- Clone
- QA19A61 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Lewis X, 3-FAL, 3-FL, LNFP III, Lex , SSEA-1, X-hapten
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![QA19A61_AlexaFluor647_CD15_Antibody_1_050322 QA19A61_AlexaFluor647_CD15_Antibody_1_050322](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/QA19A61_AlexaFluor647_CD15_Antibody_1_050322.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood granulocytes stained with anti-human CD15 recombinant (clone QA19A61) Alexa Fluor® 647 (filled histogram) or mouse IgG1, κ Alexa Fluor® 647 isotype control (open histogram). -
Human paraffin-embedded spleen tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween-20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human CD15 recombinant (clone QA19A61) Alexa Fluor® 647 (red) and anti-human CD45RA (clone HI100) Alexa Fluor® 594 (green) at 4°C overnight. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
376303 | 25 tests | 124 CHF | ||||
376304 | 100 tests | 265 CHF |
CD15 is 3-fucosyl-N-acetyllactosamine (3-FAL), also known as Lewis X, 3-FAL, X-hapten, and SSEA-1. CD15 is expressed on granulocytes and monocytes. It has also been shown to be expressed on Langerhans cells and some malignant cells. CD15 has been implicated in adhesion, as well as chemotaxis, phagocytosis, and bactericidal activity.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
IHC-P - Verified
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- RRID
-
AB_2922598 (BioLegend Cat. No. 376303)
AB_2922598 (BioLegend Cat. No. 376304)
Antigen Details
- Structure
- Poly-N-acetyllactosamine
- Distribution
-
Neutrophils, eosinophils, monocytes
- Function
- Adhesion
- Cell Type
- Embryonic Stem Cells, Eosinophils, Monocytes, Neural Stem Cells, Neutrophils
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
- Stocks SC, et al. 1990. Biochem J. 268:275-80.
- Gene ID
- 2526 View all products for this Gene ID
- UniProt
- View information about CD15 on UniProt.org
Other Formats
View All CD15 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD15 Recombinant Antibody | QA19A61 | FC,IHC-P |
Alexa Fluor® 647 anti-human CD15 Recombinant Antibody | QA19A61 | FC,IHC-P |
Alexa Fluor® 594 anti-human CD15 Recombinant Antibody | QA19A61 | IHC-P |
PE anti-human CD15 Recombinant Antibody | QA19A61 | FC |
Spark Violet™ 423 anti-human CD15 Recombinant Antibody | QA19A61 | FC,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD15 Recombinant Antibody
Human paraffin-embedded spleen tissue slices were prepared w... Human lysed washed blood stained with purified anti-human CD... -
Alexa Fluor® 647 anti-human CD15 Recombinant Antibody
Human peripheral blood granulocytes stained with anti-human ... Human paraffin-embedded spleen tissue slices were prepared w... -
Alexa Fluor® 594 anti-human CD15 Recombinant Antibody
IHC staining of anti-human CD15 recombinant (clone QA19A61) ... -
PE anti-human CD15 Recombinant Antibody
Human peripheral blood was stained with anti-human CD11b (cl... -
Spark Violet™ 423 anti-human CD15 Recombinant Antibody
Human peripheral blood was stained with anti-human CD11b (cl... IHC staining of anti-human CD15 recombinant (clone QA19A61) ...
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