- Clone
- m24 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Integrin β2 subunit, LFA -1 β subunit, β2 integrin, b2 subunit, LFA-1b, LFA-1 beta, LFA1b, LFA1 beta, beta 2 integrin subunit LFA
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![M24_APCFire750_CD11a_Antibody_110317 M24_APCFire750_CD11a_Antibody_110317](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/M24_APCFire750_CD11a_Antibody_110317.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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PMA-stimulated human peripheral blood granulocytes were stained with CD11a/CD18 (clone m24) APC/Fire™ 750 (filled histogram) or mouse IgG1, κ APC/Fire™ 750 isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
363419 | 25 tests | 171 CHF | ||||
363420 | 100 tests | 375 CHF |
CD11/CD18 belongs to the integrin family of proteins. It is heterodimeric cell surface receptor expressed on all leukocytes. CD18, in association with integrin α chain CD11a, CD11b, and CD11c forms LFA-1, Mac-1, and αXβ2, respectively, and plays an important role in leukocyte adhesion. CD11/CD18 complexes bind ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, and fibrinogen. Clone m24 binds the extended/open high affinity conformation of CD11a/CD18. The antibody can be used as a reporter of the activation state of the integrin receptor in response to exposure to Mg2+ or Mn2+.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Fibronectin-purified human monocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm. - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Clone m24 can be used as a reporter of the activation state of integrin receptor in response to exposure with Mg2+ or Mn2+.
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Application References
(PubMed link indicates BioLegend citation) -
- Hogg N. et al. 1985. Cell Immunol. 92:247.
- McDowall A. et al. 2003. J. Clin. Invest. 111:51.
- RRID
-
AB_2721472 (BioLegend Cat. No. 363419)
AB_2721473 (BioLegend Cat. No. 363420)
Antigen Details
- Structure
- Integrin family, 95KD, Ig superfamily
- Distribution
-
All leukocytes
- Function
- Adhesion
- Ligand/Receptor
- ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, fibrinogen
- Cell Type
- Dendritic cells, Leukocytes, Neutrophils
- Biology Area
- Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Anderson D, et al. 1987. Annu. Rev. Med. 38:175.
2. Springer T. 1994. Cell 76:301. - Gene ID
- 16408 View all products for this Gene ID 16414 View all products for this Gene ID
- UniProt
- View information about CD18 on UniProt.org
Other Formats
View All CD18 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD11a/CD18 (LFA-1)
Human peripheral blood neutrophils were stimulated with PMA ... -
Alexa Fluor® 488 anti-human CD11a/CD18 (LFA-1)
PMA-stimulated human peripheral blood granulocytes were stai... -
Brilliant Violet 421™ anti-human CD11a/CD18 (LFA-1)
PMA-stimulated human peripheral blood granulocytes were stai... -
APC anti-human CD11a/CD18 (LFA-1)
PMA-stimulated human peripheral blood granulocytes were sta... -
Alexa Fluor® 647 anti-human CD11a/CD18 (LFA-1)
PMA-stimulated human peripheral blood granulocytes were stai... -
PE anti-human CD11a/CD18 (LFA-1)
PMA-stimulated human peripheral blood granulocytes were sta... -
APC/Fire™ 750 anti-human CD11a/CD18 (LFA-1)
PMA-stimulated human peripheral blood granulocytes were sta... -
PerCP/Cyanine5.5 anti-human CD11a/CD18 LFA-1
PMA-stimulated human peripheral blood granulocytes were sta... -
PE/Cyanine7 anti-human CD11a/CD18 (LFA-1)
PMA-stimulated human peripheral blood granulocytes were sta... -
Biotin anti-human CD11a/CD18 (LFA-1)
PMA-stimulated human peripheral blood granulocytes were sta... -
FITC anti-human CD11a/CD18 (LFA-1)
PMA-stimulated human peripheral blood granulocytes were stai... -
Alexa Fluor® 700 anti-human CD11a/CD18 (LFA-1)
PMA-stimulated human peripheral blood granulocytes were stai... -
TotalSeq™-B0818 anti-human CD11a/CD18 (LFA-1)
-
TotalSeq™-C0818 anti-human CD11a/CD18 (LFA-1)
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