- Clone
- A18073A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Rab8, Rab8A
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A18073A_A594_RAB8_Antibody_1_120419 A18073A_A594_RAB8_Antibody_1_120419](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A18073A_A594_RAB8_Antibody_1_120419.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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ICC staining of Alexa Fluor® 594 anti-Rab8 antibody (clone A18073A) on HeLa cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 2 µg/mL of the primary antibody overnight at 4°C. Flash Phalloidin™ Green 488 (Cat. No. 424201) was used to stain the F-actin cytoskeleton. The slide was mounted with fluoromount-G with DAPI. The image was captured with a 60X objective. Scale bar: 20 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
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865405 | 25 µg | 153 CHF | ||||
865406 | 100 µg | 323 CHF |
Rab8 is a small GTP/GDP binding protein that belongs to the RAS superfamily. It is well established that Rab8 is an important participant of macroautophagy, and is essential for several apical transport pathways. Recruitment of Rab8a and TBK1 by the autophagy receptor optineurin initiates the autophagy process at specific protein aggregate site, which leads to the clearance of aggregated protein. Recent studies on C9ORF72, the gene that most commonly mutated in Amyotrophic Lateral Sclerosis and Frontotemporal Dementia (ALS-FTD), show that the C9ORF72/SMCR8/WDR41 complex acts as a GDP/GTP exchange factor for Rab8a, which facilitates the interaction between Rab8a and the autophagy receptor optineurin4.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human Rab8A
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.0 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Application Notes
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This clone cross-react with Rab8A and Rab8B.
- RRID
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AB_2820160 (BioLegend Cat. No. 865405)
AB_2820161 (BioLegend Cat. No. 865406)
Antigen Details
- Structure
- Rab8 is a 207 amino acid protein with an apparent molecular mass of ~24 kD.
- Distribution
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Tissue Distribution: ubiquitously expressed
Cellular Distribution: Plasma membrane, cytoskeleton, cytosol, Golgi Apparatus, and endosome. - Function
- Rab8 is a GTPase that plays an important role in intracellular membrane trafficking.
- Interaction
- MICALL1, MICALL2, Optineurin, MAP4K2
- Cell Type
- Astrocytes, Dopaminergic Neurons, GABAergic Neurons, Glutamatergic Neurons, Mature Neurons, Microglia, Neural Stem Cells, Neurons, Oligodendrocytes
- Biology Area
- Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation, Protein Trafficking and Clearance, Synaptic Biology
- Molecular Family
- Autophagosome Markers, Cytoskeletal Proteins, Organelle Markers, Tumor Suppressors
- Antigen References
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- Zhang X and Gao N. 2016. Small GTPases. 7:59
- Just WW and Peränen J. 2016. Biochim Biophys Acta. 1863:1006
- Ao X, et al. 2014. Cell Death Differ. 21(3):348
- Corbier C and Sellier C. 2017. Small GTPases. 8:181
- Gene ID
- 4218 View all products for this Gene ID 17274 View all products for this Gene ID 117103 View all products for this Gene ID
- UniProt
- View information about Rab8 on UniProt.org
Other Formats
View All RAB8 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Rab8 | A18073A | WB,ICC |
Biotin anti-Rab8 | A18073A | ICC |
Alexa Fluor® 594 anti-Rab8 | A18073A | ICC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Rab8
Western blot of purified anti-Rab8 antibody (clone A18073A).... ICC staining of purified anti-Rab8 antibody (clone A18073A) ... -
Biotin anti-Rab8
ICC staining of Biotin anti-Rab8 antibody (clone A18073A) on... -
Alexa Fluor® 594 anti-Rab8
ICC staining of Alexa Fluor® 594 anti-Rab8 antibody (clone A...
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