- Clone
- A15158B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Signal transducer and activator of transcription 1 (STAT1), Transcription factor ISGF-3 components p91/p84
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A15158B_A647_PhosphoSer727_Antibody_1_032017 A15158B_A647_PhosphoSer727_Antibody_1_032017](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A15158B_A647_PhosphoSer727_Antibody_1_032017.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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HeLa cells were stimulated with (filled histogram) or without (open histogram) nocodozole for 24 hours, fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, then intracellularly stained with anti-STAT1 Phospho (Ser727) antibody (clone A15158B) Alexa Fluor™ 647. -
Human peripheral blood lymphocytes were stimulated with (filled histogram) or without (open histogram) Cell Activation Cocktail (without Brefeldin A) for 15 minutes, fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, then intracellularly stained with anti-STAT1 Phospho (Ser727) antibody (clone A15158B) Alexa Fluor™ 647.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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686411 | 25 tests | 141 CHF | ||||
686412 | 100 tests | 335 CHF |
STAT1, also known as signal transduction and activator of transcription 1, is a ubiquitously expressed cytoplasmic protein and is activated in response to cytokine signaling, including IFN-α, IFN-γ, EGF, PDGF, and IL-6. Upon activation, STAT1 is phosphorylated by receptor-associated kinases, translocates to the nucleus, and functions as a transcription factor. Two isoforms of STAT1, with apparent molecular weights of 88 and 91 kD, exist as a result of alternative RNA processing. STAT1 is involved in IFN-mediated immune responses, and STAT1-deficient mice are highly sensitive to bacterial and viral infections.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human STAT1 peptide phosphorylated at Ser 727.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Product Citations
-
- RRID
-
AB_2650785 (BioLegend Cat. No. 686411)
AB_2650786 (BioLegend Cat. No. 686412)
Antigen Details
- Structure
- 750 amino acids, predicted molecular weight of 87 kD; contains a SH2 domain responsible for homodimerization or heterodimerization.
- Distribution
-
Translocates to the nucleus when phosphorylated.
- Function
- Phosphorylated in response to cytokine signaling by receptor-associated kinases; translocates to the nucleus to act as a transcription factor. Mediates responses to type I (IFN-α/β) and II interferon (IFN-γ), EGF, PDGF, and IL-6.
- Interaction
- Forms a homodimer or heterodimers with other family members. Interacts with FAK, MCM3, MCM5, TRADD, BRCA1, KIT, IL-27R, IL-2Rβ, IL-2Rγ, IFNαβR, and c-Src.
- Biology Area
- Cell Biology, Signal Transduction
- Molecular Family
- Nuclear Markers, Phospho-Proteins
- Antigen References
-
1. Durbin JE, et al. 1996. Cell. 84:443.
2. Darnell JE Jr, et al. 1994. Science 264:1415.
3. Chen X, et al. 1998. Cell. 93:827.
4. Ramana CV, et al. 2000. Oncogene. 19:2619. - Gene ID
- 6772 View all products for this Gene ID
- UniProt
- View information about STAT1 Phospho Ser727 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All STAT1 Phospho (Ser727) Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-STAT1 Phospho (Ser727) | A15158B | WB,ICFC,ICC,ChIP |
PE anti-STAT1 Phospho (Ser727) | A15158B | ICFC |
Alexa Fluor® 594 anti-STAT1 Phospho (Ser727) | A15158B | ICC |
Alexa Fluor® 647 anti-STAT1 Phospho (Ser727) | A15158B | ICFC |
Alexa Fluor® 488 anti-STAT1 Phospho (Ser727) | A15158B | ICFC |
PE/Cyanine7 anti-STAT1 Phospho (Ser727) | A15158B | ICFC |
PerCP/Cyanine5.5 anti-STAT1 Phospho (Ser727) | A15158B | ICFC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-STAT1 Phospho (Ser727)
Total lysates (15 µg protein) from untreated HeLa cells (lan... HeLa cells were stimulated with (filled histogram) or withou... Human peripheral blood lymphocytes were stimulated with (fil... Two aliquots of HeLa cells in suspension, treated with (top)... Total cell lysates (15 µg total protein) from NIH/3T3 cells ... Chromatin Immunoprecipitations (ChIP) were performed with cr... -
PE anti-STAT1 Phospho (Ser727)
HeLa cells were stimulated with (filled histogram) or withou... Human peripheral blood lymphocytes were stimulated with (fil... NIH/3T3 cells were treated with (filled histogram) or withou... -
Alexa Fluor® 594 anti-STAT1 Phospho (Ser727)
Two aliquots of HeLa cells in suspension, treated with 200 n... -
Alexa Fluor® 647 anti-STAT1 Phospho (Ser727)
HeLa cells were stimulated with (filled histogram) or withou... Human peripheral blood lymphocytes were stimulated with (fil... -
Alexa Fluor® 488 anti-STAT1 Phospho (Ser727)
HeLa cells were stimulated with (filled histogram) or withou... Human peripheral blood lymphocytes were stimulated with (fil... -
PE/Cyanine7 anti-STAT1 Phospho (Ser727)
HeLa cells were stimulated with (filled histogram) or withou... Human peripheral blood lymphocytes were stimulated with (fil... -
PerCP/Cyanine5.5 anti-STAT1 Phospho (Ser727)
HeLa cells were treated with (filled histogram) or without (... Human peripheral blood lymphocytes were treated with (filled...
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