- Clone
- 53-5.8 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Lyt-3.2, Ly-3.2
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![53-5-8_APCFire750_CD8b-2_Antibody_122319_updated.png 53-5-8_APCFire750_CD8b-2_Antibody_122319_updated.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/53-5-8_APCFire750_CD8b-2_Antibody_122319_updated.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 splenocytes were stained with CD3 FITC and CD8b.2 (clone 53-5.8) APC/Fire™ 750 (left) or rat IgG1 APC/Fire™ 750 isotype control (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
140419 | 25 µg | 136 CHF | ||||
140420 | 100 µg | 347 CHF |
CD8b is the 32 kD β chain of CD8, also known as Lyt-3.2 or Ly-3.2. It is a member of the Ig superfamily expressed as a heterodimer with the CD8α chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in T cell activation. The antibody 53-5.8 is specific for Ly-3.2 and has low reactivity with Ly-3.1.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse thymus or spleen
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm. - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunofluorescence3, immunohistochemical staining4 of frozen tissue section using dry ice-isopentane method and immunoprecipitation5.
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Application References
(PubMed link indicates BioLegend citation) -
- Ledbetter J, et al. 1979. Immunol. Rev. 47:63.
- Ledbetter J, et al. 1980. J. Exp. Med. 152:280.
- Vremec D, et al. 2000. J. Immunol. 164:2978. (IF)
- Lawrence D, et al. 1999. J. Virol. 73:1795. (IHC)
- Bosselut R, et al. 1999. J. Exp. Med. 190:1517. (IP)
- RRID
-
AB_2819884 (BioLegend Cat. No. 140419)
AB_2819885 (BioLegend Cat. No. 140420)
Antigen Details
- Structure
- Ig superfamily, CD8β chain, 32 kD
- Distribution
-
Majority of thymocytes, cytotoxic T cell subsets
- Function
- Co-receptor of TCR
- Ligand/Receptor
- MHC class I
- Cell Type
- T cells, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, TCRs
- Antigen References
-
1. Ledbetter J, et al. 1981. J. Exp. Med. 153:1503.
2. Renard V, et al. 1996. J. Exp. Med. 184:2439. - Gene ID
- 12526 View all products for this Gene ID
- UniProt
- View information about CD8beta.2 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All CD8b.2 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD8b.2 | 53-5.8 | FC,ICC,IHC-F,IP |
FITC anti-mouse CD8b.2 | 53-5.8 | FC |
Biotin anti-mouse CD8b.2 | 53-5.8 | FC |
PE anti-mouse CD8b.2 | 53-5.8 | FC |
APC anti-mouse CD8b.2 | 53-5.8 | FC |
Pacific Blue™ anti-mouse CD8b.2 | 53-5.8 | FC |
PE/Cyanine7 anti-mouse CD8b.2 | 53-5.8 | FC |
PerCP/Cyanine5.5 anti-mouse CD8b.2 | 53-5.8 | FC |
APC/Fire™ 750 anti-mouse CD8b.2 | 53-5.8 | FC |
APC/Cyanine7 anti-mouse CD8b.2 | 53-5.8 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD8b.2
C57BL/6 mouse splenocytes stained with purified CD8b.2 (53-5... -
FITC anti-mouse CD8b.2
C57BL/6 mouse splenocytes stained with CD8b.2 (53-5.8) FITC ... -
Biotin anti-mouse CD8b.2
C57BL/6 mouse splenocytes stained with CD8a (53-6.7) APC and... -
PE anti-mouse CD8b.2
C57BL/6 mouse splenocytes stained with CD8b.2 (53-5.8) PE an... -
APC anti-mouse CD8b.2
C57BL/6 mouse splenocytes stained with CD8b.2 (53-5.8) APC a... -
Pacific Blue™ anti-mouse CD8b.2
C57BL/6 mouse splenocytes stained with 53-5.8 Pacific Blue&t... -
PE/Cyanine7 anti-mouse CD8b.2
C57BL/6 mouse splenocytes were stained with CD8a Alexa Fluor... -
PerCP/Cyanine5.5 anti-mouse CD8b.2
C57BL/6 mouse splenocytes stained with CD3e FITC and CD8b.2 ... -
APC/Fire™ 750 anti-mouse CD8b.2
C57BL/6 splenocytes were stained with CD3 FITC and CD8b.2 (c... -
APC/Cyanine7 anti-mouse CD8b.2
C57BL/6 mouse splenocytes were stained with CD3 Alexa Fluor®...
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