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Alexa Fluor® 647 anti-GATA1 Antibody

Pricing & Availability
Clone
P84F5 (See other available formats)
Regulatory Status
RUO
Other Names
GATA Binding Protein 1, Erythroid Transcription Factor, Globin Transcription Factor 1, NFE1, XLTT
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A. P84F5_Alexa-Fluor-647_GATA1_ICFC_052924
HEL cells (positive control, filled histogram) and HeLa cells (negative control, open histogram) were fixed and permeabilized using True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) and intracellularly stained with Alexa Fluor® 647 anti-GATA1 (clone P84F5) or Alexa Fluor® 647 mouse IgG2b, κ isotype control (open histogram, dashed line) (representative histogram for both cell lines) (Cat. No. 400330).
  • A. P84F5_Alexa-Fluor-647_GATA1_ICFC_052924
    HEL cells (positive control, filled histogram) and HeLa cells (negative control, open histogram) were fixed and permeabilized using True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) and intracellularly stained with Alexa Fluor® 647 anti-GATA1 (clone P84F5) or Alexa Fluor® 647 mouse IgG2b, κ isotype control (open histogram, dashed line) (representative histogram for both cell lines) (Cat. No. 400330).
  • B. P84F5_Alexa-Fluor-647_GATA1_ICC_052924
    HEL cells were fixed with Fixation Buffer (Cat. No. 420801) and permeabilized with ice-cold methanol and then blocked with 5% FBS. Cells were then stained with Alexa Fluor® 647 mouse IgG2b, κ isotype control (Cat. No. 400330) (panel A) or Alexa Fluor® 647 anti-GATA1 (clone P84F5) (panel B) overnight. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 µm
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939105 25 µg 180 CHF
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939106 100 µg 450 CHF
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Description

The GATA family of transcription factors is comprised of six members characterized by their binding to the consensus DNA sequence (T/A)GATA(A/G). GATA1, which was the initially described member of the GATA family, is predominantly expressed in cells of the myeloid lineage such as erythrocytes, eosinophils, mast cells, and megacaryocytes. Over the course of erythroid differentiation, GATA2 activates expression of the functionally distinct GATA1; promoter-occupied GATA2 is replaced/exchanged at certain promoters with the GATA1 transcription factor, a process known as the GATA switch. Genes induced by GATA1 include HBB, HBG1/2, ALAS2 and HMBS. Haploinsufficient heterozygous GATA1 knockout mice spontaneously develop erythroblastic leukemia, and GATA1-null mice die early in development due to profound anemia.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length recombinant human GATA1 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

For immunocytochemistry (ICC) use we recommend cell fixation/permeabilization with either PFA/Triton X-100 or PFA/methanol. Cell Fixation/permeabilization with methanol alone is not suitable for this antibody.

When using this clone for ICFC, we recommend using the True-Nuclear™ Transcription Factor Buffer Set.

Antigen Details

Structure
GATA1 is a 413 amino acid protein with a predicted molecular weight of 43 kD. Additional isoforms of 335 (35 kD) and 330 (34 kD) amino acids have been described.
Distribution

Erythroid and megakaryocytic cells/Nucleus

Function
Transcription Factor/Erythroid cell development and differentiation
Cell Type
Erythrocytes, Megakaryocytes
Biology Area
Cell Biology, Stem Cells, Transcription Factors
Antigen References
  1. Shimizu R, et al. 2008. Nat Rev Cancer. 8:279-87.
  2. Bresnick EH, et al. 2010. J Biol Chem. 285:31087-93.
  3. Katsumura, et al. 2017. Blood. 129:2092-2102.
Gene ID
2623 View all products for this Gene ID
UniProt
View information about GATA1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05.29.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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