- Clone
- OX-33 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Leukocyte common antigen, high molecular weight form
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![OX-33_Alx488_021308 OX-33_Alx488_021308](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/OX-33_Alx488_021308.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
LOU rat splenocytes stained with OX-33 Alexa Fluor® 488
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
202310 | 100 µg | 229 CHF |
CD45 is a protein tyrosine phosphatase with multiple isoforms differing as a result of alternative splicing of the extracellular domain and glycosylation. CD45 is expressed on all hematopoietic cells except erythrocytes and platelets. CD45RA is one of the CD45 isoforms with a molecular weight of 200-220 kD. It is expressed almost exclusively on B cells. CD45 functions in signal transduction through T and B cell antigen receptors. CD45 has been shown to interact with various proteins, including galectin-1, CD2, CD3, and CD4.
Product DetailsProduct Details
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Leukocyte common antigen purified from rat splenocytes.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1.
-
Application References
(PubMed link indicates BioLegend citation) -
- Smith T, et al. 2000. Nature Med. 6:62. (IHC)
- RRID
-
AB_572043 (BioLegend Cat. No. 202310)
Antigen Details
- Structure
- Protein tyrosine phosphatase, type I transmembrane protein, hign molecular weight isoform approximately 200-220 kD
- Distribution
-
Primarily on B lymphocytes
- Function
- B cell receptor signal transduction
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4
- Cell Type
- B cells
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Inhibitory Molecules
- Molecular Family
- CD Molecules
- Antigen References
-
1. Sunderland CA, et al. 1979. Eur. J. Immunol. 9:155.
2. Woolett GR, et al. 1985. Eur. J. Immunol. 15:168. - Gene ID
- 24699 View all products for this Gene ID
- UniProt
- View information about CD45RA on UniProt.org
Other Formats
View All CD45RA Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-rat CD45RA | OX-33 | FC,IHC-F |
Biotin anti-rat CD45RA | OX-33 | FC |
FITC anti-rat CD45RA | OX-33 | FC |
PE anti-rat CD45RA | OX-33 | FC |
Alexa Fluor® 488 anti-rat CD45RA | OX-33 | FC |
APC anti-rat CD45RA | OX-33 | FC |
PerCP/Cyanine5.5 anti-rat CD45RA | OX-33 | FC |
PE/Cyanine7 anti-rat CD45RA | OX-33 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-rat CD45RA
LOU rat splenocytes stained with purified OX-33, followed by... -
Biotin anti-rat CD45RA
LOU rat splenocytes stained with biotinylated OX-33, followe... -
FITC anti-rat CD45RA
LOU rat splenocytes stained with OX-33 FITC -
PE anti-rat CD45RA
LOU rat splenocytes stained with OX-33 PE -
Alexa Fluor® 488 anti-rat CD45RA
LOU rat splenocytes stained with OX-33 Alexa Fluor® 488 -
APC anti-rat CD45RA
LOU rat splenocytes stained with OX-33 APC -
PerCP/Cyanine5.5 anti-rat CD45RA
LOU rat splenocytes were stained with CD45RA (clone OX-33) P... -
PE/Cyanine7 anti-rat CD45RA
LOU rat splenocytes were stained with CD45RA (clone OX-33) P...
Follow Us