- Clone
- RMG2b-1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Immunoglobulin G2b
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![RMG2b-1_A488_IgG2b_Antibody_1_062620 RMG2b-1_A488_IgG2b_Antibody_1_062620](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/RMG2b-1_A488_IgG2b_Antibody_1_062620.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with CD4 (clone OKT4) (filled histogram) or purified mouse IgG2b (clone MPC-11) isotype control (open histogram) followed by anti-mouse IgG2b (clone RMG2b-1) Alexa Fluor® 488. -
C57BL/6 mouse frozen brain section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature, permeabilized with 0.5% Triton X-100 for ten minutes and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of anti-GFAP (clone 2E1.E9) purified overnight at 4°C, followed by 2.5 µg/mL of anti-mouse IgG2b (clone RMG2b-1) Alexa Fluor® 488 (green) for 1 hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with the MetaMorph® software.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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406717 | 25 µg | 83 CHF | ||||
406718 | 100 µg | 176 CHF |
The RMG2b-1 monoclonal antibody reacts with immunoglobulin G2b (IgG2b) in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. The RMG2b-1 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse Ig cocktail
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
IHC-F - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Blue Laser (488 nm)
- Product Citations
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- RRID
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AB_2876732 (BioLegend Cat. No. 406717)
AB_2876732 (BioLegend Cat. No. 406718)
Related FAQs
Other Formats
View All IgG2b Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse IgG2b | RMG2b-1 | ELISA,FC |
Biotin anti-mouse IgG2b | RMG2b-1 | FC,ELISA |
FITC anti-mouse IgG2b | RMG2b-1 | FC |
PE anti-mouse IgG2b | RMG2b-1 | FC |
Alexa Fluor® 594 anti-mouse IgG2b | RMG2b-1 | IHC-F,IHC-P |
PE/Cyanine7 anti-mouse IgG2b | RMG2b-1 | FC |
APC anti-mouse IgG2b | RMG2b-1 | FC |
Alexa Fluor® 647 anti-mouse IgG2b | RMG2b-1 | FC,IHC-F,IHC-P |
Alexa Fluor® 488 anti-mouse IgG2b | RMG2b-1 | FC,IHC-F |
TotalSeq™-A1168 anti-mouse IgG2b | RMG2b-1 | PG |
TotalSeq™-C1168 anti-mouse IgG2b | RMG2b-1 | PG |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse IgG2b
-
Biotin anti-mouse IgG2b
-
FITC anti-mouse IgG2b
-
PE anti-mouse IgG2b
Human peripheral blood lymphocytes were stained with purifie... -
Alexa Fluor® 594 anti-mouse IgG2b
C57BL/6 mouse frozen brain section was fixed with 4% parafor... IHC staining of Alexa Fluor® 594 anti-mouse IgG2b on formali... -
PE/Cyanine7 anti-mouse IgG2b
Human peripheral blood lymphocytes were stained with purifie... -
APC anti-mouse IgG2b
Human peripheral blood lymphocytes were stained with purifie... -
Alexa Fluor® 647 anti-mouse IgG2b
Human peripheral blood lymphocytes were stained with CD4 (cl... C57BL/6 mouse frozen brain section was fixed with 4% parafor... IHC staining of Alexa Fluor® 647 anti-mouse IgG2b antibody o... -
Alexa Fluor® 488 anti-mouse IgG2b
Human peripheral blood lymphocytes were stained with CD4 (cl... C57BL/6 mouse frozen brain section was fixed with 4% parafor... -
TotalSeq™-A1168 anti-mouse IgG2b
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TotalSeq™-C1168 anti-mouse IgG2b
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