- Clone
- MRG2a-83 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Immunoglobulin G2a
- Isotype
- Mouse IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![MRG2a-83_IgG20_Antibody_1_062323 MRG2a-83_IgG20_Antibody_1_062323](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/MRG2a-83_IgG20_Antibody_1_062323.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 frozen mouse spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of purified anti-mouse CD8a (clone 53-6.7), 10 µg/mL of Alexa Fluor® 647 anti-CD20 (green) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor® 594 anti-rat IgG2a (clone MRG2a-83) (red) for two hours at room temperature. The image was captured by 10X objective. -
HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with MeOH for 10 minutes, and blocked with 5% FBS for 1 hour. Cells were then stained with 5.0 µg/mL of purified rat anti-histone H3 phosphorylated (Ser28) (Cat. No. 641002) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-rat IgG2a (clone MRG2a-83) at 2.5 µg/mL. Nuclei were counterstained with DAPI (blue, panel A) (Cat. No. 422801). The images were captured with a 40X objective. Scale bar = 50 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
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407509 | 100 µg | 235 CHF |
The MRG2a-83 monoclonal antibody reacts with rat immunoglobulin G2a (IgG2a) in all tested rat strains (Lou, Lou/Ws1/M, Lewis, Wistar, DA, Sprague-Dawley). It does not react with other isotypes. The MRG2a-83 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.
Product DetailsProduct Details
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Mixed rat Igs
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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IHC-F - Quality tested
ICC - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescence staining. For immunohistochemistry, a concentration range of 2-5 μg/mL is suggested. For immunocytochemistry, a concentration range of 2.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses -
Application References
(PubMed link indicates BioLegend citation) -
- Inoue S, et al. 2006. Cancer Research 66:7741.
- RRID
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AB_2650845 (BioLegend Cat. No. 407509)
Related FAQs
Other Formats
View All IgG2a Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-rat IgG2a | MRG2a-83 | ELISA,FC |
Biotin anti-rat IgG2a | MRG2a-83 | ELISA,FC |
FITC anti-rat IgG2a | MRG2a-83 | FC |
PE anti-rat IgG2a | MRG2a-83 | FC |
Alexa Fluor® 594 anti-rat IgG2a | MRG2a-83 | IHC-F,ICC |
Alexa Fluor® 647 anti-rat IgG2a | MRG2a-83 | FC,IHC-F |
Alexa Fluor® 488 anti-rat IgG2a | MRG2a-83 | FC,IHC-F |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-rat IgG2a
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Biotin anti-rat IgG2a
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FITC anti-rat IgG2a
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PE anti-rat IgG2a
C57BL/6 mouse splenocytes were stained with purified CD19 fo... -
Alexa Fluor® 594 anti-rat IgG2a
C57BL/6 frozen mouse spleen section was fixed with 4% parafo... HeLa cells were fixed with 4% paraformaldehyde for 10 minute... -
Alexa Fluor® 647 anti-rat IgG2a
C57BL/6 mouse splenocytes were stained with purified CD19 (c... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
Alexa Fluor® 488 anti-rat IgG2a
C57BL/6 mouse splenocytes were stained with purified anti-mo... C57BL/6 mouse frozen spleen section was fixed with 4% parafo...
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