- Clone
- 2F1-1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Actin, cytoplasmic 1
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![2F1dash1_AF647_beta_actin_Antibody_IHCP_110716 2F1dash1_AF647_beta_actin_Antibody_IHCP_110716](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/2F1dash1_AF647_beta_actin_Antibody_IHCP_110716.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human paraffin-embedded colon tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Tris-Buffered Saline 20X (1.0M, pH7.4) at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween20 twice for five minutes, permeabilized with 0.5% Triton X-100 for ten minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 5 µg/ml of anti-β-actin (clone 2F1-1) Alexa Fluor® 647 (green) and anti-mouse/human CD44 (clone IM7) Alexa Fluor® 594 (red) at 4°C overnight. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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643810 | 100 µg | 265 CHF |
β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting).
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
IHC-P - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.25 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
The binding of this antibody to its target is sensitive to salt concentration. For consistent results, please use TBS/T buffer for Western blotting that contains 0.15 M NaCl as indicated in the BioLegend recommended protocol.
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Application References
(PubMed link indicates BioLegend citation) -
- Lawson BR, et al. 2007. J. Immunol. 178:5366.
- Joyce CW, et al. 2006. J. Biol. Chem. 281:33053.
- Yanagiya T,et al.2007.Obesity.15:572.
- KishidaT,et al. 2007.J. Immunol. 179:8554.
- Ouimet M, et al. 2008. Arterisocler Thromb Vasc Biol. 28:1144.
- Toltlt LJ,et al. 2008.J. Immunol. 181:2165.
- Sawada. T,et al. 2008. J. Biol. Chem. 283:26820.
- Ikeda D, et al. 2008. Endocrinology. 149:6037.
- Ikeda Y, et al. 2010. Mol Endocrinol. 24:1338. PubMed
- Kaieda S, et al. 2010. J. Biol Chem. 285:21478. PubMed
- Cambos M, et al. 2011. J. Leukoc. Biol. 89:157. PubMed
- RRID
-
AB_2632718 (BioLegend Cat. No. 643810)
Antigen Details
- Structure
- Actin, cytoplasmic 1
- Distribution
-
Ubiquitously expressed in the cytoplasm of all eukaryotic cells
- Function
- Actins are highly conserved proteins that are involved in cell motility
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Microfilaments
- Antigen References
-
1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673.
2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133.
3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687. - Gene ID
- 11461 View all products for this Gene ID
- UniProt
- View information about beta-actin on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All β-Actin Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Alexa Fluor® 594 anti-β-actin | 2F1-1 | ICC,IHC-P |
Purified anti-β-actin | 2F1-1 | WB,ICC,IHC-P |
Brilliant Violet 421™ anti-β-actin | 2F1-1 | ICC |
Direct-Blot™ HRP anti-β-actin | 2F1-1 | WB |
Alexa Fluor® 647 anti-β-actin | 2F1-1 | IHC-P |
Alexa Fluor® 488 anti-β-actin | 2F1-1 | IHC-P |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Alexa Fluor® 594 anti-β-actin
MCF7 mammary gland cells were fixed with cold methanol for t... Human paraffin-embedded intestine was prepared with standard... -
Purified anti-β-actin
Total cell lysate from HeLa cells (lane 1, 15 µg), NIN3T3 ce... Day-three cultured postnatal C57BL/6 mouse brain cells were ... IHC staining using purified anti-β-actin antibody (clone 2F1... -
Brilliant Violet 421™ anti-β-actin
NIT/3T3 cells were fixed with 1% paraformaldehyde (PFA) for ... -
Direct-Blot™ HRP anti-β-actin
Total cell lysate from HeLa cells (lane 1, 15 µg), NIH-3T3 c... -
Alexa Fluor® 647 anti-β-actin
Human paraffin-embedded colon tissue slices were prepared wi... -
Alexa Fluor® 488 anti-β-actin
Human paraffin-embedded colon tissue slices were prepared wi...
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