- Clone
- P44-8 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- NKp44, NCR2, Activating NK receptor NKp44, natural cytotoxicity triggering receptor 2. lymphocyte antigen 95 homolog
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Human PBMCs were stimulated with rhIL-2 for 9 days, then stained with CD56 PE and P44-8 Alexa Fluor® 647
Cat # | Size | Price | Quantity Check Availability | Save | ||
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325112 | 100 tests | 297 CHF |
CD336 is also known as activating NK receptor NKp44 (NKp44), natural cytotoxicity triggering receptor 2, and lymphocyte antigen 95 homolog. It is a type I transmembrane protein, member of the natural cytotoxicity receptor family that contains one immunoglobulin-like domain. NKp44 has an apparent molecular weight of 44 kD and three isoforms are produced by alternative splicing. NKp44 is expressed on IL-2 activated NK cells and a subset of γ/δ T cells. NKp44 enhances NK cell mediated cytolysis of virus infected cells and tumor cells. NKp44 has been shown to associate with the intracellular adaptor DAP12.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- recombinant human NKp44
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
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The p44-8 antibody against human NKp44 has been shown to be useful for flow cytometry, stimulation of human NK cells via NKp44 in a redirected lysis assay, and blocking of NKp44 function in solution. Additional reported applications (for the relevant formats) include: stimulation of human NK cells via NKp44 in a redirected lysis assay, and blocking of NKp44 function in solution1,2. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 325104).
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Application References
(PubMed link indicates BioLegend citation) - Product Citations
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- RRID
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AB_2149431 (BioLegend Cat. No. 325112)
Antigen Details
- Structure
- Type I transmembrane protein, member of the natural cytotoxicity receptor family, contains one immunoglobulin-like domain. Approximately 44 kD. Three isoforms produced by alternative splicing.
- Distribution
-
IL-2 activated NK cells and cell lines, some γ/δ T cells activated in vitro
- Function
- NK cell triggering of cytolysis toward tumor targets and other target cells deficient in MHC class I molecules
- Interaction
- DAP12
- Cell Type
- NK cells, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
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1. Cantoni C, et al. 1999. J. Exp. Med. 189:787.
2. Allcock RJN, et al. 2003. Eur. J. Immunol. 33:567.
3. Cantoni C, et al. 2003. Structure. 11:725. - Gene ID
- 9436 View all products for this Gene ID
- UniProt
- View information about CD336 on UniProt.org
Related FAQs
Other Formats
View All CD336 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD336 (NKp44) | P44-8 | FC,Block |
Biotin anti-human CD336 (NKp44) | P44-8 | FC |
PE anti-human CD336 (NKp44) | P44-8 | FC |
APC anti-human CD336 (NKp44) | P44-8 | FC |
Alexa Fluor® 647 anti-human CD336 (NKp44) | P44-8 | FC |
PerCP/Cyanine5.5 anti-human CD336 (NKp44) | P44-8 | FC |
PE/Cyanine7 anti-human CD336 (NKp44) | P44-8 | FC |
TotalSeq™-C0802 anti-human CD336 (NKp44) | P44-8 | PG |
Ultra-LEAF™ Purified anti-human CD336 (NKp44) | P44-8 | FC,Block |
TotalSeq™-A0802 anti-human CD336 (NKp44) | P44-8 | PG |
APC/Cyanine7 anti-human CD336 (NKp44) Antibody | P44-8 | FC |
KIRAVIA Blue 520™ anti-human CD336 (NKp44) | P44-8 | FC |
PE/Fire™ 810 anti-human CD336 (NKp44) | P44-8 | FC |
TotalSeq™-D0802 anti-human CD336 (NKp44) | P44-8 | PG |
Spark Red™ 718 anti-human CD336 (NKp44) (Flexi-Fluor™) | P44-8 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD336 (NKp44)
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Biotin anti-human CD336 (NKp44)
Human PBMCs were stimulated with rhIL-2 for 9 days, then sta... -
PE anti-human CD336 (NKp44)
Human PBMCs were stimulated with rhIL-2 for 7 days, then sta... -
APC anti-human CD336 (NKp44)
Human PBMCs were stimulated with rhIL-2 for 7 days, then sta... -
Alexa Fluor® 647 anti-human CD336 (NKp44)
Human PBMCs were stimulated with rhIL-2 for 9 days, then sta... -
PerCP/Cyanine5.5 anti-human CD336 (NKp44)
Human peripheral blood lymphocytes were stimulated with reco... -
PE/Cyanine7 anti-human CD336 (NKp44)
Human peripheral blood lymphocytes were stimulated with reco... -
TotalSeq™-C0802 anti-human CD336 (NKp44)
-
Ultra-LEAF™ Purified anti-human CD336 (NKp44)
-
TotalSeq™-A0802 anti-human CD336 (NKp44)
-
APC/Cyanine7 anti-human CD336 (NKp44) Antibody
Human peripheral blood lymphocytes were stimulated with reco... -
KIRAVIA Blue 520™ anti-human CD336 (NKp44)
Human PBMCs were stimulated with IL2 for 7 days. Cells were ... -
PE/Fire™ 810 anti-human CD336 (NKp44)
Human peripheral blood lymphocytes were stimulated with reco... -
TotalSeq™-D0802 anti-human CD336 (NKp44)
-
Spark Red™ 718 anti-human CD336 (NKp44) (Flexi-Fluor™)
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