- Clone
- 11-26c.2a (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Immunoglobulin D
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A_11-26ca_A594_IgD_Antibody_040820 A_11-26ca_A594_IgD_Antibody_040820](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/11-26ca_A594_IgD_Antibody_040820.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS + rat/mouse/hamster serum for 30 minutes at room temperature. Then the section was stained with 5 µg/ml of Alexa Fluor® 594 anti-mouse IgD (Clone 11-26c-2a) (red) and 5 µg/ml Alexa Fluor® 647 anti-mouse CD3 (green) overnight at 4°C. The image was captured by 10X objective. -
Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen section was processed according to the Ce3DTM Tissue Clearing Kit protocol (cat. no. 427701). The section was costained with anti-mouse CD3ε Antibody (clone 500A2) Alexa Fluor® 488 at 5 µg/mL (green), anti-mouse IgD Antibody (clone 11-26c.2a) Alexa Fluor® 594 at 5 µg/mL (yellow), and anti-mouse CD172a (SIRPα) Antibody (clone P84) Alexa Fluor® 647 at 5 µg/mL (magenta) and counterstained with DAPI (blue). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
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Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
405740 | 100 µg | 323 CHF |
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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IHC-F - Quality tested
3D IHC - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.0 - 10 μg/mL is recommended. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Application Notes
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The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3, and spatial biology (IBEX)10,11.
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Application References
(PubMed link indicates BioLegend citation) -
- Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC)
- Weih D, et al. 2001. J. Immunol. 167:1909. (IHC)
- Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
- Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed
- Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
- Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
- Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed
- Park SY, et al. 2013. J. Immunol. 190:1094. PubMed
- Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed
- Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
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- RRID
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AB_2565572 (BioLegend Cat. No. 405740)
Antigen Details
- Structure
- Ig family
- Distribution
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B cells
- Function
- B cell differentiation
- Cell Type
- B cells
- Biology Area
- Immunology
- Gene ID
- 380797 View all products for this Gene ID
- UniProt
- View information about IgD on UniProt.org
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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FITC anti-mouse IgD
C57BL/6 mouse splenocytes stained with 11-26c.2a FITC -
PE anti-mouse IgD
C57BL/6 splenocytes stained with 11-26c.2a PE -
Purified anti-mouse IgD
C57BL/6 mouse splenocytes stained with purified 11-26c.2a, f... -
PerCP anti-mouse IgD
C57BL/6 mouse splenocytes were stained with CD45R/B220 APC a... -
Biotin anti-mouse IgD
C57BL/6 splenocytes were stained with CD45R/B220 APC and bio... -
Brilliant Violet 711™ anti-mouse IgD
C57BL/6 mouse splenocytes were stained with B220 APC and IgD... -
Alexa Fluor® 700 anti-mouse IgD
C57BL/6 mouse splenocytes were stained with B220 FITC and Ig... Confocal image of C57BL/6 mouse spleen sample acquired using... Confocal image of C57BL/6 mouse small intestine sample acqui... -
Alexa Fluor® 647 anti-mouse IgD
C57BL/6 splenocytes stained with 11-26c.2a Alexa Fluor® 647 Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen secti... -
PerCP/Cyanine5.5 anti-mouse IgD
C57BL/6 splenocytes stained with 11-26c.2a PerCP/Cyanine5.5 -
Pacific Blue™ anti-mouse IgD
C57BL/6 splenocytes stained with 11-26c.2a Pacific Blue&trad... C57BL/6 splenocytes stained with rat IgG2a Pacific Blue&trad... -
APC anti-mouse IgD
C57BL/6 splenocytes stained with 11-26c.2a APC -
APC/Cyanine7 anti-mouse IgD
C57BL/6 splenocytes stained with 11-26c.2a APC/Cyanine7 -
Alexa Fluor® 488 anti-mouse IgD
C57BL/6 splenocytes were stained with B220 APC and IgD (11-2... Mice were injected subcutaneously with sheep red blood cells... -
PE/Cyanine7 anti-mouse IgD
C57BL/6 splenocytes were stained with CD45R/B220 FITC and Ig... -
Brilliant Violet 650™ anti-mouse IgD
C57BL/6 splenocytes were stained with CD45R/B220 APC and IgD... -
Brilliant Violet 510™ anti-mouse IgD
C57BL/6 splenocytes were stained with CD45R/B220 APC and IgD... -
Brilliant Violet 421™ anti-mouse IgD
C57BL/6 splenocytes were stained with CD45R/B220 APC and IgD... -
Brilliant Violet 605™ anti-mouse IgD
C57BL/6 splenocytes were stained with CD45R/B220 APC and IgD... -
Purified anti-mouse IgD (Maxpar® Ready)
C57BL/6 mouse splenocytes stained with 176Yb-anti-CD45R/B220... -
Alexa Fluor® 594 anti-mouse IgD
C57BL/6 mouse frozen spleen section was fixed with 4% parafo... Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen secti... -
PE/Dazzle™ 594 anti-mouse IgD
C57BL/6 mouse splenocytes were stained with B220 APC and IgD... -
APC/Fire™ 750 anti-mouse IgD
C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC ... -
TotalSeq™-A0571 anti-mouse IgD
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TotalSeq™-C0571 anti-mouse IgD
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Spark NIR™ 685 anti-mouse IgD
C57BL/6 splenocytes were stained with anti-mouse CD45R/B220 ... -
TotalSeq™-B0571 anti-mouse IgD Antibody
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Spark Violet™ 423 anti-mouse IgD
C57BL/6 splenocytes were stained with anti-mouse/human CD45R... -
PE/Cyanine5 anti-mouse IgD
C57BL/6 mouse splenocytes were stained with anti-mouse B220 ... -
Brilliant Violet 785™ anti-mouse IgD
C57BL/6 mouse splenocytes were stained with anti-mouse B220 ... -
Spark PLUS UV™ 395 anti-mouse IgD
C57BL/6 mouse splenocytes were stained with anti-mouse CD45R...
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