- Clone
- 16B5C71 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IK1, IKZF1
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![16B5C71_AF647_Ikaros_Antibody_1_ICFC_091715 16B5C71_AF647_Ikaros_Antibody_1_ICFC_091715](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/16B5C71_AF647_Ikaros_Antibody_1_ICFC_091715.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood mononuclear cells were stained with CD19 PE, treated with the True-Nuclear™ Transcription Factor Buffer Set, and then stained with Ikaros (clone 16B5C71) Alexa Fluor® 647 (top) or mouse IgG2a, κ Alexa Fluor® 647 isotype control (bottom). -
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
368403 | 25 tests | 159 CHF | ||||
368404 | 100 tests | 358 CHF |
Ikaros, also known as IKZF1, belongs to a family of zinc-finger DNA-binding proteins that regulate lymphocyte differentiation. It has the ability to form homo- and heterodimers with other members of the Ikaros family, which include Aiolos and Helios. Ikaros can be found on a majority of fetal and adult hematopoietic cell types such as T- and B-cells, natural killer cells (NKs), monocytes/macrophages, and dendritic cells. Ikaros is a tumor suppressor and its absence or reduced expression has been reported in several leukemias.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Ikaros peptide (431-477 aa) conjugated to KLH Freund's adjuvant.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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ICFC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Red Laser (633 nm)
- Application Notes
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NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
- Product Citations
-
- RRID
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AB_2566182 (BioLegend Cat. No. 368403)
AB_2566183 (BioLegend Cat. No. 368404)
Antigen Details
- Structure
- Zinc-finger DNA binding protein, homo/heterodimer, 57.5 kD
- Distribution
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Fetal and adult hematopoietic cells.
- Function
- Regulates lymphocyte differentiation.
- Interaction
- DNA.
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Signal Transduction, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
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1. Wargnier A, et al. 1995. Proc. Natl. Acad. Sci. USA 92:6930.
2. Tonnelle C, et al. 2009. Mol. Immunol. 46:1736.
3. Wang H, et al. 2014. Pediatr. Blood Cancer 61:2230. - Gene ID
- 10320 View all products for this Gene ID
- UniProt
- View information about Ikaros on UniProt.org
Related FAQs
Other Formats
View All Ikaros Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human Ikaros | 16B5C71 | WB,ChIP |
Alexa Fluor® 647 anti-human Ikaros | 16B5C71 | ICFC |
Brilliant Violet 421™ anti-human Ikaros | 16B5C71 | ICFC |
Alexa Fluor® 488 anti-human Ikaros | 16B5C71 | ICFC |
PE anti-human Ikaros | 16B5C71 | ICFC |
APC anti-human Ikaros | 16B5C71 | ICFC |
PE/Cyanine7 anti-human Ikaros | 16B5C71 | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human Ikaros
Total lysates (15 µg protein) from Jurkat (lane 1) and HeLa ... Chromatin Immunoprecipitations (ChIP) were done with fixed a... Chromatin Immunoprecipitations (ChIP) were done with fixed a... -
Alexa Fluor® 647 anti-human Ikaros
Human peripheral blood mononuclear cells were stained with C... -
Brilliant Violet 421™ anti-human Ikaros
Human peripheral blood mononuclear cells were stained with C... -
Alexa Fluor® 488 anti-human Ikaros
Human peripheral blood mononuclear cells were stained with C... -
PE anti-human Ikaros
Human peripheral blood mononuclear cells were stained with C... -
APC anti-human Ikaros
Human peripheral blood mononuclear cells were stained with C... -
PE/Cyanine7 anti-human Ikaros
Human peripheral blood mononuclear cells were stained with C...
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