi... -
Alexa Fluor® 647 anti-human CD66a/c/e
Human colorectal adenocarcinoma cell line HT-29 was stained ... Human paraffin-embedded spleen tissue slices were prepared w... -
TotalSeq™-A0188 anti-human CD66a/c/e
-
APC/Fire™ 750 anti-human CD66a/c/e
Human peripheral blood granulocytes were stained with CD66a/... -
Brilliant Violet 605™ anti-human CD66a/c/e
Human peripheral blood granulocytes were stained with CD66a/... -
TotalSeq™-C0188 anti-human CD66a/c/e
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Spark YG™ 570 anti-human CD66a/c/e
Human paraffin-embedded spleen tissue slices were prepared w... Human paraffin-embedded colon tissue slices were prepared wi... Human colorectal adenocarcinoma cell line HT-29 was stained ... -
TotalSeq™-B0188 anti-human CD66a/c/e
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