- Clone
- D2-40 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Podoplanin, T1-alpha, hT1alpha-1, hT1alpha-2, PA2.26 antigen, glycoprotein 36, lung type-I cell membrane-associated glycoprotein (T1A-2)
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![D2-40_A594_Podoplanin_Antibody_030719 D2-40_A594_Podoplanin_Antibody_030719](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/D2-40_A594_Podoplanin_Antibody_030719.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human paraffin-embedded breast tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Sodium Citrate H.I.E.R. 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human podoplanin (clone D2-40) Alexa Fluor® 594 (red) at 4°C overnight. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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916607 | 25 µg | 171 CHF | ||||
916608 | 100 µg | 311 CHF |
Podoplanin is expressed by a variety of normal cells, which include breast and prostate myopepithelial cells, follicular dendritic cells, basal keratinocytes of the skin and cervix (focal), type I pneumocytes, ependymal cells, and fetal cerebral germinal matrix cells. Podoplanin is a marker of lymphatic differentiation because it is expressed by normal human lymphatic endothelium, but not by vascular endothelium. It is diagnostically a useful marker for the evaluation of a variety of neoplasms, including Kaposi sarcoma mesothelioma, testicular germ cell tumors, and cutanneous sebaceous neoplasms.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- M2A protein derived from human germ cell tumors.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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IHC-P - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Application Notes
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Additional reported applications (for the relevant formats) include: ELISA Detection5 and immunocytochemistry4.
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Application References
(PubMed link indicates BioLegend citation) -
- Hamanaka T, et al. 2011. Invest. Ophthalmol. Vis. Sci. 52:8849. (IHC-P)
- Choi WW, et al. 2005. Mod. Pathol. 18:143. (IHC-P)
- Chu AY, et al. 2005. Mod. Pathol. 18:105. (IHC-P) PubMed
- Yoon C, et al. 2008. Blood 112:1129. (ICC) PubMed
- Schacht V, et al. 2005. American Journal of Pathology 166:913. (ELISA Detection, WB) PubMed
- Marks A, et al. 1999. British Journal of Cancer 80:569.
- RRID
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AB_2801252 (BioLegend Cat. No. 916607)
AB_2801253 (BioLegend Cat. No. 916608)
Antigen Details
- Structure
- 35 kD.
- Distribution
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Membrane and plasma membrane.
- Function
- May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport and does not function as a water channel or as a regulator of aquaporin-type water channels.
- Interaction
- Cytoskeletal signaling.
- Biology Area
- Cancer Biomarkers, Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
- Gene ID
- 10630 View all products for this Gene ID
- UniProt
- View information about Podoplanin on UniProt.org
Related FAQs
Other Formats
View All Podoplanin Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
D2-40 Lymphatic Endothelial Marker Monoclonal Antibody | D2-40 | IHC |
Purified anti-Podoplanin (Lymphatic Endothelial Marker) | D2-40 | IHC-P,WB,ICFC,ICC,ELISA Capture |
Alexa Fluor® 594 anti-Podoplanin/Lymphatic Endothelial Marker | D2-40 | IHC-P |
Alexa Fluor® 647 anti-Podoplanin (Lymphatic Endothelial Marker) | D2-40 | IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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D2-40 Lymphatic Endothelial Marker Monoclonal Antibody
Immunohistochemistry showing lymphatic channels with stainin... -
Purified anti-Podoplanin (Lymphatic Endothelial Marker)
Western blot analysis of cell lysates from HeLa using Podopl... IHC staining of purified anti-Podoplanin (Lymphatic Endothel... IHC staining of purified anti-Podoplanin (Lymphatic Endothel... LN319 cells (positive control, filled histogram), or HeLa ce... HeLa cells (negative control, top panels), or LN319 cells (p... -
Alexa Fluor® 594 anti-Podoplanin/Lymphatic Endothelial Marker
Human paraffin-embedded breast tissue slices were prepared ... -
Alexa Fluor® 647 anti-Podoplanin (Lymphatic Endothelial Marker)
Human paraffin-embedded breast tissue slices were prepared w...
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