- Clone
- OX-114 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Basigin
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![OX-114_A488_CD147_Antibody_061318 OX-114_A488_CD147_Antibody_061318](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/OX-114_A488_CD147_Antibody_061318.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse frozen testis section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 2.5 µg/mL of anti-mouse CD147 (clone OX-114) Alexa Fluor® 488 (green) and anti-mouse CD106 (clone 429 (MVCAM.A)) Alexa Fluor® 594 (red) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective. -
Dissected C57/B6 mouse testis was immersed in 4% paraformaldehyde (PFA) overnight followed by 30% sucrose immersion overnight and frozen in OCT. Frozen section was blocked with 5% FBS and 5% mouse serum for 30 minutes at room temperature. Then the tissue section was stained with 5 µg/mL of anti-mouse CD147 (clone OX-114) Alexa Fluor® 488 (green) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured by 20X objective. -
Formalin-fixed, 300 micron-thick mouse testis section was blocked, permeabilized and stained overnight with CD106 (clone 429, MCVAM.A) Alexa Fluor® 647 (red), and CD147 (clone OX-114) Alexa Fluor® 488 (green) both at 5 µg/mL, optically cleared, and analyzed at 225 µm imaging depth on a confocal microscope. Watch the video. -
Paraformaldehyde-fixed (4%), 500 µm-thick mouse testis section was processed according to the Ce3D™ Tissue Clearing Kit protocol (Cat. No. 427701). The section was costained with anti-mouse CD147 Antibody (clone OX-114) Alexa Fluor® 488 at 5 µg/mL (green), anti-mouse CD117 (c-Kit) Antibody (clone 2B8) Alexa Fluor® 594 at 5 µg/mL (yellow), and anti-mouse CD106 Antibody (clone 429 (MVCAM.A)) Alexa Fluor® 647 at 5 µg/mL (magenta) and counterstained with DAPI (blue). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
Watch the video.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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123717 | 25 µg | 147 CHF | ||||
123718 | 100 µg | 358 CHF |
CD147 is a widely expressed membrane glycoprotein and reported to be implicated in cell adhesion, embryonic development, T cell activation and many other functions. It is expressed in thymocytes and many other cells, including epithelial and endothelial cells.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
IHC-F - Quality tested
3D IHC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.5 - 5.0 µg/mL is suggested. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
Clone OX-114 has been shown to bind both two and three domain forms of mouse CD1471.
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Application References
(PubMed link indicates BioLegend citation) -
- Hanna SM, et al. 2003. BMC Biochemistry 4:17.
- Product Citations
-
- RRID
-
AB_2734189 (BioLegend Cat. No. 123717)
AB_2734190 (BioLegend Cat. No. 123718)
Antigen Details
- Structure
- Single chain type I transmembrane 58 kd heavily glycosylated protein. N terminal extracellular part consists of two C2 type Ig-like domains which are highly glycosylated. Recently reported: isoform with an additional membrane-distal Ig-like domain.
- Distribution
-
CD147 is expressed at varying levels in many cells types, including haematopoietic, epithelial, and endothelial cells. Expressed on immature thymocytes and at varying levels on spleen cells , bone marrow cells and macrophages.
- Function
- CD147 is implicated in multiple functions, such as matrix metalloproteinase induction, cell adhesion, retinal cell development, HIV attachment, embryonic development and T cell activation.
- Ligand/Receptor
- cyclophilin
- Cell Type
- Macrophages, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Hanna SM, et al. 2003. BMC Biochemistry 4:17
2. Yurchenko V, et al. 2005. Immunology 117:301
3. Renno T, et al. 2002. J. Immunol. 168:4946 - Gene ID
- 12215 View all products for this Gene ID
- UniProt
- View information about CD147 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD147 | OX-114 | FC,IHC-F,IP |
FITC anti-mouse CD147 | OX-114 | FC |
PE anti-mouse CD147 | OX-114 | FC |
Alexa Fluor® 594 anti-mouse CD147 | OX-114 | IHC-F |
Alexa Fluor® 647 anti-mouse CD147 | OX-114 | IHC-F |
Alexa Fluor® 488 anti-mouse CD147 | OX-114 | IHC-F,3D IHC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD147
C57BL/6 mouse frozen testis section was fixed with 4% parafo... C57BL/6 mouse frozen testis section was fixed with 4% parafo... -
FITC anti-mouse CD147
C57BL/6 thymocytes stained with OX-114 FITC -
PE anti-mouse CD147
C57BL/6 thymocytes stained with OX-114 PE -
Alexa Fluor® 594 anti-mouse CD147
C57BL/6 mouse frozen testis section was fixed with 4% parafo... -
Alexa Fluor® 647 anti-mouse CD147
C57BL/6 mouse frozen testis section was fixed with 4% parafo... -
Alexa Fluor® 488 anti-mouse CD147
C57BL/6 mouse frozen testis section was fixed with 4% parafo... Dissected C57/B6 mouse testis was immersed in 4% paraformal... Formalin-fixed, 300 micron-thick mouse testis section was bl... Paraformaldehyde-fixed (4%), 500 µm-thick mouse testis secti...
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