- Clone
- YTS156.7.7 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Lyt-3, Ly-3
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![YTS156dot7dot7_A488_CD8b_Antibody_022119 YTS156dot7dot7_A488_CD8b_Antibody_022119](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/YTS156dot7dot7_A488_CD8b_Antibody_022119.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were first treated with TruStain FcX™ (anti-mouse CD16/32) Antibody and True-Stain Monocyte Blocker™. Cells were then stained with CD3ε APC and CD8b (clone YTS156.7.7) Alexa Fluor® 488 (left) or Rat IgG2b, κ Alexa Fluor® 488 isotype control (right). -
C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of anti-mouse CD8b (clone YTS156.7.7) Alexa Fluor® 488 (green), anti-mouse CD3 (clone 17A2) Alexa Fluor® 647 (red) and anti-mouse B220 (clone RA3-6B2) Alexa Fluor® 594 (blue) overnight at 4°C. The image was captured by 10X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
126627 | 25 µg | 124 CHF | ||||
126628 | 100 µg | 311 CHF |
CD8b is the 32 kD β chain of CD8, also known as Lyt-3 or Ly-3. It is a member of the Ig superfamily expressed as a heterodimer with the CD8α chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in T cell activation.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse thymocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
IHC-F - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen tissue sections.
-
Application References
(PubMed link indicates BioLegend citation) -
- McNical AM, et al. 2007. Eur. J. Immunol. 37:1634.
- Product Citations
-
- RRID
-
AB_2800618 (BioLegend Cat. No. 126627)
AB_2800619 (BioLegend Cat. No. 126628)
Antigen Details
- Structure
- Ig superfamily, CD8β chain, 34 kD
- Distribution
-
Majority of thymocytes, cytotoxic T cell subsets
- Function
- Co-receptor of TCR
- Ligand/Receptor
- MHC class I
- Cell Type
- T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1.Barclay A, et al. 1997. The Leukocyte antigen Facts Book Academic Press.
2. Zamoyska R. 1994. Immunity 1:243-246.
3. Ellmeier W, et al. 1999. Annu. Rev Immunol 17:523.
4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503. - Gene ID
- 12526 View all products for this Gene ID
- UniProt
- View information about CD8beta on UniProt.org
Related FAQs
Other Formats
View All CD8b Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD8b
C57BL/6 mouse frozen spleen section was fixed with 4% parafo... C57BL/6 mouse splenocytes stained with purified YTS156.7.7, ... C57BL/6 mouse splenocytes stained with purified YTS156.7.7, ... -
Biotin anti-mouse CD8b
BALB/c mouse splenocytes stained with biotinylated YTS156.7.... -
FITC anti-mouse CD8b
BALB/c mouse splenocytes stained with YTS156.7.7 FITC -
PE anti-mouse CD8b
C57BL/6 mouse splenocytes stained with YTS156.7.7 PE -
PerCP/Cyanine5.5 anti-mouse CD8b
C57BL/6 mouse splenocytes were stained with CD3e APC and CD8... -
Alexa Fluor® 647 anti-mouse CD8b
57BL/6 mouse splenocytes stained with YTS156.7.7 Alexa Fluor... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
APC anti-mouse CD8b
C57BL/6 mouse splenocytes were stained with CD8b (clone YTS1... -
PE/Cyanine7 anti-mouse CD8b
C57BL/6 mouse splenocytes were stained with CD8b (YTS156.7.7... -
Alexa Fluor® 700 anti-mouse CD8b
C57BL/6 mouse splenocyte stained with CD8b (clone YTS156.7.7... -
APC/Cyanine7 anti-mouse CD8b
C57BL/6 mouse splenocytes stained with CD8b (clone YTS156.7.... -
PE/Dazzle™ 594 anti-mouse CD8b
C57BL/6 mouse splenocytes were stained with CD4 FITC and CD8... -
TotalSeq™-A0230 anti-mouse CD8b (Ly-3)
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Brilliant Violet 510™ anti-mouse CD8b (Ly-3)
C57BL/6 mouse splenocytes were first treated with TruStain F... -
Brilliant Violet 711™ anti-mouse CD8b (Ly-3)
C57BL/6 mouse splenocytes were first treated with TruStain F... -
Alexa Fluor® 488 anti-mouse CD8b (Ly-3)
C57BL/6 mouse splenocytes were first treated with TruStain F... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
APC/Fire™ 750 anti-mouse CD8b (Ly-3)
C57BL/6 mouse splenocytes were first treated with TruStain F... -
Brilliant Violet 421™ anti-mouse CD8b (Ly-3)
C57BL/6 mouse splenocytes were first treated with TruStain F... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
Alexa Fluor® 594 anti-mouse CD8b (Ly-3)
C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
TotalSeq™-B0230 anti-mouse CD8b (Ly-3)
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TotalSeq™-C0230 anti-mouse CD8b (Ly-3)
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