- Clone
- 17A2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T cell antigen receptor complex, T3
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![17A2_LEAF_021606.jpg 17A2_LEAF_021606.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/17A2_LEAF_021606.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes stained with Ultra-LEAF™ purified CD3 (clone 17A2), followed by anti-rat IgG FITC.
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- γδTCR-positive T-T hybridoma D1
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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FC - Quality tested
IHC-F, IP, ICC - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.
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Application References
(PubMed link indicates BioLegend citation) - Product Citations
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- RRID
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AB_2810316 (BioLegend Cat. No. 100255)
AB_2810315 (BioLegend Cat. No. 100254)
AB_2810317 (BioLegend Cat. No. 100256)
AB_2810313 (BioLegend Cat. No. 100239)
AB_2561487 (BioLegend Cat. No. 100238)
AB_2810314 (BioLegend Cat. No. 100253)
Antigen Details
- Structure
- Ig superfamily, CD3/TCR, 20 kD
- Distribution
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Thymocytes (differentiation dependent), mature T cells, NK-T cells
- Function
- Antigen recognition, TCR signal transduction, T cell activation
- Ligand/Receptor
- Peptide antigen/MHC-complex
- Antigen References
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1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263. - Gene ID
- 12502 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- CD3
- Specificity Alt (DOES NOT SHOW ON TDS):
- CD3
- App Abbreviation (DOES NOT SHOW ON TDS):
- FC,IHC-F,IP,ICC
- UniProt
- View information about CD3 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD3 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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FITC anti-mouse CD3
C57BL/6 splenocytes stained with 17A2 FITC and 145-2C11 PE -
PE anti-mouse CD3
C57BL/6 mouse splenocytes stained with 17A2 PE -
Purified anti-mouse CD3
C57BL/6 mouse splenocytes stained with purified 17A2, follow... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... Fresh, frozen mouse spleen was stained with purified CD3 clo... -
Alexa Fluor® 647 anti-mouse CD3
C57BL/6 mouse splenocytes stained with 17A2 Alexa Fluor® 647 Dissected C57/B6 mouse spleen was immersed in 4% paraformal... Formalin-fixed, 400 micron-thick mouse spleen section was bl... Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen secti... -
Alexa Fluor® 488 anti-mouse CD3
C57BL/6 mouse splenocytes stained with 17A2 Alexa Fluor® 488 C57BL/6 mouse frozen spleen section was fixed with 4% parafo... Formalin-fixed, 300 micron-thick mouse spleen section was bl... Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen secti... -
Pacific Blue™ anti-mouse CD3
C57BL/6 mouse splenocytes stained with Pacific Blue™ 1... -
Alexa Fluor® 700 anti-mouse CD3
C57BL/6 mouse splenocytes stained with 17A2 Alexa Fluor® 700 -
PerCP/Cyanine5.5 anti-mouse CD3
C57BL/6 splenocytes were stained with CD45R/B220 APC and CD3... -
PE/Cyanine7 anti-mouse CD3
C57BL/6 splenocytes stained with 17A2 PE/Cyanine7 -
APC/Cyanine7 anti-mouse CD3
C57BL/6 splenocytes were stained with CD45R/B220 FITC and CD... -
Brilliant Violet 421™ anti-mouse CD3
C57BL/6 mouse splenocytes were stained with CD19 APC and CD3... C57BL/6 mouse splenocytes were fixed with 2% paraformaldehyd... -
Brilliant Violet 570™ anti-mouse CD3
C57BL/6 mouse splenocytes were stained with CD19 APC and CD3... -
Brilliant Violet 650™ anti-mouse CD3
C57BL/6 mouse splenocytes were stained with CD19 APC and CD3... -
Brilliant Violet 785™ anti-mouse CD3
C57BL/6 mouse splenocytes were stained with CD19 APC and CD3... -
Brilliant Violet 510™ anti-mouse CD3
C57BL/6 mouse splenocytes were stained with CD3 (clone 17A2)... -
APC anti-mouse CD3
C57BL/6 mouse splenocytes were stained with CD3 (clone 17A2)... -
Ultra-LEAF™ Purified anti-mouse CD3
C57BL/6 mouse splenocytes stained with Ultra-LEAF™ purified ... -
Brilliant Violet 605™ anti-mouse CD3
C57BL/6 mouse splenocytes were stained with CD19 APC and CD3... -
Alexa Fluor® 594 anti-mouse CD3
C57BL/6 mouse splenocytes were stained with CD3 (clone 17A2)... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... Formalin-fixed, 300 micron-thick mouse spleen section was bl... Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen secti... Mice were injected subcutaneously with sheep red blood cells... -
Brilliant Violet 711™ anti-mouse CD3
C57BL/6 splenocytes were stained with CD19 APC and CD3 (clon... -
Biotin anti-mouse CD3
C57BL/6 splenocytes were stained with CD19 APC and biotinyla... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
PE/Dazzle™ 594 anti-mouse CD3
C57BL/6 mouse splenocytes were stained with CD3 (clone 17A2)... -
APC/Fire™ 750 anti-mouse CD3
C57BL/6 splenocytes were stained with CD45R/B220 PE and CD3 ... -
Brilliant Violet 750™ anti-mouse CD3
C57BL/6 mouse splenocytes were stained with CD3 (clone 17A2)... -
TotalSeq™-A0182 anti-mouse CD3
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TotalSeq™-B0182 anti-mouse CD3
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Spark Blue™ 550 anti-mouse CD3
C57BL/6 mouse splenocytes were stained with CD3 (clone 17A2)... -
Spark NIR™ 685 anti-mouse CD3
C57BL/6 mouse splenocytes were stained with CD3 (clone 17A2)... -
TotalSeq™-C0182 anti-mouse CD3
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APC/Fire™ 810 anti-mouse CD3
C57BL/6 splenocytes were stained with CD45R/B220 PE and CD3 ... -
PE/Fire™ 640 anti-mouse CD3
C57BL/6 splenocytes were stained with CD45R/B220 APC and CD3... -
Spark YG™ 570 anti-mouse CD3
C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
PE/Fire™ 700 anti-mouse CD3
C57BL/6 splenocytes were stained with CD45R/B220 APC and CD3... -
PE/Cyanine5 anti-mouse CD3
C57BL/6 splenocytes were stained with anti-mouse/human CD45R... -
Spark Blue™ 574 anti-mouse CD3 Antibody
C57BL/6 splenocytes were stained with anti-mouse CD45R/B220 ... -
Spark Violet™ 423 anti-mouse CD3
C57BL/6 splenocytes were stained with anti-mouse CD45R/B220 ... -
PE/Fire™ 810 anti-mouse CD3
C57BL/6 splenocytes were stained with anti-mouse/human CD45R... -
Spark Red™ 718 anti-mouse CD3
C57BL/6 splenocytes were stained with anti-mouse/human CD45R... -
Spark UV™ 387 anti-mouse CD3
C57BL/6 splenocytes were stained with anti-mouse CD45R/B220 ...
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